Economic analysis of royalactin production under uncertainty: Evaluating the effect of parameter optimization. [PDF]
, 2015 Royalactin is a protein with several different potential uses in humans. Research, in insects and in mammalian cells, has shown that it can accelerate cell division and prevent apoptosis.
Aguilar-Yañez, JM +3 more
core +1 more source
Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. [PDF]
PLoS ONE, 2013 The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene.
Hong Hu +9 more
doaj +1 more source
Conformational Transitions Accompanying Oligomerization of Yeast Alcohol Oxidase, a Peroxisomal Flavoenzyme [PDF]
, 1999 Alcohol oxidase (AO) is a homo-octameric flavoenzyme which catalyzes methanol oxidation in methylotrophic yeasts. AO protein is synthesized in the cytosol and subsequently sorted to peroxisomes where the active enzyme is formed.
Boteva, R +6 more
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Species-Specific Differences in the Susceptibility of Fungi to the Antifungal Protein AFP Depend on C-3 Saturation of Glycosylceramides [PDF]
, 2019 AFP is an antimicrobial peptide (AMP) produced by the filamentous fungus Aspergillus giganteus and is a very potent inhibitor of fungal growth that does not affect the viability of bacteria, plant, or mammalian cells.
Baumann, Birgit +8 more
core +1 more source
Preparation of Pichia pastoris Expression Plasmids [PDF]
, 2012 When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a ...
Logez, C +3 more
openaire +4 more sources
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
Synthetic and Systems Biotechnology, 2022 The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) has been extensively engineered for protein production, and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.
Rui Hou +6 more
doaj +1 more source
Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Emberson, Louise M. +3 more
core +1 more source
Genotyping of recombinant Pichia pastoris strains [PDF]
Cellular and Molecular Biology Letters, 2006 AbstractA simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after
Viader Salvadó, José María +3 more
openaire +3 more sources
Trends in Peptide and Protein Sciences, 2017 The use of the methylotrophic yeast, Pichia pastoris, as one of the most effective and versatile systems for the expression of heterologous proteins in biopharmaceutical manufacturing has become increasingly popular in recent years.
Atefeh Namipashaki +3 more
doaj +1 more source
Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1 [PDF]
, 2018 The Starmerella bombicola lactone esterase (SBLE) is a novel enzyme that, in vivo, catalyzes the intramolecular esterification (lactonization) of acidic sophorolipids in an aqueous environment.
Ciesielska, Katarzyna +8 more
core +2 more sources