Results 41 to 50 of about 23,136 (263)

Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB)

Viruses, 2019
Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune response and is critical for virulence in swine.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Lauren G. Holinka, Ryan Baker-Branstetter, Manuel V. Borca, Douglas P. Gladue   +5 more
doaj   +1 more source

Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

BMC Biotechnology, 2022
Background Type I interferons are widely used in research applications and as biotherapeutics. Current assays used to measure interferon concentrations, such as plaque reduction assays and ELISA, are expensive, technically challenging, and may take days ...
Michael Puckette, J. Barrera, M. Schwarz, M. Rasmussen   +3 more
doaj   +1 more source

A Highly Effective African Swine Fever Virus Vaccine Elicits a Memory T Cell Response in Vaccinated Swine

Pathogens, 2022
African Swine Fever Virus (ASFV) is the causative agent of a highly contagious and lethal vector-borne disease in suids. Recently, a live attenuated virus strain, developed using the currently circulating, virulent Georgia strain (ASFV-G) with a single ...
Sarah E. Attreed, Christina Silva, Sophia Abbott, Elizabeth Ramirez-Medina, Nallely Espinoza, Manuel V. Borca, Douglas P. Gladue, Fayna Diaz-San Segundo   +7 more
doaj   +1 more source

Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine

Viruses, 2023
African swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source

Exploring the Molecular Basis of Vesicular Stomatitis Virus Pathogenesis in Swine: Insights from Expression Profiling of Primary Macrophages Infected with M51R Mutant Virus

Pathogens, 2023
Vesicular stomatitis virus (VSV) is an emergent virus affecting livestock in the US. Previously, using a recombinant VSV carrying the M51R mutation in the matrix protein (rNJ0612NME6-M51R), we evaluated the pathogenesis of this virus in pigs. Our results
Lauro Velazquez-Salinas, Gisselle N. Medina, Federico Valdez, Selene Zarate, Shannon Collinson, James J. Zhu, Luis L. Rodriguez   +6 more
doaj   +1 more source

Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Bio-Protocol, 2021
Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1),
Ayushi Rai , Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel Borca, Douglas Gladue   +8 more
doaj   +1 more source

Deletion of the ASFV dUTPase Gene E165R from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs

Viruses, 2022
African swine fever (ASF) is a frequently lethal disease of domestic and wild swine currently producing a pandemic affecting pig production in Eurasia.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source

Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability

Scientific Reports
For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries ...
Nallely Espinoza, Edward Spinard, Ayushi Rai, Elizabeth Ramirez-Medina, Alyssa Valladares, Amanda Meyers, Manuel V. Borca, Douglas P. Gladue   +7 more
doaj   +1 more source

Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain

Viruses, 2019
African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs, African swine fever (ASF). The ASFV Georgia 2007 isolate (ASFV-G) is responsible for the current epidemic situation in Europe and Asia.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Vivian O’Donnell, Lauren G. Holinka, Ediane Silva, Ayushi Rai, Sarah Pruitt, Consuelo Carrillo, Douglas P. Gladue, Manuel V. Borca   +9 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source