Results 71 to 80 of about 980,977 (401)

A novel -mutated human induced pluripotent stem cell model for understanding -mutated tumors

Tumor Biology, 2020
A missense mutation of the guanine nucleotide binding protein alpha stimulating activity polypeptide 1 ( GNAS ) gene, typically Arg201Cys or Arg201His (R201H/R201C), leads to constitutive activation of the Gsα-cyclic AMP (cAMP) signaling pathway that ...
Katsuhito Watanabe, Takashi Nakamura, Shoko Onodera, Akiko Saito, Takahiko Shibahara, Toshifumi Azuma   +5 more
doaj   +1 more source

Our Bodies, Our Cells: FDA Regulation of Autologous Adult Stem Cell Therapies [PDF]

, 2013
Stem cells have been an endless source of fascination and controversy since Dolly the sheep was cloned in 1996. This month’s announcement of a cloned human embryo from a single skin cell came on the heels of Sir John B. Gurdon and Dr.
Chirba, Mary Ann, Noble, Alice
core  

Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors. [PDF]

, 2012
The pluripotent nature of human embryonic stem cells (hESCs) makes them convenient for deriving therapeutically relevant cells. Here we show using Wnt reporter hESC lines that the cells are heterogeneous with respect to endogenous Wnt signalling activity.
Ardehali, Reza, Blauwkamp, Timothy A, Nigam, Shelly, Nusse, Roel, Weissman, Irving L   +4 more
core   +1 more source

miRNA in Pluripotent Stem Cells [PDF]

Regenerative Medicine, 2010
Embryonic stem cells and induced pluripotent stem cells are characterized by their ability to self-renew and differentiate into any cell type. The molecular mechanism behind this process is a complex interplay between the transcriptional factors with epigenetic regulators and signaling pathways.
Uma, Lakshmipathy, Jonathan, Davila, Ronald P, Hart   +2 more
openaire   +2 more sources

Methylation biomarkers can distinguish pleural mesothelioma from healthy pleura and other pleural pathologies

Molecular Oncology, EarlyView.
We developed and validated a DNA methylation–based biomarker panel to distinguish pleural mesothelioma from other pleural conditions. Using the IMPRESS technology, we translated this panel into a clinically applicable assay. The resulting two classifier models demonstrated excellent performance, achieving high AUC values and strong diagnostic accuracy.
Janah Vandenhoeck, Nele De Meulenaere, Thomas Vanpoucke, Joe Ibrahim, Dieter Peeters, Suresh Krishan Yogeswaran, Wen Wen, Paul Van Schil, Jeroen M. H. Hendriks, Jo Raskin, Jan van Meerbeeck, Guy Van Camp, Ken Op de Beeck   +12 more
wiley   +1 more source

Human induced pluripotent stem cell-derived atrial cardiomyocytes carrying an SCN5A mutation identify nitric oxide signaling as a mediator of atrial fibrillation [PDF]

, 2021
Liang Hong, Meihong Zhang, Olivia T Ly, Hanna Chen, Arvind Sridhar, Erin Lambers, Brandon Chalazan, Seock‐Won Youn, Mark Maienschein‐Cline, Leonid Feferman, Sang‐Ging Ong, Joseph C. Wu, Jalees Rehman, Dawood Darbar   +13 more
openalex   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

3D bioprinted human cortical neural constructs derived from induced pluripotent stem cells [PDF]

, 2019
Bioprinting techniques use bioinks made of biocompatible non-living materials and cells to build 3D constructs in a controlled manner and with micrometric resolution.
Benedetti, Maria Cristina, Brighi, Carlo, Colosi, Cristina, Di Angelantonio, Silvia, Ghirga, Silvia, Rosa, Alessandro, Rosito, Maria, Salaris, Federico, Soloperto, Alessandro, Turris, Valeria de   +9 more
core   +1 more source

Generation of a human embryonic stem cell line (WAe009-A-3B) carrying homozygous TNNT2 gene knockout by CRISPR/Cas9 editing

Stem Cell Research
The TNNT2 gene encodes cardiac troponin T (cTnT), a critical protein in cardiac muscle contraction. Mutations in TNNT2 are associated with various cardiomyopathies, including hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), which ...
Didaer Talanaite, Hongyue Wang, Man Qi, Feng Lan   +3 more
doaj   +1 more source

Induced pluripotent stem (iPS) cells from human fetal stem cells [PDF]

, 2016
Pluripotency defines the ability of stem cells to differentiate into all the lineages of the three germ layers and self-renew indefinitely. Somatic cells can regain the developmental potential of embryonic stem cells following ectopic expression of a set
Guillot, PV
core   +1 more source