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Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities
2008Phosphoinositides are a family of lipid second messengers interlinked by an extensive and highly regulated network of kinases and phosphatases. The modulation of phosphoinositide profiles can regulate numerous cancer-related pathways, including cell survival, cell proliferation, migration, integrin activation, and transcription. PtdIns(4,5)P2 is at the
Halstead, J. R. +4 more
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Archives Internationales de Physiologie, de Biochimie et de Biophysique, 1993
Phosphatidylinositol kinase (PI kinase) and phosphatidylinositol 4-phosphate kinase (PIP kinase) activities in an insect cell line (Md 66) were studied by measuring the incorporation of 32P from [gamma-32P] ATP into phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) respectively.
V B, Yadwad, R G, Downer
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Phosphatidylinositol kinase (PI kinase) and phosphatidylinositol 4-phosphate kinase (PIP kinase) activities in an insect cell line (Md 66) were studied by measuring the incorporation of 32P from [gamma-32P] ATP into phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) respectively.
V B, Yadwad, R G, Downer
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Phosphatidic acid regulation of phosphatidylinositol 4-phosphate 5-kinases
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2009Phosphatidic acid (PA) production by receptor-stimulated phospholipase D is believed to play an important role in the regulation of cell function. The second messenger function of PA remains to be elucidated. PA can bind and affect the activities of different enzymes and here we summarise the current status of activation of Type I phosphatidylinositol ...
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Formation of phosphatidylinositol‐4‐phosphate in human peripheral blood eosinophils
Clinical & Experimental Allergy, 1993SummaryTo evaluate the presence of phosphatidylinositol‐4‐phosphate in peripheral blood eosinophils, venous blood was drawn from normal healthy volunteers. The eosinophils were isolated on a Percoll gradient and were incubated with [γ3P] ATP in the presence of Mg2+.
M, Kurosawa, T, Ishizuka, Y, Shimizu
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Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1988
Subcellular fractions isolated and purified from rat brain cerebral cortices were assayed for phosphatidylinositol (PI-), phosphatidylinositol-4-phosphate (PIP-), and diacylglycerol (DG-) kinase activities in the presence of endogenous or exogenously added lipid substrates and [gamma-32P]ATP.
E B, Stubbs, J A, Kelleher, G Y, Sun
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Subcellular fractions isolated and purified from rat brain cerebral cortices were assayed for phosphatidylinositol (PI-), phosphatidylinositol-4-phosphate (PIP-), and diacylglycerol (DG-) kinase activities in the presence of endogenous or exogenously added lipid substrates and [gamma-32P]ATP.
E B, Stubbs, J A, Kelleher, G Y, Sun
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Phosphatidylinositol 4-phosphate; A minor lipid with multiple personalities
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsPhosphorylated products of phosphatidylinositol (PI), named Diphosphoinositide (DPI) and triphosphoinositide (TPI) were identified long time ago and found to exhibit high turnover rates based on their rapid 32P-phosphate labeling. The PI kinase activities that were responsible for their production were subsequently identified and found to be associated
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PA binding of phosphatidylinositol 4-phosphate 5-kinase
Advances in Enzyme Regulation, 2008Catherine, Stace +5 more
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Activation of Phosphatidylinositol 4-Phosphate 5-Kinases by Phosphatidylinositol Transfer Proteins
Biochemical Society Transactions, 2000O. Sadek +3 more
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