Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli
Journal of Hebei University of Science and Technology, 2019 In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show
Xiaoliang HE +6 more
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Metabolic characterisation of E. coli citrate synthase and phosphoenolpyruvate carboxylase mutants in aerobic cultures [PDF]
, 2006 E. coli is still one of the most commonly used hosts for protein production. However, when it is grown with excess glucose, acetate accumulation occurs.
De Maeseneire, Sofie +3 more
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Induction of PEP Carboxylase and Crassulacean Acid Metabolism by Gibberellic Acid in Mesembryanthemum crystallinum [PDF]
, 2001 The induction of Crassulacean acid metabolism in Mesembryanthemum crystallinum was investigated in response to foliar application of gibberellic acid (GA).
Earnest, Jeannine +5 more
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Molecular Plant-Microbe Interactions, 2003 Phosphoenolpyruvate carboxylases (PEPCs), one form of which in each legume species plays a central role in the carbon metabolism in symbiotic root nodules, are activated through phosphorylation of a conserved residue by a specific protein kinase (PEPC-PK)
Tomomi Nakagawa +6 more
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The CreC regulator of Escherichia coli, a new target for metabolic manipulations [PDF]
, 2015 The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism.
Cabrera Gomez, José Gregorio +3 more
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Minimizing acetate formation in E. coli fermentations [PDF]
, 2007 Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product.
De Maeseneire, Sofie +3 more
core +2 more sources
Journal of Plant Interactions, 2022 Karstification forms tremendous karst carbon sinks in the Earth. Whether terrestrial higher plants can absorb and utilize bicarbonate, there is a key testimony that karst carbon sinks can be transformed into carbon sequestrations by terrestrial higher ...
Lei Fang, Yanyou Wu
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Modulation of iron responsive gene expression and enzymatic activities in response to changes of the iron nutritional status in _Cucumis sativus_ L. [PDF]
, 2010 Regulation exerted by the iron status of the plant on the iron deficiency responses was investigated in cucumber roots (_Cucumis sativus_ L.) both at the biochemical and molecular level. Absence of iron induced the expression of the CsFRO1, CsIRT1, CsHA1
Gianpiero Vigani +2 more
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Effect of salicylic acid pretreatment on cadmium toxicity in wheat
Biologia Plantarum, 2010 Cadmium (100, 400 and 1000 μM CdCl2) treatments resulted in the inhibition of root dry biomass, root elongation and increased Cd accumulation in wheat (Triticum aestivum L.) roots.
H. R. Moussa, S. M. El-Gamal
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Evidence of coexistence of C₃ and C₄ photosynthetic pathways in a green-tide-forming alga, Ulva prolifera. [PDF]
PLoS ONE, 2012 Ulva prolifera, a typical green-tide-forming alga, can accumulate a large biomass in a relatively short time period, suggesting that photosynthesis in this organism, particularly its carbon fixation pathway, must be very efficient.
Jianfang Xu +8 more
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