Results 11 to 20 of about 741,058 (313)
Microbiology Spectrum, 2014 ABSTRACT The stable maintenance of low-copy-number plasmids in bacteria is actively driven by partition mechanisms that are responsible for the positioning of plasmids inside the cell. Partition systems are ubiquitous in the microbial world and are encoded by many bacterial chromosomes as well as plasmids. These systems, although different in
Jamie C, Baxter, Barbara E, Funnell
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ParB Partition Proteins: Complex Formation and Spreading at Bacterial and Plasmid Centromeres
Frontiers in Molecular Biosciences, 2016 In bacteria, active partition systems contribute to the faithful segregation of both chromosomes and low-copy-number plasmids. Each system depends on a site-specific DNA binding protein to recognize and assemble a partition complex at a centromere-like ...
Barbara Funnell
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Stoichiometry of P1 Plasmid Partition Complexes [PDF]
Journal of Biological Chemistry, 2000 The P1 plasmid prophage is faithfully partitioned by a high affinity nucleoprotein complex assembled at the centromere-like parS site. This partition complex is composed of P1 ParB and Escherichia coli integration host factor (IHF), bound specifically to parS. We have investigated the assembly of ParB at parS and its stoichiometry of binding.
J Y, Bouet, J A, Surtees, B E, Funnell
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Nonspecific DNA binding by P1 ParA determines the distribution of plasmid partition and repressor activities. [PDF]
J Biol Chem, 2020 The faithful segregation, or “partition,” of many low-copy number bacterial plasmids is driven by plasmid-encoded ATPases that are represented by the P1 plasmid ParA protein.
Baxter JC, Waples WG, Funnell BE.
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Fine-structure analysis of the P1 plasmid partition site [PDF]
Journal of Bacteriology, 1991 P1 plasmid partition requires two plasmid-encoded Par proteins and a cis-acting site. The site, parS, lies in a region consisting of a 13-bp palindrome and an adjacent AT-rich sequence. A series of point mutations were analyzed for their effects on partition site activity. The results indicated that only the left arm of the palindrome and some adjacent
K A, Martin, M A, Davis, S, Austin
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Plasmid partition and incompatibility – the focus shifts [PDF]
Molecular Microbiology, 2007 SummaryThe mitotic apparatus that a plasmid uses to ensure its stable inheritance responds to the appearance of an additional copy of the plasmid's centromere by segregating it from the pre‐existing copies: if the new copy arises by replication of the plasmid the result is partition, if it arrives on a different plasmid the result is incompatibility ...
Jean-Yves, Bouet +2 more
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Pairing of P1 plasmid partition sites by ParB [PDF]
Molecular Microbiology, 2001 The mechanisms by which bacterial plasmids and chromosomes are partitioned are largely obscure, but it has long been assumed that the molecules to be separated are initially paired, as are sister chromatids in mitosis. We offer in vivo evidence that the partition protein ParB encoded by the bacterial plasmid P1 can pair cis‐acting partition sites of P1
R, Edgar, D K, Chattoraj, M, Yarmolinsky
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ParA-mediated plasmid partition driven by protein pattern self-organization. [PDF]
EMBO J, 2013 Hwang LC +6 more
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Bacterial plasmid partition machinery: a minimalist approach to survival. [PDF]
Curr Opin Struct Biol, 2012 Schumacher MA.
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Dissection of the ATPase active site of P1 ParA reveals multiple active forms essential for plasmid partition. [PDF]
J Biol Chem, 2013 Background: The ParA partition ATPase drives the dynamic positioning of plasmids in bacteria. Results: Different mutations in a critical lysine of the P1 ParA ATP binding site block different steps in partition.
Vecchiarelli AG +5 more
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