Results 21 to 30 of about 45,758 (267)
Applied Microbiology and Biotechnology, 2021 Folding and processing of proteins in the endoplasmic reticulum (ER) are major impediments in the production and secretion of proteins from Pichia pastoris (Komagataella sp.).
Hana Raschmanová +4 more
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Comparative genomics and transcriptomics of Pichia pastoris [PDF]
BMC Genomics, 2016 Pichia pastoris has emerged as an important alternative host for producing recombinant biopharmaceuticals, owing to its high cultivation density, low host cell protein burden, and the development of strains with humanized glycosylation. Despite its demonstrated utility, relatively little strain engineering has been performed to improve Pichia, due in ...
Sarah Young +11 more
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Microbial Cell Factories, 2020 Porcine growth hormone (pGH) is a class of peptide hormones secreted from the pituitary gland, which can significantly improve growth and feed utilization of pigs. However, it is unstable and volatile in vitro.
Jinbo Deng +12 more
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Journal of Cellular Physiology, 2020 One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts,
M. Karbalaei, S. Rezaee, Hadi Farsiani
semanticscholar +1 more source
Autolysis of Pichia pastoris induced by cold [PDF]
AMB Express, 2017 The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins.
Yaneth Bartolo-Aguilar +6 more
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Sensors, 2023 This paper introduces a novel soft sensor modeling method based on BDA-IPSO-LSSVM designed to address the issue of model failure caused by varying fermentation data distributions resulting from different operating conditions during the fermentation of ...
Bo Wang, Jun Liu, Ameng Yu, Haibo Wang
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Cloning and Expression of Yak Active Chymosin in [PDF]
Asian-Australasian Journal of Animal Sciences, 2016 Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production ...
Fan Luo +4 more
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High-yield overproduction and purification of human aquaporins from Pichia pastoris
STAR Protocols, 2022 Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia
Tamim Al-Jubair +7 more
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FEMS Yeast Research, 2021 Pichia pastoris is one of the most widely used host for the production of recombinant proteins. Expression systems that rely mostly on promoters from genes encoding alcohol oxidase 1 or glyceraldehyde-3-phosphate dehydrogenase have been developed ...
Burcu Gündüz Ergün +3 more
semanticscholar +1 more source
BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
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