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Isolation and characterisation of a melon cDNA clone encoding phytoene synthase
Plant Molecular Biology, 1995A cDNA clone (MEL5), encoding a protein homologous to phytoene synthase (PSY), has been isolated from a climacteric melon fruit cDNA library, using the tomato cDNA clone TOM5 [34] as a heterologous probe. MEL5 hybridised to a transcript of 1.65 kb which suggested that the 1.36 kb clone, isolated originally, was not full-length.
Karvouni, Zoi +5 more
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Lipids and Lipid Metabolism, 1998
The crtB gene encoding phytoene synthase from the carotenogenic enterobacterium Erwinia uredovora was overexpressed to about 20% of the total cellular protein in Escherichia coli. Formation of the active phytoene synthase had the effect of suppressing the growth of the expressing strain.
Paul Fraser, Gerhard Sandmann
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The crtB gene encoding phytoene synthase from the carotenogenic enterobacterium Erwinia uredovora was overexpressed to about 20% of the total cellular protein in Escherichia coli. Formation of the active phytoene synthase had the effect of suppressing the growth of the expressing strain.
Paul Fraser, Gerhard Sandmann
exaly +3 more sources
Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro
2019Phytoene synthase (PSY) is the rate-limiting step in carotenoid biosynthesis, and accordingly subjected to a number of regulatory mechanisms at various levels, including transcriptional, posttranscriptional, and posttranslational. Several PSY genes are present in most taxa and show various degrees of tissue and/or stress-specific responses providing an
Maurizio, Camagna, Ralf, Welsch
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Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora
Phytochemistry, 2012Carotenoid formation was investigated in wild type and carotenogenic mutants of Blakeslea trispora after mating (-) and (+) strains. The highest yields of carotenoids, especially β-carotene was observed following mating. In vitro incorporation of geranylgeranyl pyrophosphate into phytoene and β-carotene corresponded to increased carotenogenesis in the ...
Jürgen, Breitenbach +2 more
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Planta, 2003
The expression of the gene coding for the carotenogenic enzyme phytoene synthase is highly regulated. To study this, its promoter and truncated versions thereof were translationally fused to the luciferase gene as a reporter and these constructs were used to transform Arabidopsis thaliana.
Welsch, Ralf +4 more
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The expression of the gene coding for the carotenogenic enzyme phytoene synthase is highly regulated. To study this, its promoter and truncated versions thereof were translationally fused to the luciferase gene as a reporter and these constructs were used to transform Arabidopsis thaliana.
Welsch, Ralf +4 more
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Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene
2010Carotenoids are well-known natural pigments, typically ranging from yellow to red. Carotenoids are industrially utilized as functional materials due to their strong antioxidant properties. Phytoene synthesis is known to be a rate-determining step in the entire carotenoid biosynthetic pathway in plants.
Masaki, Fujisawa, Norihiko, Misawa
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Microsatellite polymorphism is likely involved in phytoene synthase activity in Citrus
Plant Cell, Tissue and Organ Culture (PCTOC), 2013Carotenoid content is an important determinant of pigmentation in citrus fruit. Phytoene synthase (PSY) is an important enzyme in the carotenoid synthesis pathway. In the present study, two similar but non-identical sequences transcripts (CsPsy1a and CsPsy1b) were isolated from sweet orange, and accumulated at a high level throughout the period of ...
Wenfang Zeng +5 more
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The y1 gene of maize codes for phytoene synthase.
Genetics, 1996The cloned y1 locus of maize was sequenced and found to encode phytoene synthase. Different "wild-type" alleles of the locus were found to differ by the insertion of transposable elements in their promoter and polyA addition regions, and by the length of a CCA tandem repeat series, without any obvious effect on function of the gene.
B, Buckner +3 more
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Isolation and expression patterns of a cDNA encoding phytoene synthase in Citrus
Journal of Plant Physiology, 2001Summary A cDNA clone (Psy1) encoding phytoene synthase was isolated from the fruit cDNA library ofCitrus (Citrus unshiuMarc.). Sequence analyses and phylogenetic dendrogram revealed that the cDNA contains an open reading frame encoding 437 amino acids (47 kDa), which showed significant similarity to those of phytoene synthases of fruit-producing ...
In-Jung Kim +3 more
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Gene, 1993
The reaction catalysed by squalene synthase (SQS) shows many similarities to that performed by another polyisoprene synthase, phytoene synthase (PhS). By identifying sequences conserved between yeast SQS (ySQS) and PhS, we have cloned a 2-kb cDNA (hSQS) encoding human SQS, a protein of 417 amino acids with a predicted M(r) of 48,041, which has only ...
C, Summers, F, Karst, A D, Charles
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The reaction catalysed by squalene synthase (SQS) shows many similarities to that performed by another polyisoprene synthase, phytoene synthase (PhS). By identifying sequences conserved between yeast SQS (ySQS) and PhS, we have cloned a 2-kb cDNA (hSQS) encoding human SQS, a protein of 417 amino acids with a predicted M(r) of 48,041, which has only ...
C, Summers, F, Karst, A D, Charles
openaire +2 more sources