Functional Antibody-Dependent Enhancement as an Immune Assessment Platform: Development, Standardization, and Translational Interpretation in Flavivirus Research. [PDF]
Moi ML.
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Evolutionary patterns of pH1N1 and H3N2 in relation to vaccine use. [PDF]
Lin YW +8 more
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Evaluation of protective efficacy of an inactivated Japanese encephalitis virus genotype Ⅴ strain in mice. [PDF]
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Optimally fixed F protein on the surface of RSV-infected cells for RSV binding and neutralizing assays. [PDF]
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Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test
Methods in Molecular Biology, 2022Neutralization assays are often used as part of research and diagnostics to detect neutralizing antibodies and to determine a possible protective antibody titer after infection or vaccination. Here we describe a conventional plaque reduction neutralization test (PRNT) to check the presence of antibodies against SARS-CoV-2 in patient samples (serum or ...
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Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
Methods in Molecular Biology, 2016Neutralization assay is a technique that detects and quantifies neutralizing antibody in serum samples by calculating the percentage of reduction of virus activity, as the concentration of virus used is usually constant. Neutralizing antibody titer is conventionally determined by calculating the percentage reduction in total virus infectivity by ...
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Review of diagnostic plaque reduction neutralization tests for flavivirus infection
Veterinary Journal, 2013Flavivirus infections (including Japanese encephalitis, West Nile encephalitis and dengue fever/severe dengue) present a worldwide public health problem. Recent climate change may affect the geographical distribution of the arthropod vectors for these viruses and so the risk of flavivirus epidemics may increase. Many methods have been developed for the
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Antigenic Relationships of Group a Arboviruses by Plaque Reduction Neutralization Testing
American Journal of Tropical Medicine and Hygiene, 1975Antigenic relationships of 20 group A arboviruses were assessed by the plaque reduction neutralization test, using highly specific hyperimmune mouse ascitic fluids and antisera. The existence of three complexes of group A viruses was verified. With rare exceptions, heterologous neutralization reactions were observed only among viruses in the same ...
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Accuracy and repeatability of a micro plaque reduction neutralization test for vaccinia antibodies
Biologicals, 2008The detection of neutralizing antibodies against vaccinia virus is a valuable tool for the investigation of previous smallpox vaccination. Compulsory smallpox vaccination ended in Brazil during the early 1970s, although the vaccine was available until the late 1970s.
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Foot-and-mouth disease virus: Plaque reduction neutralization test
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