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Phosphoproteome Analysis in Yeast
2003Publisher Summary This chapter describes yeast phosphoproteome analysis using the specific example of the effects of the Reg1p regulatory subunit of Glc7p (PP-1C) on the yeast phosphoproteome. The chapter presents genomics and proteomics linked in order to determine the direct effects of a given gene product.
Rupa, Ray, Timothy A, Haystead
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Phosphoproteomic Techniques and Applications
2014Phosphoproteomic analysis seeks to determine the overall level of protein phosphorylation, as a result of kinase and phosphatase activity, and determine the identity of proteins which are phosphorylated and the amino acid residues which hold the phosphate group.
Ed, Dudley, A Elizabeth, Bond
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Databases for Plant Phosphoproteomics
2015Phosphorylation is the most studied posttranslational modification involved in signal transduction in stress responses, development, and growth. In the recent years large-scale phosphoproteomic studies were carried out using various model plants and several growth and stress conditions.
Waltraud X, Schulze +2 more
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Analysis of Phosphoproteomics Data
2010Regulation of protein phosphorylation plays an important role in many cellular processes, particularly in signal transduction. Diseases such as cancer and inflammation are often linked to aberrant signaling pathways. Mass spectrometry-based methods allow monitoring the phosphorylation status in an unbiased and quantitative manner.
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2020
In contrast to the canonical phosphoproteomes (P-Ser, P-Thr, P-Tyr), the noncanonial phosphoproteomes include phosphorylated side chains not typically acid-stable and thus often missed in standard phosphopeptide mass spectrometry protocols. In this regard the N-phosphohistidinyl residues, the beta aspartyl-phosphate residues, and the S-phosphocysteinyl
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In contrast to the canonical phosphoproteomes (P-Ser, P-Thr, P-Tyr), the noncanonial phosphoproteomes include phosphorylated side chains not typically acid-stable and thus often missed in standard phosphopeptide mass spectrometry protocols. In this regard the N-phosphohistidinyl residues, the beta aspartyl-phosphate residues, and the S-phosphocysteinyl
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Phosphoproteomic Analysis of Peptides
2013Posttranslational modifications represent a major mechanism by which cells control molecular events. Sample preparation is one of the biggest keys in terms of proteomic analysis, and especially when looking at phosphoproteomics. Here we describe two techniques that are used to identify phosphopeptides.
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Enrichment Strategies in Phosphoproteomics
2016The comprehensive study of the phosphoproteome is heavily dependent on appropriate enrichment strategies that are most often, but not exclusively, carried out on the peptide level. In this chapter, I give an overview of the most widely used techniques. In addition to dedicated antibodies, phosphopeptides are enriched by their selective interaction with
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