Results 171 to 180 of about 10,149 (209)

Carotenoid content and expression of phytoene synthase and phytoene desaturase genes in bitter melon (Momordica charantia)

Food Chemistry, 2011
Momordica charantia, a tropical plant, produces a fruit that has a β-carotene concentration five times higher than that of carrot. To elucidate the molecular basis of β-carotene accumulation in M. charantia, the gene expression levels of phytoene synthase (McPSY) and phytoene desaturase (McPDS) were determined.
Pham Anh, Tuan, Jae Kwang, Kim, Nam Il, Park, Sook Young, Lee, Sang Un, Park   +4 more
openaire   +2 more sources

Carotenoid Accumulation and Characterization of cDNAs Encoding Phytoene Synthase and Phytoene Desaturase in Garlic (Allium sativum)

Journal of Agricultural and Food Chemistry, 2011
Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan, Jae Kwang, Kim, Haeng Hoon, Kim, Sook Young, Lee, Nam Il, Park, Sang Un, Park   +5 more
openaire   +2 more sources

cDNA cloning and expression of the phytoene synthase gene in sunflower

Journal of Plant Physiology, 2005
A PCR approach was used to isolate a phytoene synthase (Psy) cDNA from sunflower (Helianthus annuus L.). A reconstructed full-length sequence (1598 bp) of the Psy cDNA was obtained; it contained a 1242 bp CDS, 172-nucleotides of 5'-untranslated region (UTR), and 170-nucleotides of 3'-UTR.
SALVINI, Mariangela, BERNINI A., FAMBRINI M., PUGLIESI C.   +3 more
openaire   +4 more sources

Isolation and characterisation of a melon cDNA clone encoding phytoene synthase

Plant Molecular Biology, 1995
A cDNA clone (MEL5), encoding a protein homologous to phytoene synthase (PSY), has been isolated from a climacteric melon fruit cDNA library, using the tomato cDNA clone TOM5 [34] as a heterologous probe. MEL5 hybridised to a transcript of 1.65 kb which suggested that the 1.36 kb clone, isolated originally, was not full-length.
Karvouni, Zoi, John, Isaac, Taylor, Jane E., Watson, Colin F., Turner, Andrew J., Grierson, Don   +5 more
openaire   +2 more sources

Transcript expression level analysis of phytoene synthase and phytoene desaturase associated with β-carotene content in selected Kenyan Bitter melon

Molecular Biology Reports, 2022
Bitter melon (Momordica charantia L.) is a widely cultivated food and medicinal plant native to the world's subtropics and tropics. Constraints affecting cultivation of Bitter melon affect productivity of β-carotene. Knowing the mechanism that controls the transcription of the β-carotene biosynthesis genes in Bitter melon will be of great value in ...
Labodé Hospice Naitchede, Aggrey Bernard Nyende, Steven Runo   +2 more
openaire   +2 more sources

Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora

Phytochemistry, 2012
Carotenoid formation was investigated in wild type and carotenogenic mutants of Blakeslea trispora after mating (-) and (+) strains. The highest yields of carotenoids, especially β-carotene was observed following mating. In vitro incorporation of geranylgeranyl pyrophosphate into phytoene and β-carotene corresponded to increased carotenogenesis in the ...
Jürgen, Breitenbach, Paul D, Fraser, Gerhard, Sandmann   +2 more
openaire   +2 more sources

Production of phytoene, a carotenoid, and induction of connexin 26 in transgenic mice carrying the phytoene synthase gene crtB

Biochemical and Biophysical Research Communications, 2004
Carotenoids have been recognized as chemopreventive agents against human diseases, such as cancer and cardiovascular disease. Mammalians utilize carotenoids supplied from their food since they are unable to perform the de novo synthesis of carotenoids.
Yoshiko, Satomi, Norihiko, Misawa, Takashi, Maoka, Hoyoku, Nishino   +3 more
openaire   +2 more sources

Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro

2019
Phytoene synthase (PSY) is the rate-limiting step in carotenoid biosynthesis, and accordingly subjected to a number of regulatory mechanisms at various levels, including transcriptional, posttranscriptional, and posttranslational. Several PSY genes are present in most taxa and show various degrees of tissue and/or stress-specific responses providing an
Maurizio, Camagna, Ralf, Welsch
openaire   +2 more sources

Production and characterisation of monoclonal antibodies to phytoene synthase of lycopersicon esculentum

Phytochemistry, 1998
Monoclonal antibodies have been prepared against the tomato (Lycopersicon esculentum Mill.) fruit ripening-enhanced phytoene synthase (PSY1). The antigen was prepared as a beta-galactosidase fusion protein by cloning a 1.13 kb fragment of Psy1 cDNA into pUR291, followed by transformation of E. coli.
P D, Fraser, N, Misawa, G, Sandmann, J, Johnson, W, Schuch, P M, Bramley   +5 more
openaire   +2 more sources

Phytoene synthase from tomato ( Lycopersicon esculentum ) chloroplasts - partial purification and biochemical properties

Planta, 2000
Phytoene synthase activity in tomato chloroplasts is membrane-associated, requiring treatment with high ionic strength buffer or mild non-ionic detergent for solubilisation. Using a combination of ammonium sulphate precipitation, cation and anion exchange, dye-ligand and hydrophobic interaction chromatography, phytoene synthase has been purified 600 ...
P D, Fraser, W, Schuch, P M, Bramley
openaire   +2 more sources