PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism
Cell Reports, 2019 Summary: Insulin stimulates the conversion of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) to phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), which mediates downstream cellular responses.
Diana G. Wang +14 more
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A conserved function in phosphatidylinositol metabolism for mammalian Vps13 family proteins. [PDF]
PLoS ONE, 2015 The Vps13 protein family is highly conserved in eukaryotic cells. In humans, mutations in the gene encoding the family member VPS13A lead to the neurodegenerative disorder chorea-acanthocytosis.
Jae-Sook Park +3 more
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Osh6 Revisited: Control of PS Transport by the Concerted Actions of PI4P and Sac1 Phosphatase
Frontiers in Molecular Biosciences, 2021 Osh6, a member of the oxysterol-binding protein–related protein (ORP) family, is a lipid transport protein that is involved in the transport of phosphatidylserine (PS) between the endoplasmic reticulum (ER) and the plasma membrane (PM).
Andrea Eisenreichova +3 more
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Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases [PDF]
Biochemical Journal, 1992 We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa.
Divecha, N. +2 more
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Membrane-mediated dimerization potentiates PIP5K lipid kinase activity
eLife, 2022 The phosphatidylinositol 4-phosphate 5-kinase (PIP5K) family of lipid-modifying enzymes generate the majority of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] lipids found at the plasma membrane in eukaryotic cells.
Scott D Hansen +3 more
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Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase [PDF]
, 2014 The Vps34 (vacuolar protein sorting 34) class III PI3K (phosphoinositide 3-kinase) phosphorylates PtdIns (phosphatidylinositol) at endosomal membranes to generate PtdIns(3)P that regulates membrane trafficking processes via its ability to recruit a ...
Alessi, Dario R. +10 more
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Mechanisms of Cellular Membrane Reorganization to Support Hepatitis C Virus Replication
Viruses, 2016 Like all positive-sense RNA viruses, hepatitis C virus (HCV) induces host membrane alterations for its replication termed the membranous web (MW). Assembling replication factors at a membranous structure might facilitate the processes necessary for ...
Hongliang Wang, Andrew W. Tai
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Phosphatidylinositol 4‐phosphate dynamics during phagocytosis
The FASEB Journal, 2015 Phosphoinositide levels undergo acute changes during the course of phagocytosis; phosphatidylinositol 4,5‐ bis phosphate [PtdIns(4,5)P 2 ], phosphatidylinositol 3,4,5‐ tris phosphate and phosphatidylinositol 3 ...
Roni Levin +4 more
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Editorial: Membrane lipids in T cell functions [PDF]
, 2018 Plasma membrane lipids play essential roles in regulating T cell signaling, differentiation, and effector functions. The major lipid species in the plasma membrane are glycerophospholipids, sphingolipids, and sterol lipids.
Tuosto, Loretta, Xu, Chenqi
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An HPLC procedure for separating polyphosphoinositides on hydroxylapatite.
Journal of Lipid Research, 1990 We describe a method for separation of several phosphoinositides by high performance liquid chromatography (HPLC) for the purposes of identification, analysis, and possible purification of cell phospholipid extracts.
LE Boyle, LA Sklar, AE Traynor-Kaplan
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