Results 131 to 140 of about 627 (174)

Inactivation of Human Serum Plasminogen Antiactivator by Synthetic Fibrinolysis Inducers

Thrombosis and Haemostasis, 1969
SummaryHuman serum plasminogen antiactivator was separated from serum antiplasmin by isoelectric precipitation and ammonium sulfate fractionation followed by Sephadex chromatography. With the use of human vascular activator it was demonstrated that the serum antiactivator is functionally distinct from serum antiplasmin.
N, Aoki, K N, von Kaulla
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Fibrin Targeting of Echogenic Liposomes with Inactivated Tissue Plasminogen Activator

Journal of Liposome Research, 2008
Fibrin-specific molecular targeting strategies are desirable for site-specific imaging and treatment of late stage atheroma, but fibrin-specific antibodies are difficult to produce and present immunogenicity problems. Tissue plasminogen activator (tPA) is an endogenous protein that has been shown to bind fibrin with high affinity and may circumvent ...
Melvin E, Klegerman, Yuejiao, Zou, David D, McPherson   +2 more
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A cyclopeptidic suicide substrate preferentially inactivates urokinase-type plasminogen activator

Biochemical and Biophysical Research Communications, 1991
c[Arg-aB-(CH2+SCH3 phi)-Gly4] was designed and studied as a mechanism-based inactivator (suicide substrate) for plasminogen activators (u-PA and t-PA) and plasmin. This compound inhibited u-PA and fulfills criteria expected for the involvement of an enzyme-activated inhibitor: first-order and irreversible process, saturation kinetics, protection by ...
M, Reboud-Ravaux, A C, Vilain, N, Boggetto, J, Maillard, C, Favreau, J, Xie, J P, Mazaleyrat, M, Wakselman   +7 more
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Heat Inactivation of Native Plasminogen Activators in Bovine Milk

Journal of Food Science, 1993
ABSTRACT Inactivation of plasminogen activators appeared to follow first‐order kinetics in the temperature range tested. The Arrhenius plot was linear (correlation coefficient 0.990). The Arrhenius equation for heat inactivation was deduced, from which the rate of inactivation at any temperature in the range 60–140°C could be ...
DONGJIN D. LU, S. SUZANNE NIELSEN
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Biological Effects of Combined Inactivation of Plasminogen Activator and Plasminogen Activator Inhibitor-1 Gene Function in Mice

Thrombosis and Haemostasis, 1995
SummaryMice with combined homozygous deficiency of tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA) (T‾U‾), of t-PA and plasminogen activator inhibitor-1 (PAI-1) (T‾P‾), of u-PA and PAI-1 (U‾P‾) or of t-PA, u-PA, and PAI-1 (T‾U‾P‾) were generated by inbreeding of mice with the respective deficiencies.
H R, Lijnen, L, Moons, V, Beelen, P, Carmelie, D, Collen   +4 more
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Human Plasminogen Is Highly Susceptible to Peroxynitrite Inactivation

Clinical Chemistry and Laboratory Medicine, 2003
It has been shown that diabetic patients have up to three-fold increases in plasma nitrated tyrosine. We hypothesize that nitration of plasminogen could impair its catalytic properties and be a factor in diabetic thrombogenicity. To test this hypothesis, in this study we addressed the effects of the peroxynitrite donor 3-morpholinosydnonimine (SIN-1 ...
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Inactivation of Plasminogen Activator Inhibitor-1 by RNA Aptamer Molecules

Blood, 2012
Abstract Abstract 1107 Introduction: The serine protease inhibitor (serpin) plasminogen activator inhibitor-1 (PAI-1), binds and inhibits the following plasminogen activators: tissue-type plasminogen activator (tPA), and urokinase-type plasminogen activator (uPA). This decreases plasmin
Yolanda Fortenberry, Jared Damare
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Inactivation of single-chain urokinase-type plasminogen activator by thrombin in human subjects

Journal of Laboratory and Clinical Medicine, 1999
Thrombin cleaves single-chain urokinase-type plasminogen activator (scu-PA) into a virtually inactive two-chain form (tcu-PA/T), a process that may protect a blood clot from early fibrinolysis. It is not known under what circumstances tcu-PA/T can be generated in vivo.
Braat, E.A.M., Levi, M., Bos, R., Haverkate, F., Lassen, M.R., Maat, M.P.M. de, Rijken, D.C.   +6 more
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Effect of Peptides on the Inactivation of Tissue Plasminogen Activator by Plasminogen Activator Inhibitor-1 and on the Binding of Tissue Plasminogen Activator to Endothelial Cells

Thrombosis and Haemostasis, 1990
SummaryThe effectiveness of tissue plasminogen activator (tPA) in thrombolytic therapy is dependent upon the rate at which therapeutically administered tPA reaches the clot site and the proportion of that tPA which is enzymatically active. Interactions between tPA and its main plasma inhibitor (PAI-1) and between tPA and the endothelial cells lining ...
A J, Wittwer, M A, Sanzo
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Inactivation of the plasminogen activator from HeLa cells by peptides of arginine chloromethyl ketone

Biochimica et Biophysica Acta (BBA) - Enzymology, 1979
The binding specificities of human urinary urokinase (EC 3.4.99.26) and HeLa cell plasminogen activator were studied using peptidyl chloromethyl ketone inhibitors. A 125I-labeled fibrin assay has been developed to yield kinetic information. Reagents of the sequence X-Gly-ArgCH2Cl were the most effective.
P, Coleman, C, Kettner, E, Shaw
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