Results 31 to 40 of about 52,107 (249)

Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication

Viruses, 2020
Interactions between the major structural glycoprotein E2 of classical swine fever virus (CSFV) with host proteins have been identified as important factors affecting virus replication and virulence.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Keith Berggren, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source

Effect of Foot-and-Mouth Disease Virus 2B Viroporin on Expression and Extraction of Mammalian Cell Culture Produced Foot-and-Mouth Disease Virus-like Particles

Vaccines, 2022
To improve the production of foot-and-mouth disease (FMD) molecular vaccines, we sought to understand the effects of the FMD virus (FMDV) 2B viroporin in an experimental, plasmid-based, virus-like particle (VLP) vaccine.
Victoria Primavera, Janine Simmons, Benjamin A. Clark, John G. Neilan, Michael Puckette   +4 more
doaj   +1 more source

Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

BMC Biotechnology, 2022
Background Type I interferons are widely used in research applications and as biotherapeutics. Current assays used to measure interferon concentrations, such as plaque reduction assays and ELISA, are expensive, technically challenging, and may take days ...
Michael Puckette, J. Barrera, M. Schwarz, M. Rasmussen   +3 more
doaj   +1 more source

A Highly Effective African Swine Fever Virus Vaccine Elicits a Memory T Cell Response in Vaccinated Swine

Pathogens, 2022
African Swine Fever Virus (ASFV) is the causative agent of a highly contagious and lethal vector-borne disease in suids. Recently, a live attenuated virus strain, developed using the currently circulating, virulent Georgia strain (ASFV-G) with a single ...
Sarah E. Attreed, Christina Silva, Sophia Abbott, Elizabeth Ramirez-Medina, Nallely Espinoza, Manuel V. Borca, Douglas P. Gladue, Fayna Diaz-San Segundo   +7 more
doaj   +1 more source

Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Bio-Protocol, 2021
Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1),
Ayushi Rai , Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel Borca, Douglas Gladue   +8 more
doaj   +1 more source

Deletion of the ASFV dUTPase Gene E165R from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs

Viruses, 2022
African swine fever (ASF) is a frequently lethal disease of domestic and wild swine currently producing a pandemic affecting pig production in Eurasia.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source

Brochure: Plum Run Farms, Inc., "Plum Run Farms," Undated

, 2007
Textual: Brochure, original; 16” x 10.5” (40.6 cm x 26.7 cm)Brochure produced by Plum Run Farms, Inc. entitled "Plum Run Farms" that is undated. This brochure discusses a development outside of Louisville, Kentucky on interstate 64 known as Plum Run ...
Plum Run Farms, Inc.
core   +1 more source

Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine

Viruses, 2023
African swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source

Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain

Viruses, 2019
African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs, African swine fever (ASF). The ASFV Georgia 2007 isolate (ASFV-G) is responsible for the current epidemic situation in Europe and Asia.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Vivian O’Donnell, Lauren G. Holinka, Ediane Silva, Ayushi Rai, Sarah Pruitt, Consuelo Carrillo, Douglas P. Gladue, Manuel V. Borca   +9 more
doaj   +1 more source

Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability

Scientific Reports
For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries ...
Nallely Espinoza, Edward Spinard, Ayushi Rai, Elizabeth Ramirez-Medina, Alyssa Valladares, Amanda Meyers, Manuel V. Borca, Douglas P. Gladue   +7 more
doaj   +1 more source