Results 81 to 90 of about 27,576 (286)

Comprehensive Evaluation of Different TiO₂-Based Phosphopeptide Enrichment and Fractionation Methods for Phosphoproteomics

Cells, 2022
Protein phosphorylation is an essential post-translational modification that regulates multiple cellular processes. Due to their low stoichiometry and ionization efficiency, it is critical to efficiently enrich phosphopeptides for phosphoproteomics ...
Jiaran Li, Jifeng Wang, Yumeng Yan, Na Li, Xiaoqing Qing, Ailikemu Tuerxun, Xiaojing Guo, Xiulan Chen, Fuquan Yang   +8 more
doaj   +1 more source

SLoMo: automated site localization of modifications from ETD/ECD mass spectra [PDF]

, 2009
Recently, software has become available to automate localization of phosphorylation sites from CID data and to assign associated confidence scores. We present an algorithm, SLoMo (Site Localization of Modifications), which extends this capability to ETD ...
Bailey, Christopher M, Cooper, Helen J., Cunningham, Debbie L, Heath, John K, Sweet, Steve M M, Zeller, Martin   +5 more
core   +1 more source

ELABELA Targets Mitochondria to Modulate Heart Development

Advanced Science, EarlyView.
The role of peptide ELABELA (ELA) in cardiomyocyte apoptosis and congenital heart disease (CHD) is unclear. ELA deficiency caused cardiomyocyte apoptosis and CHD. A novel ELA‐APJ‐AKT‐BCL2/BAX axis in regulating mitochondrial function and contributing to CHD pathogenesis was established.
Jian Wang, Qingjie Wang, Zhikang Xu, Shuang Zhou, Yue Zhou, Junjie Yang, Lingfeng Tong, Zhuo Meng, Mei Yang, Wen Zhao, Tie Yang, Hualin Wang, Jun Zhang, Rubin Tan, Lei Wang, Yuqiang Huang, Bin Zhou, Sun Chen, Bing Zhang, Jinxiang Yuan, Jianyuan Zhao, Alex F. Chen, Kun Sun   +22 more
wiley   +1 more source

Quantitative Phosphoproteomic and System-Level Analysis of TOR Inhibition Unravel Distinct Organellar Acclimation in Chlamydomonas reinhardtii

Frontiers in Plant Science, 2018
Rapamycin is an inhibitor of the evolutionary conserved Target of Rapamycin (TOR) kinase which promotes and coordinates translation with cell growth and division.
Valentin Roustan, Wolfram Weckwerth, Wolfram Weckwerth   +2 more
doaj   +1 more source

Proteomic and Phosphoproteomic Profiling Reveals the Oncogenic Role of Protein Kinase D Family Kinases in Cholangiocarcinoma

Cells, 2022
Cholangiocarcinoma (CCA) is a lethal malignancy in the hepatobiliary system, with dysregulated protein expression and phosphorylation signaling. However, the protein and phosphorylation signatures of CCAs are little-known.
Yun Lu, Xiangyu Li, Kai Zhao, Yuanxin Shi, Zhengdong Deng, Wei Yao, Jianming Wang   +6 more
doaj   +1 more source

Noncanonical Amino Acids in the Interrogation of Cellular Protein Synthesis [PDF]

, 2011
Proteins in living cells can be made receptive to bioorthogonal chemistries through metabolic labeling with appropriately designed noncanonical amino acids (ncAAs).
Ngo, John T., Tirrell, David A.
core   +2 more sources

Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation [PDF]

, 2020
Embryonic Stem Cell (ESC) differentiation requires complex cell signalling network dynamics, although the key molecular events remain poorly understood.
Budzyk, Manon, Bustos, Francisco, Fernandez-Alonso, Rosalia, Findlay, Greg M., Helbig, Andreas O., Hukelmann, Jens, Kumar, Pankaj, Lamond, Angus I., Lanner, Fredrik, Petsalaki, Evangelia, Zhou, Houjiang   +10 more
core   +2 more sources

Fibrates Inhibit PLTP‐induced M2 Macrophage Infiltration and Increase the Sensitivity of Hepatocellular Carcinoma to ICIs

Advanced Science, EarlyView.
Phospholipid transfer protein(PLTP) plays a critical role in forming a complex with kinase A (AURKA) and P65. This interaction facilitates phosphorylation of P65 at Ser536, leading to the activation of the NF‐κB signaling pathway. Ultimately, this leads to the upregulation of downstream cytokines, including IL‐6, IL‐8, and CSF‐1, which promotes M2 ...
Xinyue Liang, Yaning Li, Jianxun Cai, Lisi Luo, Pengfei Yang, Yutong Chen, Jiancong Zhou, Yan Zeng, Jiaping Yu, Weiyu Zhang, Wenzheng Pang, Yufang Li, Chunhua Wen, Jian Li, Linjuan Zeng   +14 more
wiley   +1 more source

Quantitative and functional post-translational modification proteomics reveals that TREPH1 plays a role in plant thigmomorphogenesis

, 2018
Plants can sense both intracellular and extracellular mechanical forces and can respond through morphological changes. The signaling components responsible for mechanotransduction of the touch response are largely unknown.
Braam, Janet, Chehab, E. Wassim, Dai, Chen, Li, Ning, Liu, Jun, Liu, Shichang, Qing, Dongjin, Ren, Feng, Wang, Kai, Wu, Madeline, Yang, Zhu, Zhang, Weiping, Zheng, Qingsong   +12 more
core   +2 more sources

R2‐P2 rapid‐robotic phosphoproteomics enables multidimensional cell signaling studies

Molecular Systems Biology, 2019
Recent developments in proteomics have enabled signaling studies where > 10,000 phosphosites can be routinely identified and quantified. Yet, current analyses are limited in throughput, reproducibility, and robustness, hampering experiments that involve ...
Mario Leutert, R. Rodríguez-Mias, Noelle K. Fukuda, J. Villén   +3 more
semanticscholar   +1 more source