Results 141 to 150 of about 627 (174)
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Acid treatment of plasma for the inactivation of plasminogen activator inhibitor-1 (PAI-1)
Thrombosis Research, 1988The present study was initiated to assess the effectiveness of various acid treatments of blood or plasma in the inactivation of PAI-1. It was shown that a frequently used treatment of blood or plasma with 1 M acetate buffer, pH 3.9, only partially inactivated PAI-1.
M P, de Maat +4 more
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Circulation, 1995
Background Despite the high frequency of pulmonary thromboembolism and its significant morbidity and mortality, diagnosis remains suboptimal. We have been developing a method for prompt detection with the use of radiolabeled, inactivated tissue-type plasminogen activator (TPA) and performed the present study to determine ...
V H, De Bruyn +3 more
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Background Despite the high frequency of pulmonary thromboembolism and its significant morbidity and mortality, diagnosis remains suboptimal. We have been developing a method for prompt detection with the use of radiolabeled, inactivated tissue-type plasminogen activator (TPA) and performed the present study to determine ...
V H, De Bruyn +3 more
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Urokinase Activation of Plasminogen and Spontaneous Inactivation of the Plasmin Formed
Thrombosis and Haemostasis, 1968SummaryThe kinetics of the activation of plasminogen into plasmin with urokinase and the inactivation rate of the plasmin formed are studied.As a first order reaction is obtained with low plasminogen concentrations and a zero-order reaction is obtained with high concentrations, the activation seems to follow the Michaelis-Menten’s law.
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Thrombosis and Haemostasis, 1981
Besides active plasminogen activators, the synthesis of a proactivator (preurokinase) has been reported in cell and tissue cultures. It is suggested that slow activation of the proactivator in the culture medium occurs by an unknown proteolytic mechanism.
G Wijngaards, M B Bernik
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Besides active plasminogen activators, the synthesis of a proactivator (preurokinase) has been reported in cell and tissue cultures. It is suggested that slow activation of the proactivator in the culture medium occurs by an unknown proteolytic mechanism.
G Wijngaards, M B Bernik
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Thrombosis and Haemostasis, 1998
SummaryTo investigate a potential physiological role of the plasminogen/ plasmin system in activation of the matrix metalloproteinase (MMP) system, the distribution of latent and active MMP-2 (gelatinase A) or MMP-9 (gelatinase B) was monitored in aorta extracts and in serum-free conditioned cell culture medium obtained from wild-type (WT) mice and ...
H R, Lijnen +4 more
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SummaryTo investigate a potential physiological role of the plasminogen/ plasmin system in activation of the matrix metalloproteinase (MMP) system, the distribution of latent and active MMP-2 (gelatinase A) or MMP-9 (gelatinase B) was monitored in aorta extracts and in serum-free conditioned cell culture medium obtained from wild-type (WT) mice and ...
H R, Lijnen +4 more
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Fibrinolysis, 1991
Abstract Tissue plasminogen activator (t-PA) is synthesised as a one chain molecule which can be converted to a two chain form by hydrolysis of the peptide bond after the arginine at position 275 by plasmin. Variants with non-basic residues at position 275 are resistant to this hydrolysis. However, incubation of these variants with plasmin can result
D.L. Higgins, S.L. Young, A. Wong
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Abstract Tissue plasminogen activator (t-PA) is synthesised as a one chain molecule which can be converted to a two chain form by hydrolysis of the peptide bond after the arginine at position 275 by plasmin. Variants with non-basic residues at position 275 are resistant to this hydrolysis. However, incubation of these variants with plasmin can result
D.L. Higgins, S.L. Young, A. Wong
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Thrombosis Research, 1987
Two-chain tissue plasminogen activator (t-PA) was found to be inactive in a coupled colorimetric assay for plasminogen activators, but a high level of activity was obtained in the presence of poly-D-lysine. This stimulated activity was strongly inhibited by minactivin, a urokinase inhibitor, but unstimulated enzyme could be shown to be unaffected by ...
K C, Leung, J A, Byatt, R W, Stephens
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Two-chain tissue plasminogen activator (t-PA) was found to be inactive in a coupled colorimetric assay for plasminogen activators, but a high level of activity was obtained in the presence of poly-D-lysine. This stimulated activity was strongly inhibited by minactivin, a urokinase inhibitor, but unstimulated enzyme could be shown to be unaffected by ...
K C, Leung, J A, Byatt, R W, Stephens
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Thrombosis and Haemostasis, 1997
SummaryPlasminogen-dependent and -independent proteolytic activity of murine endothelioma (End) cells that were derived from mice with targeted inactivation of the tissue-type plasminogen activator (t-PA -/-), urokinase-type plasminogen activator (u-PA-/-) or plasminogen activator inhibitor-1 (PAI-1 -/- genes was studied with the use of fibrin and ...
H R, Lijnen, E F, Wagner, D, Collen
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SummaryPlasminogen-dependent and -independent proteolytic activity of murine endothelioma (End) cells that were derived from mice with targeted inactivation of the tissue-type plasminogen activator (t-PA -/-), urokinase-type plasminogen activator (u-PA-/-) or plasminogen activator inhibitor-1 (PAI-1 -/- genes was studied with the use of fibrin and ...
H R, Lijnen, E F, Wagner, D, Collen
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Clinical Chemistry and Laboratory Medicine, 2008
Myeloperoxidase (MPO) neutrophils have been considered an important pathophysiological factor in oxidative stress. Mainly through generation of hypochlorous acid in the phagosome, unchecked activity may lead to inactivation of important proteins through modification of tyrosine and other residues.
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Myeloperoxidase (MPO) neutrophils have been considered an important pathophysiological factor in oxidative stress. Mainly through generation of hypochlorous acid in the phagosome, unchecked activity may lead to inactivation of important proteins through modification of tyrosine and other residues.
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
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