Results 11 to 20 of about 10,168 (226)

Cyclic AMP stabilizes the mRNA for phosphoenolpyruvate carboxykinase (GTP) against degradation.

Journal of Biological Chemistry, 1988
It is now well established that cAMP induces the transcription rate of the gene for phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) and that this induction is dependent on a nucleotide domain located within the promoter-regulatory region of the gene (Short, J. M., Wynshaw-Boris, A., Short, H. P., and Hanson, R. W. (1986) J. Biol. Chem.
Y Hod, Richard W. Hanson
semanticscholar   +5 more sources

Overcoming Diabetes-Induced Hyperglycemia through Inhibition of Hepatic Phosphoenolpyruvate Carboxykinase (GTP) with RNAi [PDF]

Molecular Therapy, 2006
Phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) is the rate-controlling enzyme in gluconeogenesis. In diabetic individuals, altered rates of gluconeogenesis are responsible for increased hepatic glucose output and sustained hyperglycemia. Liver-specific inhibition of PEPCK has not been assessed to date as a treatment for diabetes.
Ramon Bartrons, Jordi Bermúdez, Anna Vidal-Alabró, Alicia G. Gómez-Valadés, Jose C. Perales, Maria Molas, Jordi Boada   +6 more
semanticscholar   +5 more sources

Glucocorticoids regulate the induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes

Journal of Biological Chemistry, 1993
The hormonal regulation of transcription of the phosphoenolpyruvate carboxykinase (GTP) (4.1.1.32) (PEPCK) gene during diabetes was studied using transgenic mice containing a chimeric gene consisting of segments of the PEPCK promoter (-2000/+73, -460/+73, -355/+73) linked to bovine growth hormone (bGH) reporter gene.
Richard W. Hanson, Yashomati M. Patel, Jacob E. Friedman, Mary M. McGrane, Jeung S. Yun   +4 more
semanticscholar   +5 more sources

Identification of a DNA clone to phosphoenolpyruvate carboxykinase (GTP) from rat cytosol. Alterations in phosphoenolpyruvate carboxykinase RNA levels detectable by hybridization. [PDF]

Journal of Biological Chemistry, 1981
K Felz, Jonathan Leis, H Yoo-Warren, John Monahan, Michele A. Cimbala, Richard W. Hanson   +5 more
semanticscholar   +4 more sources

Phosphoenolpyruvate Cycling via Mitochondrial Phosphoenolpyruvate Carboxykinase Links Anaplerosis and Mitochondrial GTP with Insulin Secretion [PDF]

Journal of Biological Chemistry, 2009
Pancreatic beta-cells couple the oxidation of glucose to the secretion of insulin. Apart from the canonical K(ATP)-dependent glucose-stimulated insulin secretion (GSIS), there are important K(ATP)-independent mechanisms involving both anaplerosis and mitochondrial GTP (mtGTP).
Michael Roden, Rebecca L. Pongratz, Gerald I. Shulman, Gary W. Cline, Francisco J. Pasquel, Richard G. Kibbey, Romana Stark, Adina Turcu   +7 more
openaire   +5 more sources

A GTP-dependent Vertebrate-type Phosphoenolpyruvate Carboxykinase from Mycobacterium smegmatis [PDF]

Journal of Biological Chemistry, 2001
This is the first report on a bacterial verterbrate-type GTP-dependent phosphoenolpyruvate carboxykinase (PCK). The pck gene of Mycobacterium smegmatis was cloned. The recombinant PCK was overexpressed in Escherichia coli in a soluble form and with high activity. The purified enzyme was found to be monomeric (72 kDa), thermophilic (optimum temperature,
Ralph S. Wolfe, Biswarup Mukhopadhyay, Edward M. Concar   +2 more
openaire   +5 more sources

Nuclear Factor I Regulates Expression of the Gene for Phosphoenolpyruvate Carboxykinase (GTP) [PDF]

Journal of Biological Chemistry, 1998
Nuclear factor-I (NFI) binds to the phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene promoter immediately 5' to the cAMP regulatory element (CRE). This suggests an interaction between NFI and factors that bind the CRE. Of the four NFI isoforms expressed in mammalian tissues, NFI-A and -B stimulate basal transcription from the PEPCK gene promoter in
Ching Y. Hu, Patrick Leahy, Parvin Hakimi, Gregory Grossman, Jason Woods, Deborah R. Crawford, Richard M. Gronostajski, William J. Roesler, Ali Z. Chaudhry, Richard W. Hanson   +9 more
openaire   +4 more sources

Trans regulation of the phosphoenolpyruvate carboxykinase (GTP) gene, identified by deletions in chromosome 7 of the mouse. [PDF]

Proceedings of the National Academy of Sciences, 1986
Livers from newborn mice homozygous for either one of the lethal deletions c14CoS or c3H in chromosome 7 have drastically reduced levels of cytosolic phosphoenolpyruvate carboxykinase (GTP) [GTP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32] activity when compared with normal littermates.
Kenneth S. Krauter, David S. Loose, Salome Gluecksohn-Waelsch, Phyllis A. Shaw, Craig Robinson, Sasha Englard, Richard W. Hanson   +6 more
openaire   +4 more sources

Cysteine 288: An essential hyperreactive thiol of cytosolic phosphoenolpyruvate carboxykinase (GTP)

Journal of Biological Chemistry, 1989
Phosphoenolpyruvate carboxykinase from the cytosol of rat liver has 13 cysteines, at least one of which is known to be very reactive and essential for catalytic activity (Carlson, G. M., Colombo, G., and Lardy, H. A. (1978) Biochemistry 17, 5329-5338). In order to identify the essential cysteine, this enzyme was modified with the fluorescent sulfhydryl
Jerome M. Seyer, C T Lewis, Gerald M. Carlson   +2 more
openaire   +4 more sources

A cAMP-regulated RNA-binding protein that interacts with phosphoenolpyruvate carboxykinase (GTP) mRNA.

Journal of Biological Chemistry, 1993
Cyclic-AMP stabilizes phosphoenolpyruvate carboxykinase (GTP) (PEPCK) mRNA against degradation. To investigate the mechanism of this effect, RNA mobility shift assays were used to determine the interaction of cellular proteins with specific domains from the mRNA.
Yaacov Hod, D Jain, N Nachaliel
openaire   +4 more sources