Results 41 to 50 of about 184,232 (306)

A Highly Effective African Swine Fever Virus Vaccine Elicits a Memory T Cell Response in Vaccinated Swine

Pathogens, 2022
African Swine Fever Virus (ASFV) is the causative agent of a highly contagious and lethal vector-borne disease in suids. Recently, a live attenuated virus strain, developed using the currently circulating, virulent Georgia strain (ASFV-G) with a single ...
Sarah E. Attreed, Christina Silva, Sophia Abbott, Elizabeth Ramirez-Medina, Nallely Espinoza, Manuel V. Borca, Douglas P. Gladue, Fayna Diaz-San Segundo   +7 more
doaj   +1 more source

Localized Modes of the Linear Periodic Schr\"{o}dinger Operator with a Nonlocal Perturbation [PDF]

, 2008
We consider the existence of localized modes corresponding to eigenvalues of the periodic Schr\"{o}dinger operator $-\partial_x^2+ V(x)$ with an interface. The interface is modeled by a jump either in the value or the derivative of $V(x)$ and, in general,
Dohnal, Tomáš, Plum, Michael, Reichel, Wolfgang   +2 more
core   +3 more sources

Effect of Foot-and-Mouth Disease Virus 2B Viroporin on Expression and Extraction of Mammalian Cell Culture Produced Foot-and-Mouth Disease Virus-like Particles

Vaccines, 2022
To improve the production of foot-and-mouth disease (FMD) molecular vaccines, we sought to understand the effects of the FMD virus (FMDV) 2B viroporin in an experimental, plasmid-based, virus-like particle (VLP) vaccine.
Victoria Primavera, Janine Simmons, Benjamin A. Clark, John G. Neilan, Michael Puckette   +4 more
doaj   +1 more source

Hoverfly (Diptera: Syrphidae) diversity in Tapada da Ajuda, Lisbon - a preliminary study [PDF]

, 2018
Hoverflies are holometabolic insects, whose adults are pollinators feeding on nectar and pollen. Frequently, they are confused with wasps, bees and bumblebees.
Espírito Santo, Gonçalo, Figueiredo, Elisabete, Silva, Elsa Borges da   +2 more
core   +1 more source

Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

BMC Biotechnology, 2022
Background Type I interferons are widely used in research applications and as biotherapeutics. Current assays used to measure interferon concentrations, such as plaque reduction assays and ELISA, are expensive, technically challenging, and may take days ...
Michael Puckette, J. Barrera, M. Schwarz, M. Rasmussen   +3 more
doaj   +1 more source

Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine

Viruses, 2023
African swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source

Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Bio-Protocol, 2021
Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1),
Ayushi Rai , Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel Borca, Douglas Gladue   +8 more
doaj   +1 more source

Folding and insertion thermodynamics of the transmembrane WALP peptide [PDF]

, 2015
The anchor of most integral membrane proteins consists of one or several helices spanning the lipid bilayer. The WALP peptide, GWW(LA)$_n$(L)WWA, is a common model helix to study the fundamentals of protein insertion and folding, as well as helix-helix ...
Chernick M. R., Darnell J. E., Jim Pfaendtner, Markus Deserno, Mikko Karttunen, Tristan Bereau, W. F. Drew Bennett   +6 more
core   +4 more sources

Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain

Viruses, 2019
African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs, African swine fever (ASF). The ASFV Georgia 2007 isolate (ASFV-G) is responsible for the current epidemic situation in Europe and Asia.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Vivian O’Donnell, Lauren G. Holinka, Ediane Silva, Ayushi Rai, Sarah Pruitt, Consuelo Carrillo, Douglas P. Gladue, Manuel V. Borca   +9 more
doaj   +1 more source

Deletion of the ASFV dUTPase Gene E165R from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs

Viruses, 2022
African swine fever (ASF) is a frequently lethal disease of domestic and wild swine currently producing a pandemic affecting pig production in Eurasia.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source