배경: 세포 내 신호전달에 중요한 단백질인 포스포리파제 C-β4(phospholipase C-β4, PLC-β4)의 물리화학적 성질을 연구하기 위해서는 다량의 정제된 단백질이 필요하나 기존의 망막 조직을 이용한 정제 방법은 그 방법이 매우 어려워 충분한 양의 단백질을 정제하는 것이 거의 불가능하다. 따라서 이를 쉽게 정제하기 위하여 배큘로바이러스(baculovirus)를 이용한 발현 및 정제 방법을 개발할 필요가 있다. 또한 배큘로바이러스를 이용한 정제 방법이 확립된 경우 이를 이용하여 키메라 단백질의 발현도 쉽게 할 수 있어 단백질의 성질연구에 매우 유용하다.
openaire +2 more sources
Initiation of atherosclerosis is characterized by accumulation of aggregates of small lipid droplets and vesicles in the extracellular matrix of the arterial intima.
Katariina Öörni+3 more
doaj
Improved rubella hemagglutination inhibtion test: inactivation of non-immunoglobulin hemagglutination inhibitors by phospholipase C [PDF]
Satoru Iwasa, Mayuko Hori
openalex +1 more source
Influence of phospholipase C on some electrical properties of the skeletal muscle membrane
Edson X. Albuquerque, S. Thesleff
openalex +2 more sources
Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells. [PDF]
Ronald M. Burch+2 more
openalex +1 more source
Emerging Roles of Phospholipase C Beta Isozymes as Potential Biomarkers in Cardiac Disorders. [PDF]
Fazio A+11 more
europepmc +1 more source
Phospholipase C zeta: a hidden face of sperm for oocyte activation and early embryonic development. [PDF]
Azil S+4 more
europepmc +2 more sources
Relation of lipid structure of sarcotubular vesicles to Ca++ transport activity
The role of lipids of the sarcotubular membranes in their Ca++ uptake and Mg-ATPase activities was investigated. Treatment of the membranes with phospholipase C inhibits both processes. Treatment with phospholipase A and phospholipase D, which results in
B.P. Yu, F.D. DeMartinis, E.J. Masoro
doaj
Structural basis for the unique molecular properties of broad-range phospholipase C from Listeria monocytogenes. [PDF]
Petrišič N+6 more
europepmc +1 more source