Results 101 to 110 of about 45,758 (267)

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Microbial Cell Factories, 2008
AbstractBackgroundHigh cell density cultures ofPichia pastorisgrown on methanol tend to develop yellow colored supernatants, attributed to the release of free flavins. The potential ofP. pastorisfor flavin overproduction is therefore given, but not pronounced when the yeast is grown on glucose.
Marx, Hans, Mattanovich, Diethard, Sauer, Michael   +2 more
openaire   +5 more sources

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

Applied Microbiology and Biotechnology, 2014
Pichia pastoris is an established protein expression host mainly applied for the production of biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished production system for its growth to very high cell densities, for the ...
Mudassar Ahmad, Melanie Hirz, H. Pichler, H. Schwab   +3 more
semanticscholar   +1 more source

A High‐Throughput Investigation of the Binding Specificity of Carbohydrate‐Binding Modules for Synthetic and Natural Polymers

ChemSusChem, Volume 18, Issue 16, August 6, 2025.
A high‐throughput screening pipeline is developed to explore the binding specificity of type A carbohydrate‐binding modules (CBMs) to synthetic polymers (polyethylene terephthalate, polystyrene, and polyethylene) and polysaccharides (cellulose, chitin, and starch).
Andrew Philip Rennison, Jaime Fernandez‐Macgregor, Julie Melot, Fabien Durbesson, Tobias Tandrup, Peter Westh, Renaud Vincentelli, Marie Sofie Møller   +7 more
wiley   +1 more source

Enrichment and identification of Δ9-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

Data in Brief, 2015
This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P.
Kerstin Lange, Ansgar Poetsch, Andreas Schmid, Mattijs K. Julsing   +3 more
doaj   +1 more source

Safety Evaluation of Serendipity Berry Sweet Protein From Komagataella phaffii

Journal of Applied Toxicology, Volume 45, Issue 8, Page 1455-1475, August 2025.
ABSTRACT Serendipity Berry Sweet Protein (sweelin) is a novel hyper‐sweet thermophilic protein designed using Artificial Intelligence Computational Protein Design (AI‐CPD) to improve the stability and sensory profile of the protein found in serendipity berry (Dioscoreophyllum cumminsii).
Yael Lifshitz, Shira Paz, Rotem Saban, Inbar Zuker, Hagay Shmuely, Katy Gorshkov, Jwar Meetro, Shahrzad Tafazoli, Trung Vo, Gabriela Amiram, Carmit Shani Levi, Uri Lesmes, Ilan Samish   +12 more
wiley   +1 more source

Expression, purification and application of recombinant Pediocin from Pichia pastoris X33::ped in shrimp preservation trial

Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
Pediocin (Ped) is a 4.6kDa bacteriocin effective against Listeria monocytogenes and several Gram-positive bacteria. To produce purified Ped with high yield and apply it to food preservation, this study expresses and purifies recombinant Ped (rPed) from ...
Nguyễn Phạm Anh Thư, Đặng Thị Phương Thảo, Nguyễn Hiếu Nghĩa   +2 more
doaj   +1 more source

Analyzing the Challenges and Opportunities Associated With Harnessing New Antibiotics From the Fungal Microbiome

MicrobiologyOpen, Volume 14, Issue 4, August 2025.
Graphical abstract illustrating the multifaceted approach to harnessing fungal microbiome diversity for antibiotic discovery. Key elements include historical insights, modern genomic tools, and innovative biotechnological strategies for isolating and enhancing fungal antibiotic production.
Md. Sakhawat Hossain, Md. Al Amin, Sirajul Islam, Hasan Imam, Liton Chandra Das, Shahin Mahmud   +5 more
wiley   +1 more source

Diatom triacylglycerol metabolism: from carbon fixation to lipid droplet degradation

Biological Reviews, Volume 100, Issue 4, Page 1423-1443, August 2025.
ABSTRACT Diatoms are a prominent microalgae family that has attracted interest for its production of molecules of biotechnological interest. Their fatty acid profile is rich in health‐beneficial omega‐3 eicosapentaenoic acid (EPA). Furthermore, under nutrient‐deprived conditions, many diatom species, notably Phaeodactylum tricornutum, Thalassiosira ...
Victor Murison, Josiane Hérault, Justine Marchand, Lionel Ulmann   +3 more
wiley   +1 more source

Engineering protein translocation pathway to improve recombinant proteins in Pichia pastoris

Current Research in Biotechnology
Pichia pastoris is one of the most commonly used hosts for producing heterologous proteins, whereas production levels vary depending on the protein of interest and are also regulated by regulatory factors.
Shengyan Wang, Huijia Dai, Qingling Tang, Yujing Yu, Yaying Xie, Tao Wang, Yide Huang   +6 more
doaj   +1 more source

Anions and citrate inhibit LsAA9A, a lytic polysaccharide monooxygenase (LPMO)

The FEBS Journal, Volume 292, Issue 16, Page 4375-4389, August 2025.
The fungal lytic polysaccharide monooxygenase LsAA9A binds chloride in its crystallization mother liquor, leading to LsAA9A inhibition. Other halides except for fluoride inhibit LsAA9A activity at high concentrations. Transferring crystals to a different solution with lower ionic strength leads to binding of citrate buffer, leading also to the ...
Valerio Di Domenico, Yusuf Theibich, Søren Brander, Jean‐Guy Berrin, Katja S. Johansen, Kristian E. H. Frandsen, Leila Lo Leggio   +6 more
wiley   +1 more source