Results 191 to 200 of about 16,802 (217)
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A Plaque Reduction Test for Dengue Virus Neutralizing Antibodies
The Journal of Immunology, 1967Summary A technique for performing a plaque reduction test for dengue virus neutralizing antibody is described. Experiments designed to test the accuracy of the antibody measurement are presented and analysed by the method for a parallel line graded response assay.
P K, Russell +3 more
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Dengue Virus Identification by the Plaque Reduction Neutralization Test
The Journal of Immunology, 1967Summary A method for identification and serologic classification of dengue viruses using a plaque reduction neutralization test and monkey immune serum is described. Results of typing 40 strains confirm the usefulness of this method for classification of low passage strains.
P K, Russell, A, Nisalak
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A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies
2020Neutralizing antibodies against Zika virus (ZIKV) are a robust indicator of exposure to ZIKV. Here we describe the plaque reduction neutralization test (PRNT), which quantifies ZIKV-neutralizing antibodies (nAbs) by incubating infectious ZIKV with different dilutions of sera and applying the mixture to a susceptible Vero cell monolayer. After 4 days of
Kristina, Dimitrova +3 more
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Neutralization test for bk virus: plaque reduction detected by immunoperoxidase staining
Journal of Medical Virology, 1986AbstractWe developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzymelinked immunosorbent assay ...
T, Flaegstad +3 more
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Guidelines for Plaque-Reduction Neutralization Testing of Human Antibodies to Dengue Viruses
Viral Immunology, 2008Through the Advisory Committee on Dengue and other Flavivirus Vaccines, the World Health Organization(WHO) has had a long-standing commitment to facilitate and to guide research and development of vaccines for medically important flaviviruses. Recently, the Paediatric Dengue Vaccine Initiative (PDVI) was formed to accelerate the development, testing ...
John T, Roehrig +2 more
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A Study of the Neutralization of Rhinoviruses by Means of a Plaque-Reduction Test
The Journal of Immunology, 1969Abstract Neutralizing antibodies to four rhinoviruses were measured by the reduction in the number of plaque-forming units of virus by antiserum. Both the 50% plaque-reduction titer and the neutralization constant, K, were used to characterize reproducibly the neutralizing activity of an antiserum.
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Virology, 1968
Abstract Antisera to several different cytoplasmic frog virus isolates were prepared in rabbits. Comparison of eight virus isolates by means of 50% plaque reduction tests revealed no antigenic differences. In neutralization kinetic studies of three isolates, LT-1, LT-2, and FV-1, very low neutralization rate constants (K = 0.18–2.9) and high ...
D E, Lehane, H F, Clark, D T, Karzon
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Abstract Antisera to several different cytoplasmic frog virus isolates were prepared in rabbits. Comparison of eight virus isolates by means of 50% plaque reduction tests revealed no antigenic differences. In neutralization kinetic studies of three isolates, LT-1, LT-2, and FV-1, very low neutralization rate constants (K = 0.18–2.9) and high ...
D E, Lehane, H F, Clark, D T, Karzon
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Biologicals
Measles is an infectious febrile sickness caused by the measles virus (MeV). Despite an effective vaccine, regional elimination of measles remains a global priority and still faces challenges. To estimate community protection against measles, sensitive tests are needed to identify measles-specific antibodies.
Somayeh, Yaghoobizad +9 more
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Measles is an infectious febrile sickness caused by the measles virus (MeV). Despite an effective vaccine, regional elimination of measles remains a global priority and still faces challenges. To estimate community protection against measles, sensitive tests are needed to identify measles-specific antibodies.
Somayeh, Yaghoobizad +9 more
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The Thai Journal of Pharmaceutical Sciences, 2022
Introduction and objective: Since 2020, little is known about neutralizing antibody (NAb) responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus emerging and causing COVID-19 in humans. Here, we established a plaque reduction neutralization test (PRNT) to use as a method for detecting functional NAbs to SARS-CoV ...
Supaporn Phumiamorn +6 more
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Introduction and objective: Since 2020, little is known about neutralizing antibody (NAb) responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus emerging and causing COVID-19 in humans. Here, we established a plaque reduction neutralization test (PRNT) to use as a method for detecting functional NAbs to SARS-CoV ...
Supaporn Phumiamorn +6 more
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Bluetongue Virus: (1) In Pregnant White-tailed Deer (2) A Plaque Reduction Neutralization Test
Journal of Wildlife Diseases, 1970Six white-tailed deer (Odocoileus virginianus) were infected with bluetongue virus (BTs, vaccinal strain) approximately one-third of the way through their gestation period. One deer died of bluetongue 21 days after inoculation. Of the five surviving the infection, one had two mummified fetuses, and the others no fetuses upon euthanasia two weeks after ...
F C, Thomas, D O, Trainer
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