Results 111 to 120 of about 5,798 (146)

Production of phytoene, a carotenoid, and induction of connexin 26 in transgenic mice carrying the phytoene synthase gene crtB

Biochemical and Biophysical Research Communications, 2004
Carotenoids have been recognized as chemopreventive agents against human diseases, such as cancer and cardiovascular disease. Mammalians utilize carotenoids supplied from their food since they are unable to perform the de novo synthesis of carotenoids.
Yoshiko, Satomi, Norihiko, Misawa, Takashi, Maoka, Hoyoku, Nishino   +3 more
openaire   +2 more sources

Transcript expression level analysis of phytoene synthase and phytoene desaturase associated with β-carotene content in selected Kenyan Bitter melon

Molecular Biology Reports, 2022
Bitter melon (Momordica charantia L.) is a widely cultivated food and medicinal plant native to the world's subtropics and tropics. Constraints affecting cultivation of Bitter melon affect productivity of β-carotene. Knowing the mechanism that controls the transcription of the β-carotene biosynthesis genes in Bitter melon will be of great value in ...
Labodé Hospice Naitchede, Aggrey Bernard Nyende, Steven Runo   +2 more
openaire   +2 more sources

Isolation and characterisation of a melon cDNA clone encoding phytoene synthase

Plant Molecular Biology, 1995
A cDNA clone (MEL5), encoding a protein homologous to phytoene synthase (PSY), has been isolated from a climacteric melon fruit cDNA library, using the tomato cDNA clone TOM5 [34] as a heterologous probe. MEL5 hybridised to a transcript of 1.65 kb which suggested that the 1.36 kb clone, isolated originally, was not full-length.
Karvouni, Zoi, John, Isaac, Taylor, Jane E., Watson, Colin F., Turner, Andrew J., Grierson, Don   +5 more
openaire   +2 more sources

Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora

Phytochemistry, 2012
Carotenoid formation was investigated in wild type and carotenogenic mutants of Blakeslea trispora after mating (-) and (+) strains. The highest yields of carotenoids, especially β-carotene was observed following mating. In vitro incorporation of geranylgeranyl pyrophosphate into phytoene and β-carotene corresponded to increased carotenogenesis in the ...
Jürgen, Breitenbach, Paul D, Fraser, Gerhard, Sandmann   +2 more
openaire   +2 more sources

Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro

2019
Phytoene synthase (PSY) is the rate-limiting step in carotenoid biosynthesis, and accordingly subjected to a number of regulatory mechanisms at various levels, including transcriptional, posttranscriptional, and posttranslational. Several PSY genes are present in most taxa and show various degrees of tissue and/or stress-specific responses providing an
Maurizio, Camagna, Ralf, Welsch
openaire   +2 more sources

Structural and functional characterization of the phytoene synthase promoter from Arabidopsis thaliana

Planta, 2003
The expression of the gene coding for the carotenogenic enzyme phytoene synthase is highly regulated. To study this, its promoter and truncated versions thereof were translationally fused to the luciferase gene as a reporter and these constructs were used to transform Arabidopsis thaliana.
Welsch, Ralf, Medina, J., Giuliano, Giovanni, Beyer, Peter, Lintig, Johannes von   +4 more
openaire   +3 more sources

Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene

2010
Carotenoids are well-known natural pigments, typically ranging from yellow to red. Carotenoids are industrially utilized as functional materials due to their strong antioxidant properties. Phytoene synthesis is known to be a rate-determining step in the entire carotenoid biosynthetic pathway in plants.
Masaki, Fujisawa, Norihiko, Misawa
openaire   +2 more sources

Microsatellite polymorphism is likely involved in phytoene synthase activity in Citrus

Plant Cell, Tissue and Organ Culture (PCTOC), 2013
Carotenoid content is an important determinant of pigmentation in citrus fruit. Phytoene synthase (PSY) is an important enzyme in the carotenoid synthesis pathway. In the present study, two similar but non-identical sequences transcripts (CsPsy1a and CsPsy1b) were isolated from sweet orange, and accumulated at a high level throughout the period of ...
Wenfang Zeng, Zongzhou Xie, Xiaoming Yang, Junli Ye, Qiang Xu, Xiuxin Deng   +5 more
openaire   +1 more source

Isolation and expression patterns of a cDNA encoding phytoene synthase in Citrus

Journal of Plant Physiology, 2001
Summary A cDNA clone (Psy1) encoding phytoene synthase was isolated from the fruit cDNA library ofCitrus (Citrus unshiuMarc.). Sequence analyses and phylogenetic dendrogram revealed that the cDNA contains an open reading frame encoding 437 amino acids (47 kDa), which showed significant similarity to those of phytoene synthases of fruit-producing ...
In-Jung Kim, Kyong-Cheol Ko, Chan-Shick Kim, Won-Il Chung   +3 more
openaire   +1 more source

Cloning, expression and characterisation of the cDNA encoding human hepatic squalene synthase, and its relationship to phytoene synthase

Gene, 1993
The reaction catalysed by squalene synthase (SQS) shows many similarities to that performed by another polyisoprene synthase, phytoene synthase (PhS). By identifying sequences conserved between yeast SQS (ySQS) and PhS, we have cloned a 2-kb cDNA (hSQS) encoding human SQS, a protein of 417 amino acids with a predicted M(r) of 48,041, which has only ...
C, Summers, F, Karst, A D, Charles
openaire   +2 more sources