Results 171 to 180 of about 10,172 (200)

Bioinformatics Analysis and Identification of Phytoene Synthase Gene in Microalgae

Recent Patents on Biotechnology, 2021
Background: Carotenoids are known as lipophilic secondary metabolites with important biological activities, which are mostly used in the food and pharmaceutical industry. They contribute to the colours of many fruits and flowers. Studies on the biosynthetic pathways of isoprenoids and carotenoids are still scarce, especially in microalgae and are ...
Saeedeh Shaker, Mohammad Hossein Morowvat, Younes Ghasemi   +2 more
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Bacterial Phytoene Synthase:  Molecular Cloning, Expression, and Characterization ofErwinia herbicolaPhytoene Synthase

Biochemistry, 2003
Phytoene synthase (PSase) catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to phytoene. These reactions constitute the first pathway specific step in carotenoid biosynthesis.
Dirk, Iwata-Reuyl, Shivanand K, Math, Shrivallabh B, Desai, C Dale, Poulter   +3 more
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Phytoene Accumulation in Sunflower Decreases the Transcript Levels of the Phytoene Synthase Gene

Plant Growth Regulation, 2006
The non dormant-1 (nd-1) mutant of sunflower (Helianthus annuus L.) and fluridone-treated plants were used to investigate the effect of phytoene accumulation on the transcript levels of the phytoene synthase (Ha-PSY) gene. A deficiency at the gene coding for ζ-carotene desaturase (ZDS) characterises the nd-1 mutant, which displays an accumulation of ζ ...
L. Campisi, M. Fambrini, V. Michelotti, M. Salvini, D. Giuntini, C. Pugliesi   +5 more
openaire   +2 more sources

Carotenoid Accumulation and Characterization of cDNAs Encoding Phytoene Synthase and Phytoene Desaturase in Garlic (Allium sativum)

Journal of Agricultural and Food Chemistry, 2011
Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan, Jae Kwang, Kim, Haeng Hoon, Kim, Sook Young, Lee, Nam Il, Park, Sang Un, Park   +5 more
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Isolation and characterisation of a melon cDNA clone encoding phytoene synthase

Plant Molecular Biology, 1995
A cDNA clone (MEL5), encoding a protein homologous to phytoene synthase (PSY), has been isolated from a climacteric melon fruit cDNA library, using the tomato cDNA clone TOM5 [34] as a heterologous probe. MEL5 hybridised to a transcript of 1.65 kb which suggested that the 1.36 kb clone, isolated originally, was not full-length.
Karvouni, Zoi, John, Isaac, Taylor, Jane E., Watson, Colin F., Turner, Andrew J., Grierson, Don   +5 more
openaire   +2 more sources

Transcript expression level analysis of phytoene synthase and phytoene desaturase associated with β-carotene content in selected Kenyan Bitter melon

Molecular Biology Reports, 2022
Bitter melon (Momordica charantia L.) is a widely cultivated food and medicinal plant native to the world's subtropics and tropics. Constraints affecting cultivation of Bitter melon affect productivity of β-carotene. Knowing the mechanism that controls the transcription of the β-carotene biosynthesis genes in Bitter melon will be of great value in ...
Labodé Hospice Naitchede, Aggrey Bernard Nyende, Steven Runo   +2 more
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Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora

Phytochemistry, 2012
Carotenoid formation was investigated in wild type and carotenogenic mutants of Blakeslea trispora after mating (-) and (+) strains. The highest yields of carotenoids, especially β-carotene was observed following mating. In vitro incorporation of geranylgeranyl pyrophosphate into phytoene and β-carotene corresponded to increased carotenogenesis in the ...
Jürgen, Breitenbach, Paul D, Fraser, Gerhard, Sandmann   +2 more
openaire   +2 more sources

Production of phytoene, a carotenoid, and induction of connexin 26 in transgenic mice carrying the phytoene synthase gene crtB

Biochemical and Biophysical Research Communications, 2004
Carotenoids have been recognized as chemopreventive agents against human diseases, such as cancer and cardiovascular disease. Mammalians utilize carotenoids supplied from their food since they are unable to perform the de novo synthesis of carotenoids.
Yoshiko, Satomi, Norihiko, Misawa, Takashi, Maoka, Hoyoku, Nishino   +3 more
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Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro

2019
Phytoene synthase (PSY) is the rate-limiting step in carotenoid biosynthesis, and accordingly subjected to a number of regulatory mechanisms at various levels, including transcriptional, posttranscriptional, and posttranslational. Several PSY genes are present in most taxa and show various degrees of tissue and/or stress-specific responses providing an
Maurizio, Camagna, Ralf, Welsch
openaire   +2 more sources

Production and characterisation of monoclonal antibodies to phytoene synthase of lycopersicon esculentum

Phytochemistry, 1998
Monoclonal antibodies have been prepared against the tomato (Lycopersicon esculentum Mill.) fruit ripening-enhanced phytoene synthase (PSY1). The antigen was prepared as a beta-galactosidase fusion protein by cloning a 1.13 kb fragment of Psy1 cDNA into pUR291, followed by transformation of E. coli.
P D, Fraser, N, Misawa, G, Sandmann, J, Johnson, W, Schuch, P M, Bramley   +5 more
openaire   +2 more sources