Results 11 to 20 of about 38,220 (259)

Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases

Biomolecules, 2020
Nucleoside phosphorylases catalyze the reversible phosphorolysis of nucleosides to heterocyclic bases, giving α-d-ribose-1-phosphate or α-d-2-deoxyribose-1-phosphate. These enzymes are involved in salvage pathways of nucleoside biosynthesis. The level of
Irina A. Il’icheva, Konstantin M. Polyakov, Sergey N. Mikhailov   +2 more
doaj   +2 more sources

Can Crystal Symmetry and Packing Influence the Active Site Conformation of Homohexameric Purine Nucleoside Phosphorylases?

Croatica Chemica Acta, 2016
It is generaly believed that enzymes retain most of their functionality in the crystal form due to the large solvent content of protein crystals. This is facilitated by the fact that their natural environment in solution is not too far from the one found
Marija Luić, Zoran Štefanić
doaj   +2 more sources

Tri-Cyclic Nucleobase Analogs and Their Ribosides as Substrates of Purine-Nucleoside Phosphorylases. II Guanine and Isoguanine Derivatives

Molecules, 2019
Etheno-derivatives of guanine, O6-methylguanine, and isoguanine were prepared and purified using standard methods. The title compounds were examined as potential substrates of purine-nucleoside phosphorylases from various sources in the reverse ...
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Michał Górka, Agnieszka Bzowska, Beata Wielgus-Kutrowska   +4 more
doaj   +2 more sources

Discovery of two β-1,2-mannoside phosphorylases showing different chain-length specificities from Thermoanaerobacter sp. X-514.

PLoS ONE, 2014
We characterized Teth514_1788 and Teth514_1789, belonging to glycoside hydrolase family 130, from Thermoanaerobacter sp. X-514. These two enzymes catalyzed the synthesis of 1,2-β-oligomannan using β-1,2-mannobiose and d-mannose as the optimal acceptors ...
Kazuhiro Chiku, Takanori Nihira, Erika Suzuki, Mamoru Nishimoto, Motomitsu Kitaoka, Ken'ichi Ohtsubo, Hiroyuki Nakai   +6 more
doaj   +2 more sources

The chemoenzymatic synthesis of clofarabine and related 2′-deoxyfluoroarabinosyl nucleosides: the electronic and stereochemical factors determining substrate recognition by E. coli nucleoside phosphorylases

Beilstein Journal of Organic Chemistry, 2014
Two approaches to the synthesis of 2-chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)adenine (1, clofarabine) were studied. The first approach consists in the chemical synthesis of 2-deoxy-2-fluoro-α-D-arabinofuranose-1-phosphate (12a, 2FAra-1P) via ...
Ilja V. Fateev, Konstantin V. Antonov, Irina D. Konstantinova, Tatyana I. Muravyova, Frank Seela, Roman S. Esipov, Anatoly I. Miroshnikov, Igor A. Mikhailopulo   +7 more
doaj   +2 more sources

Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases. [PDF]

Int J Mol Sci, 2022
Among carbohydrate active enzymes, glycoside phosphorylases (GPs) are valuable catalysts for white biotechnologies, due to their exquisite capacity to efficiently re-modulate oligo- and poly-saccharides, without the need for costly activated sugars as ...
Li A, Benkoulouche M, Ladeveze S, Durand J, Cioci G, Laville E, Potocki-Veronese G.   +6 more
europepmc   +2 more sources

Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof. [PDF]

Angew Chem Int Ed Engl
PsiK from Psilocybe cubensis phosphorylates a diverse set of substituted phenols and benzenediols beyond its native substrate. High‐throughput screening of active site mutant libraries using DESI‐MS enabled protein engineering of PsiK, further expanding its substrate scope.
Kim A, Morato NM, Saha P, Eyimegwu PN, Niyaz AA, Huang R, Cooks RG, Phelan RM, Lewis JC.   +8 more
europepmc   +3 more sources

Diversification of 4ʹ-Methylated Nucleosides by Nucleoside Phosphorylases

ACS Catalysis, 2021
The growing demand for 4'-modified nucleoside analogs in medicinal and biological chemistry is contrasted by the challenging synthetic access to these molecules and the lack of efficient diversification strategies.
Felix Kaspar, M. Seeger, Sarah Westarp, Christoph Köllmann, A. Lehmann, P. Pausch, Sebastian Kemper, P. Neubauer, G. Bange, A. Schallmey, D. Werz, Anke Kurreck   +11 more
semanticscholar   +1 more source

β-Glucan phosphorylases in carbohydrate synthesis

Applied Microbiology and Biotechnology, 2021
β-Glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of β-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of β-glucans as major bioactive compounds. Their preference
Zorica Ubiparip, Marc De Doncker, Koen Beerens, Jorick Franceus, T. Desmet   +4 more
semanticscholar   +1 more source

Side Chain Conformation Restriction in the Catalysis of Glycosidic Bond Formation by Leloir Glycosyltransferases, Glycoside Phosphorylases, and Transglycosidases.

ACS Catalysis, 2021
Carbohydrate side chain conformation is an important factor in the control of reactivity at the anomeric center, ie, in the making and breaking of glycosidic bonds, whether chemically or, for hydrolysis, by glycoside hydrolases. In nature glycosidic bond
Jo Quirke, D. Crich
semanticscholar   +1 more source