Results 11 to 20 of about 2,330 (168)

Cysteine 288: An essential hyperreactive thiol of cytosolic phosphoenolpyruvate carboxykinase (GTP)

Journal of Biological Chemistry, 1989
Phosphoenolpyruvate carboxykinase from the cytosol of rat liver has 13 cysteines, at least one of which is known to be very reactive and essential for catalytic activity (Carlson, G. M., Colombo, G., and Lardy, H. A. (1978) Biochemistry 17, 5329-5338). In order to identify the essential cysteine, this enzyme was modified with the fluorescent sulfhydryl
C T, Lewis, J M, Seyer, G M, Carlson
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The gene encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken.

Journal of Biological Chemistry, 1984
The gene for cytosolic phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) from the chicken was isolated from a recombinant library containing the chicken genome in phage lambda Charon 4A. The isolated clone, lambda PCK1cc, contains the complete gene for the enzyme as well as both 5' and 3' flanking sequences. The gene is approximately 8 kilobases in
Y, Hod, H, Yoo-Warren, R W, Hanson
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Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (GTP) from the rat. [PDF]

Proceedings of the National Academy of Sciences, 1983
The gene for cytosolic phosphoenolpyruvate carboxykinase (GTP) [GTP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32] from the rat was isolated from a recombinant library containing the rat genome in phage lambda Charon 4A. The isolated clone, lambda PCK1, contains the complete gene for phosphoenolpyruvate carboxykinase and approximately ...
H, Yoo-Warren, J E, Monahan, J, Short, H, Short, A, Bruzel, A, Wynshaw-Boris, H M, Meisner, D, Samols, R W, Hanson   +8 more
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Glucocorticoids regulate the induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes

Journal of Biological Chemistry, 1993
The hormonal regulation of transcription of the phosphoenolpyruvate carboxykinase (GTP) (4.1.1.32) (PEPCK) gene during diabetes was studied using transgenic mice containing a chimeric gene consisting of segments of the PEPCK promoter (-2000/+73, -460/+73, -355/+73) linked to bovine growth hormone (bGH) reporter gene.
J E, Friedman, J S, Yun, Y M, Patel, M M, McGrane, R W, Hanson   +4 more
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Regulation of phosphoenolpyruvate carboxykinase (GTP) in adipose tissue in vivo by glucocorticoids and insulin [PDF]

Biochemical Journal, 1976
1. The regulation of the synthesis of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) in epididymal adipose tissue, liver and kidney in vivo was studied immunochemically. 2. Phosphoenolpyruvate carboxykinase (GTP) synthesis in adipose tissue is increased by starvation, diabetes and noradrenaline, and decreased by re-feeding and insulin.
O, Meyuhas, L, Reshef, J M, Gunn, R W, Hanson, F J, Ballard   +4 more
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Developmental acquisition of DNase I sensitivity of the phosphoenolpyruvate carboxykinase (GTP) gene in rat liver. [PDF]

Proceedings of the National Academy of Sciences, 1987
The sensitivity to DNase I digestion of the gene encoding rat phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) was assessed during development and prior to the onset of expression. This gene is resistant to DNase I digestion in nuclei isolated from livers of 19-day rat fetuses.
N, Benvenisty, L, Reshef
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Structural mechanisms for cold-adapted activity of phosphoenolpyruvate carboxykinase. [PDF]

Protein Sci
Abstract Temperature is a critical factor in enzyme function, as most enzymes are thermally activated. Across Earth's diverse environments (−20 to 120°C), enzymes have evolved to function optimally at their organism's growth temperature. Thermophilic enzymes must resist denaturation, while psychrophilic enzymes must maintain activity with limited ...
McLeod MJ, Yazdani S, Barwell SAE, Holyoak T.   +3 more
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Disruption of Mitochondrial Dynamics and Integrity Drives Divergent Metabolic Flexibility and Resilience in Podocytes. [PDF]

FASEB J
We investigated two models of mitochondrial dysfunction in podocytes, using two cell culture models: podocytes isolated from mice with Oma1 deletion (Oma1del) and mouse podocytes modified with an inducible knockdown of PHB2 (Phb2kd). OMA1 deficiency induced a glycolytic shift, enhanced glutamine anaplerosis, and sustained energy homeostasis after ...
Özel C, Reitmeier KM, Kieckhöfer E, Abualia K, Nguyen-Minh D, Matin M, Hagmann H, Coward RJM, Brähler S, Antczak P, Schermer B, Benzing T, Giavalisco P, Brinkkoetter PT.   +13 more
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Short-term actions of epigalocatechin-3-gallate in the liver: a mechanistic insight into hypoglycemic and potential toxic effects. [PDF]

FEBS Open Bio
Epigallocatechin‐3‐gallate (EGCG) acutely inhibited gluconeogenesis and enhanced glycolysis, glycogenolysis, and fatty acid oxidation in perfused rat livers. Mechanistic assays revealed mitochondrial uncoupling, inhibition of pyruvate carboxylation and glucose‐6‐phosphatase, shift of NADH/NAD+ ratios toward oxidation, and loss of membrane integrity ...
Bonetti CI, Lopes Correia B, Nakamura Manicardi FC, de Queiroz Eskuarek Melo NM, de Oliveira Pateis V, Comar JF, Babeto de Sá-Nakanishi A, Bracht A, Bracht L.   +8 more
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Biochemical characterization of phosphoenolpyruvate carboxykinases from multiple species of brown algae. [PDF]

J Phycol
Abstract Phosphoenolpyruvate carboxykinase (PEPCK) is involved in the conversion of phosphoenolpyruvate (PEP) to oxaloacetate (OAA). In addition to playing a role in gluconeogenesis in various organisms, PEPCK also functions in the C4 cycle to concentrate CO2 for photosynthesis in some C4 plants.
Jin JQ, Yokooji Y, Shibata T, Atomi H.
europepmc   +2 more sources