Results 271 to 280 of about 173,543 (315)

Induced Pluripotent Stem Cells

2010
Reprogramming of mouse and human somatic cells into induced pluripotent stem (iPS) cells has been possible with retroviral expression of the pluripotency-associated transcription factors Oct4, Sox2, Nanog, and Lin28 as well as Klf4 and c-Myc. iPS cells hold great potential as a model for diseases from the perspective of the individual patient and as an
Zaehres, Holm, Kim, Jeong Beom, Scholer, Hans R.   +2 more
openaire   +3 more sources

Characterization of pluripotent stem cells

Nature Protocols, 2013
Characterization of pluripotent stem cells is required for the registration of stem cell lines and allows for an impartial and objective comparison of the results obtained when generating multiple lines. It is therefore crucial to establish specific, fast and reliable protocols to detect the hallmarks of pluripotency.
Mercè, Martí, Lola, Mulero, Cristina, Pardo, Cristina, Morera, Meritxell, Carrió, Leopoldo, Laricchia-Robbio, Concepcion Rodriguez, Esteban, Juan Carlos, Izpisua Belmonte   +7 more
openaire   +2 more sources

Pluripotent Stem Cell Heterogeneity

2019
Pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, show heterogeneity with respect to their pluripotency, self-renewal ability, and other traits. PSC heterogeneity may exist among cell lines, among cells within a line, and among temporal states of individual cells.
Yohei, Hayashi, Kiyoshi, Ohnuma, Miho K, Furue   +2 more
openaire   +2 more sources

Pluripotent stem cells and their niches

Stem Cell Reviews, 2006
The ability of stem cells to self-renew and to replace mature cells is fundamental to ontogeny and tissue regeneration. Stem cells of the adult organism can be categorized as mono-, bi-, or multipotent, based on the number of mature cell types to which they can give rise.
M William, Lensch, Laurence, Daheron, Thorsten M, Schlaeger   +2 more
openaire   +2 more sources

The development of pluripotent stem cells

Current Opinion in Genetics & Development, 2012
Stem cell biology has many roots, and the current interest in the possible medical and pharmaceutical applications of pluripotent stem cells has far removed origins in the biology of a rare but peculiar type of tumor, the teratomas. The identification of their stem cells and their relationship to the early embryo paved the way, first in the mouse and ...
Paul J, Gokhale, Peter W, Andrews
openaire   +2 more sources

Pluripotent Stem Cells in Mice

2012
Pluripotent stem cells are able to self-renew and to differentiate into all adult cell types. Many studies report data describing these cells and characterize them in molecular terms. Gene expression data of pluripotent and non-pluripotent cells from mouse were assembled.
Rainer, Schmidt, Lena, Scheubert, Mitja, Lustrek, Dirk, Repsilber, Georg, Fuellen   +4 more
openaire   +2 more sources

Cryobanking Pluripotent Stem Cells

2017
Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has
Crook, Jeremy Micah, Tomaskovic-Crook, Eva, Ludwig, Tenneille   +2 more
openaire   +3 more sources

Pluripotent Stem Cells for Cell Therapy

2021
In an increasingly geriatric population, in which elderly people frequently face chronic diseases and degenerative conditions, cell therapies as part of novel regenerative medicine approaches are of great interest. Even though today's cell therapies mostly rely on adult stem cells like the mesenchymal stem cells or primary somatic cells, pluripotent ...
openaire   +2 more sources

Origins of pluripotent stem cells.

Minerva ginecologica, 2011
Different types of pluripotent stem cells can be identified and cultured in vitro. Here an overview is presented of the various pluripotent stem cells types. Embryonal carcinoma (EC) cells that have been cultured in vitro provided the groundwork for future pluripotent cell cultures.
Roelen, B.A.J., Chuva de Sousa Lopes, S.M.   +1 more
openaire   +2 more sources

Generation of Induced Pluripotent Stem Cells

2014
Induced pluripotent stem cells (iPSCs) are generated from somatic cells that have been reprogrammed by the ectopic expression of defined embryonic transcription factors. This technology has provided investigators with a powerful tool for modeling disease and developing treatments for human disorders.
openaire   +3 more sources