Results 91 to 100 of about 27,576 (286)
Rapid Shotgun Phosphoproteomics Analysis
, 2021 In this chapter, we describe a rapid workflow for the shotgun global phosphoproteomics analysis. The strategy is based on the use of accelerated in-solution trypsin digestion under an ultrasonic field by high-intensity focused ultrasound (HIFU) coupled to titanium dioxide (TiO2) selective phosphopeptide enrichment, fractionation by strong cation ...
Carrera, Mónica +2 more
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Advanced Science, EarlyView.Antibody–drug conjugates (ADCs) transform breast cancer therapy, yet resistance limits their durability. Emerging evidence reveals that ADC failure is not solely tumor‐intrinsic but shaped by dynamic tumor–microenvironment interactions that alter drug delivery, processing, and response.
Minji Seo, Jangsoon Lee, Naoto T. Ueno
wiley +1 more source
PhosR enables processing and functional analysis of phosphoproteomic data
Cell Reports, 2021 Summary: Mass spectrometry (MS)-based phosphoproteomics has revolutionized our ability to profile phosphorylation-based signaling in cells and tissues on a global scale.
Hani Jieun Kim +6 more
doaj +1 more source
Advanced Science, EarlyView.This study delineates macrophage heterogeneity along the acute kidney injury to chronic kidney disease transition. Single‐cell RNA sequencing reveals a TRAP5+ scar‐associated macrophage subset driven by Spp1–Cd44 signaling and mitochondrial metabolic reprogramming.
Chenxi Wang +13 more
wiley +1 more source
, 2012 The book provides an overview of state-of-art techniques for the purification, analysis and quantification of proteins in complex samples using different enrichment strategies.
F. Lonardoni, BOSSI, Alessandra Maria
openaire +3 more sources
Advanced Science, EarlyView.In this study, the orange‐muscle giant abalone (Haliotis gigantea) is used as a model to identify a non‐coding SNP that disrupts the interaction between ITGA8 pre‐mRNA and the splicing factor ILF2, leading to altered ITGA8 splicing. These splicing changes promote carotenoid accumulation in abalone muscle through the regulation of tissue remodeling ...
Xiaohui Wei +17 more
wiley +1 more source
Bilirubin as a Modulator of WNK1 Protein Signaling: Implications for Neuroinflammatory Diseases
Advanced Science, EarlyView.ABSTRACT Previously regarded merely as a potentially harmful waste product of heme catabolism, bilirubin has now emerged as a pleiotropic molecule with potent antioxidant, anti‐inflammatory, and hormone‐like properties. Recent findings have revealed protective effects against cardiovascular, metabolic, autoimmune, and neoplastic diseases, as well as ...
Sri Jayanti +3 more
wiley +1 more source
Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways
Bio-ProtocolSignaling pathways are involved in key cellular functions from embryonic development to pathological conditions, with a pivotal role in tissue homeostasis and transformation.
Zilu Ye +2 more
doaj +1 more source
An Efficient Algorithm for Clustering of Large-Scale Mass Spectrometry Data
, 2013 High-throughput spectrometers are capable of producing data sets containing thousands of spectra for a single biological sample. These data sets contain a substantial amount of redundancy from peptides that may get selected multiple times in a LC-MS/MS ...
Hoffert, Jason D. +3 more
core +1 more source