Results 91 to 100 of about 16,188 (237)

Combining systems and synthetic biology for in vivo enzymology

The EMBO Journal
Enzymatic parameters are classically determined in vitro, under conditions that are far from those encountered in cells, casting doubt on their physiological relevance. We developed a generic approach combining tools from synthetic and systems biology to
Sara Castaño-Cerezo, Alexandre Chamas, Hanna Kulyk, Christian Treitz, Floriant Bellvert, Andreas Tholey, Virginie Galéote, Carole Camarasa, Stéphanie Heux, Luis F Garcia-Alles, Pierre Millard, Gilles Truan   +11 more
doaj   +1 more source

Strigolactone Levels in Dicot Roots Are Determined by an Ancestral Symbiosis-Regulated Clade of the PHYTOENE SYNTHASE Gene Family

Frontiers in Plant Science, 2018
Strigolactones (SLs) are apocarotenoid phytohormones synthesized from carotenoid precursors. They are produced most abundantly in roots for exudation into the rhizosphere to cope with mineral nutrient starvation through support of root symbionts ...
Ron Stauder, Ralf Welsch, Maurizio Camagna, Wouter Kohlen, Gerd U. Balcke, Alain Tissier, Michael H. Walter   +6 more
doaj   +1 more source

A Natural LTR Retrotransposon Insertion in the Promoter of GhNAC140‐Dt Boosts Cotton Lint Yield

Plant Biotechnology Journal, EarlyView.
ABSTRACT Transposable elements (TEs) are fundamental drivers of crop evolution and domestication. Whereas the underlying mechanisms of TE‐mediated gene activation remain poorly understood. Lint percentage is an important yield component in cotton. Here, we report a retrotransposon insertion in the promoter of GhNAC140‐Dt, a secondary wall NAC encoding ...
Yujia Yu, Xiaoguang Shang, Haitang Wang, Lijie Zhu, Xu Han, Qingfei He, Weixi Li, Yonglin Tan, Guozhong Zhu, Wangzhen Guo   +9 more
wiley   +1 more source

Single genetic locus improvement of iron, zinc and β-carotene content in rice grains

Scientific Reports, 2017
Nearly half of the world’s population obtains its daily calories from rice grains, which lack or have insufficient levels of essential micronutrients. The deficiency of micronutrients vital for normal growth is a global health problem, and iron, zinc and
Simrat Pal Singh, Wilhelm Gruissem, Navreet K. Bhullar   +2 more
doaj   +1 more source

Two Aquaporins Mitigate Growth‐Defence Trade‐Offs by Facilitating CO2 and H2O2 Transport in Wheat

Plant Biotechnology Journal, EarlyView.
ABSTRACT The growth‐defence trade‐offs pose a major challenge to breeding high‐yield and disease‐resistant crops. Aquaporins are membrane channels that facilitate the transport of water and other small compounds, therefore regulating the growth‐defence trade‐offs.
Kai Lu, Shuo Qi, Hui Qian, Xiaohan Fu, Ziyang An, Xiaochen Chen, Liyuan Zhang, Shenshen Zou, Lei Chen, Hansong Dong   +9 more
wiley   +1 more source

A genetic screen for increasing metabolic flux in the isoprenoid pathway of Saccharomyces cerevisiae: Isolation of SPT15 mutants using the screen

Metabolic Engineering Communications, 2016
A genetic screen to identify mutants that can increase flux in the isoprenoid pathway of yeast has been lacking. We describe a carotenoid-based visual screen built with the core carotenogenic enzymes from the red yeast Rhodosporidium toruloides.
M. Wadhwa, A.K. Bachhawat
doaj   +1 more source

Structural characterization of highly glucosylated crocins and regulation of their biosynthesis during flower development in Crocus [PDF]

, 2016
Crocin biosynthesis in Crocus has been proposed to proceed through a zeaxanthin cleavage pathway catalyzed by carotenoid cleavage dioxygenase 2 (CCD2), and followed by glucosylation reactions catalyzed by CsGT2 (UGT74AD1).
Ahrazem, Oussama, Gómez-Gómez, Lourdes, Jimeno, M. Luisa, Rubio-Moraga, Ángela   +3 more
core   +1 more source

Metabolic Reprogramming of a Phenolic Acid by a Plant P450 Monooxygenase Reverses Bacterial Immunosuppression

Plant Biotechnology Journal, EarlyView.
Ralstonia solanacearum suppresses P450 activity to block VA‐to‐VanA conversion, leading to VA accumulation and compromised immunity. ABSTRACT Soil acidification often exacerbates plant diseases caused by soil‐borne pathogens like Ralstonia solanacearum, but the underlying molecular mechanisms remain elusive. This study unveils a sophisticated metabolic
Hua Wang, Xiaoshuang Peng, Xinrui Wang, Ruiyu Yang, Tao Liu, Huidi Liu, Chao Zhao, Hong Zhou, Liang Yang, Shili Li, Wei Ding   +10 more
wiley   +1 more source

cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and ζ-carotene desaturase genes from Solanum lycopersicum KKU-T34003 [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2013
We report on the cloning of Psy1, Pds and Zds cDNAs encoding the enzymes responsible for lycopene biosynthesis,namely phytoene synthase 1 (PSY1), phytoene desaturase (PDS) and -carotene desaturase (ZDS), respectively, from high-lycopene tomato cultivar,
Krittaya Supathaweewat, Preekamol Klanrit   +1 more
doaj  

Engineering Haloferax mediterranei as an Efficient Platform for High Level Production of Lycopene

Frontiers in Microbiology, 2018
Lycopene attracts increasing interests in the pharmaceutical, food, and cosmetic industries due to its anti-oxidative and anti-cancer properties. Compared with other lycopene production methods, such as chemical synthesis or direct extraction from plants,
Zhen-Qiang Zuo, Zhen-Qiang Zuo, Qiong Xue, Qiong Xue, Jian Zhou, Da-He Zhao, Jing Han, Jing Han, Hua Xiang, Hua Xiang   +9 more
doaj   +1 more source