Results 111 to 120 of about 212,295 (303)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

The Replication Argument for Incompatibilism [PDF]

, 2019
In this paper, I articulate an argument for incompatibilism about moral responsibility and determinism. My argument comes in the form of an extended story, modeled loosely on Peter van Inwagen’s “rollback argument” scenario.
A Mele, B Matheson, CE Franklin, D Pereboom, E Barnes, G Watson, JM Fischer, K Demetriou, M McKenna, ME Schlosser, N Tognazzini, P Inwagen van, P Russell, P Todd, P Todd, P Todd, P Todd, P Todd, Patrick Todd, R Waller, S Smilansky   +20 more
core   +2 more sources

Variability in the Content of Phenolic Compounds in Plum Fruit

Plants, 2020
The aim of this study was to determine the composition and content of phenolic compounds in extracts of plum fruit. Fruit of 17 plum cultivars were analyzed. Fruit samples were collected in 2019 from fruit trees with “Myrobalan” (P. cerasifera Ehrh.) and
M. Liaudanskas, Rugilė Okulevičiūtė, J. Lanauskas, D. Kviklys, K. Zymonė, T. Rendyuk, V. Žvikas, N. Uselis, V. Janulis   +8 more
semanticscholar   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

BMC Biotechnology, 2017
Background The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity.
Michael Puckette, Thomas Burrage, John G. Neilan, Max Rasmussen   +3 more
doaj   +1 more source

Primi studi sulla biologia e sul controllo di Cydia funebrana (Treitschke) in susineti biologici siciliani [PDF]

, 2011
First studies on biology and control of Cydia funebrana (treitschke) in Sicilian organic plum orchards. Cydia funebrana (Treitschke), considered the key pest in the Sicilian plum orchards. Few studies were carried out in Sicily concerning C.
LO VERDE, Gabriella, RIZZO, Roberto
core  

Strong screening in the plum pudding model

, 2011
We study a generalized Thomson problem that appears in several condensed matter settings: identical point-charge particles can penetrate inside a homogeneously charged sphere, with global electro-neutrality.
Chepelianskii, A. D., Closa, F., Raphael, E., Trizac, E.   +3 more
core   +3 more sources

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source