Results 61 to 70 of about 868,569 (305)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Facts about Iowa Law Enforcement Physical Standards for Pre-employment Testing, 2011 [PDF]

, 2011
The Iowa Law Enforcement Academy Council, in recognizing the importance of physical fitness status for job performance, established this physical test regimen as a employment standard effective February 15, 1993.

core  

Physically fit or physically literate? Children with special educational needs understanding of physical education [PDF]

, 2011
The role of physical literacy within physical education (PE) has become a widely debated topic in recent years. Its role in educating children about physicality through embodiment, skill acquisition and reading the environment is argued to be of great ...
Alderson P., British Educational Research Association, Cohen L., Crotty M., Department for Education and Skills, Department for Education and Skills, Department for Education and Skills/Qualifications and Curriculum Authority, Giorgi A., Green K., Janine Coates, Killingbeck M., Lee MJ, Mandigo J., NHS, O’Kane C., Penney D., Penney D., Qualifications and Curriculum Authority, Qualifications and Curriculum Authority, Qualifications and Curriculum Development Agency (QCDA), Robson C., Scott J., Smith J., Vickerman P., Whitehead M., Whitehead M., Whitehead M., Woodhead M.   +27 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Loss of popdc3 Impairs Mitochondrial Function and Causes Skeletal Muscle Atrophy and Reduced Swimming Ability in Zebrafish

Journal of Cachexia, Sarcopenia and Muscle
Background The Popeye domain containing 3 (POPDC3) protein is essential for the maintenance of skeletal muscle homeostasis. POPDC3 is a pathogenic variant gene of limb‐girdle muscular dystrophy (LGMD), and its variants lead to LGMDR26.
Chen‐Chen Sun, Zhang‐Lin Chen, Dong Yang, Jiang‐Ling Xiao, Xiang‐Tao Chen, Xi‐Yang Peng, Xiu‐Shan Wu, Chang‐Fa Tang   +7 more
doaj   +1 more source

Measures of the efficacy of a daily fifteen minute fitness programme for intermediate school children : a thesis presented in partial fulfilment of the requirements for the degree of Masterate in Education at Massey University [PDF]

, 1980
This study investigates the effects of a physical fitness training programme on the fitness, reading and mathematics performance of intermediate school pupils.
Lynch, Christopher J
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Motivational and value preferences of townspeople in the field of fitness [PDF]

, 2020
The article presents the results of a survey of Odessa residents as part of a study of the motivational and value preferences of townsfolk in the field of fitness.
Ivanova, Ievgeniia, Shymko, Vitalii, Vystavkina, Daria   +2 more
core  

Developmental change in motor competence : a latent growth curve analysis [PDF]

, 2019
Background: The development of childhood motor competence demonstrates a high degree of inter-individual variation. Some children's competence levels increase whilst others' competence levels remain unchanged or even decrease over time.
Bardid, Farid, Coppens, Eline, D'Hondt, Eva, Deconinck, Frederik, Haerens, Leen, Lenoir, Matthieu, Stodden, David   +6 more
core   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source