Results 81 to 90 of about 753,839 (313)
Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins [PDF]
, 2011 Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteinsBoth of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein ...
Gai, Qingqing +5 more
core +1 more source
Cell Reports: MethodsSummary: The heterochromatin protein HP1α (heterochromatin protein 1 alpha) phase separates in vitro and displays properties compatible with phase separation in cells.
Ziling (Kate) Zhou +3 more
doaj +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source
Does Liquid-Liquid Phase Separation Drive Peptide Folding? [PDF]
, 2020 Proline-arginine (PR) dipeptide repeats have been shown to undergo liquid-liquid phase separation and are an example of a growing number of intrinsically disordered proteins that can assemble into membraneless organelles.
Meredith R., Flanagan +2 more
core +1 more source
Amorphous-Amorphous Phase Separation in API/Polymer Formulations
Molecules, 2017 The long-term stability of pharmaceutical formulations of poorly-soluble drugs in polymers determines their bioavailability and therapeutic applicability.
Christian Luebbert +2 more
doaj +1 more source
Nanoscale Effects on Phase Separation
Nano Letters, 2017 Classical nucleation theory predicts that a binary system which is immiscible in the bulk should become miscible at the nanoscale when lowering its size below a critical size. Here we tackle the problem of miscibility in nanoalloys with a combination of ab initio and atomistic calculations, developing a statistical-mechanics approach for the free ...
PALOMARES BAEZ, JUAN PEDRO +2 more
openaire +4 more sources
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Variational models for phase separation
Interfaces and Free Boundaries, Mathematical Analysis, Computation and Applications, 2003 The paper is concerned with the asymptotic behaviour of a family of integral functionals connected with classical models for phase separation. Let \(W\) be a nonnegative, continuous, double well type energy density with minima located at \(u=0\) and \(u=1\), and with linear growth at infinity.
VITALI, ENRICO, SOLCI, MARGHERITA
openaire +4 more sources
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source