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Genetically Encoded Probes for Phosphatidic Acid

2012
In addition to forming bilayers to separate cellular compartments, lipids participate in vesicular trafficking and signal transduction. Among others, phosphatidic acid (PA) is emerging as an important signaling molecule. The spatiotemporal distribution of cellular PA appears to be tightly regulated by localized synthesis and a rapid metabolism ...
Kassas, Nawal   +6 more
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Metabolism of Phosphatidic Acid

1983
In the first edition of the Handbook of Neurochemistry, Rossiter and Strickland1 reviewed the metabolism of phosphatidic acid in relation to the overall metabolism of phosphoglycerides, and Hawthorne and Kai2 described the metabolic relationship between phosphatidic acid and phosphoinositides.
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A procedure for the preparation of [32P]phosphatidic acid

Analytical Biochemistry, 1986
The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in
R K Keller, Nancy Cafmeyer, W L Adair
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Thyroid al Synthesis of Phosphatidic Acid

Endocrinology, 1968
The enzymatic formation of phosphatidic acid from l-α-glycerophosphate and fatty acid was studied in cell free systems of calf and guinea pig thyroid. Phosphatidic acid formation was assayed by measuring the conversion of water-soluble l-α-glycerophosphate to the lipid which is extractable by organic solvents.
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Antibodies to liposomal phosphatidylserine and phosphatidic acid

Biochemistry and Cell Biology, 1990
Polyclonal antisera to phosphatidylserine or phosphatidic acid were induced in rabbits by injecting liposomes containing phosphatidylserine or phosphatidic acid and lipid A. Adsorption of antisera with liposomes containing different phospholipids revealed that some degree of reactivity with one or more phospholipids other than the immunizing ...
Carl R. Alving, Benoy Banerji
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Phosphatidic acid- and phosphatidylserine-binding proteins

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2006
Phosphatidic acid and phosphatidylserine are negatively charged abundant phospholipids with well-recognized structural roles in cellular membranes. They are also signaling lipids since their regulated formation (or appearance) can constitute an important signal for downstream responses.
Nicholas T. Ktistakis, Catherine Stace
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Transport of phosphatidic acid within the mitochondrion

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
Transfer of phosphatidic acid from the outer to the inner membrane within intact rat liver mitochondria was assessed by measuring the ratio of lipid 32P to the marker enzyme of the outer membrane, rotenone-insensitive NADH-cytochrome c reductase, in the outer and inner membrane fractions obtained after incubation of mitochondria under conditions for ...
Józef Zborowski   +2 more
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Phosphatidic Acid Phosphatases in Seed Plants [PDF]

open access: possible, 2009
Phosphatidic acid phosphatase (PAP) dephosphorylates phosphatidic acid (PA) to yield diacylglycerol (DAG) and inorganic phosphate (Pi). In seed plants, PAP functions both as a crucial enzyme for membrane lipid biosynthesis and an attenuator for PA signal.
Nakamura, Y., Ohta, H.
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Phosphatidic Acid as a Precursor of Phosphoinositide in the Thyroid

Endocrinology, 1972
Previous reports on the incorporation of 32P into thyroid slice phosphatidic acid (PA) and phosphoinositide (PI) have claimed that the specific activity (SA) of the PI is greater than that of the PA. This would be inconsistent with the generally accepted biosynthetic sequence in which PA is a precursor of PI.
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Pulmonary phosphatidic acid phosphatase. A comparative study of the aqueously dispersed phosphatidate-dependent and membrane-bound phosphatidate-dependent phosphatidic acid phosphatase activities of rat lung

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
1. The properties of the aqueously dispersed phosphatidate-dependent phosphatidic acid phosphatase (EC 3.1.3.4) activities of rat lung have been studied in microsomal and cytosol preparations and compared with the properties of the membrane-bound phosphatidate-dependent activities. 2.
Paul G. Casola   +4 more
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