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Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches. [PDF]
Mol HorticWang P +10 more
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cDNA cloning and expression of the phytoene synthase gene in sunflower
Journal of Plant Physiology, 2005A PCR approach was used to isolate a phytoene synthase (Psy) cDNA from sunflower (Helianthus annuus L.). A reconstructed full-length sequence (1598 bp) of the Psy cDNA was obtained; it contained a 1242 bp CDS, 172-nucleotides of 5'-untranslated region (UTR), and 170-nucleotides of 3'-UTR.
Claudio Pugliesi +2 more
exaly +5 more sources
Bioinformatics Analysis and Identification of Phytoene Synthase Gene in Microalgae
Recent Patents on Biotechnology, 2021Background: Carotenoids are known as lipophilic secondary metabolites with important biological activities, which are mostly used in the food and pharmaceutical industry. They contribute to the colours of many fruits and flowers. Studies on the biosynthetic pathways of isoprenoids and carotenoids are still scarce, especially in microalgae and are ...
Saeedeh Shaker +2 more
openaire +2 more sources
Planta, 1982
Envelope membranes of spinach chloroplasts contain appreciable activities of the carotenogenic enzymes phytoene synthase (formation of phytoene by condensation of two molecules geranylgeranyl pyrophosphate) and phytoene dehydrogenase (formation of lycopene from phytoene), plus a phosphatase activity.
Bodo Liedvogel, Hans Kleinig, H Kleinig
exaly +3 more sources
Envelope membranes of spinach chloroplasts contain appreciable activities of the carotenogenic enzymes phytoene synthase (formation of phytoene by condensation of two molecules geranylgeranyl pyrophosphate) and phytoene dehydrogenase (formation of lycopene from phytoene), plus a phosphatase activity.
Bodo Liedvogel, Hans Kleinig, H Kleinig
exaly +3 more sources
Biochemistry, 2003
Phytoene synthase (PSase) catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to phytoene. These reactions constitute the first pathway specific step in carotenoid biosynthesis.
Dirk, Iwata-Reuyl +3 more
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Phytoene synthase (PSase) catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to phytoene. These reactions constitute the first pathway specific step in carotenoid biosynthesis.
Dirk, Iwata-Reuyl +3 more
openaire +2 more sources
Phytoene Accumulation in Sunflower Decreases the Transcript Levels of the Phytoene Synthase Gene
Plant Growth Regulation, 2006The non dormant-1 (nd-1) mutant of sunflower (Helianthus annuus L.) and fluridone-treated plants were used to investigate the effect of phytoene accumulation on the transcript levels of the phytoene synthase (Ha-PSY) gene. A deficiency at the gene coding for ζ-carotene desaturase (ZDS) characterises the nd-1 mutant, which displays an accumulation of ζ ...
L. Campisi +5 more
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Food Chemistry, 2011
Momordica charantia, a tropical plant, produces a fruit that has a β-carotene concentration five times higher than that of carrot. To elucidate the molecular basis of β-carotene accumulation in M. charantia, the gene expression levels of phytoene synthase (McPSY) and phytoene desaturase (McPDS) were determined.
Pham Anh, Tuan +4 more
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Momordica charantia, a tropical plant, produces a fruit that has a β-carotene concentration five times higher than that of carrot. To elucidate the molecular basis of β-carotene accumulation in M. charantia, the gene expression levels of phytoene synthase (McPSY) and phytoene desaturase (McPDS) were determined.
Pham Anh, Tuan +4 more
openaire +2 more sources
Phytochemistry, 1998
Monoclonal antibodies have been prepared against the tomato (Lycopersicon esculentum Mill.) fruit ripening-enhanced phytoene synthase (PSY1). The antigen was prepared as a beta-galactosidase fusion protein by cloning a 1.13 kb fragment of Psy1 cDNA into pUR291, followed by transformation of E. coli.
Peter Bramley +2 more
exaly +3 more sources
Monoclonal antibodies have been prepared against the tomato (Lycopersicon esculentum Mill.) fruit ripening-enhanced phytoene synthase (PSY1). The antigen was prepared as a beta-galactosidase fusion protein by cloning a 1.13 kb fragment of Psy1 cDNA into pUR291, followed by transformation of E. coli.
Peter Bramley +2 more
exaly +3 more sources
Journal of Agricultural and Food Chemistry, 2011
Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan +5 more
openaire +2 more sources
Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan +5 more
openaire +2 more sources