Results 111 to 120 of about 4,071 (219)

The protein‐only RNase Ps, endonucleases that cleave pre‐tRNA: Biological relevance, molecular architectures, substrate recognition and specificity, and protein interactomes

open access: yesWIREs RNA, Volume 15, Issue 2, March/April 2024.
Three distinct classes of PRORP evolved to cleave the 5′ leader sequence from pre‐tRNA. Beyond available structure, kinetic, and thermodynamic data, there is more to learn about the protein‐interaction network, substrate specificity, and disease‐linked mutations related to PRORP.
Catherine A. Wilhelm   +4 more
wiley   +1 more source

Genome-Wide Study of mRNA Degradation and Transcript Elongation in Escherichia coli [PDF]

open access: yes, 2015
An essential part of gene expression is the coordination of RNA synthesis and degradation, which occurs in the same cellular compartment in bacteria.
Chen, Huiyi   +3 more
core   +2 more sources

Erythrocyte metabolism

open access: yesActa Physiologica, Volume 240, Issue 3, March 2024.
Abstract Our aim is to present an updated overview of the erythrocyte metabolism highlighting its richness and complexity. We have manually collected and connected the available biochemical pathways and integrated them into a functional metabolic map.
Panagiotis N. Chatzinikolaou   +7 more
wiley   +1 more source

Landscape of RNA polyadenylation in E-coli [PDF]

open access: yes, 2016
Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated.
Aurell, Erik   +5 more
core   +2 more sources

Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes

open access: yesBioTechniques, 2012
A coupled enzyme assay able to monitor the kinetics of reactions catalyzed by phosphate- or pyrophosphate-releasing enzymes is presented here.
Antonio S. Guillén Suárez   +4 more
doaj   +1 more source

Escherichia coli strains with precise domain deletions in the ribonuclease RNase E can achieve greatly enhanced levels of membrane protein production

open access: yesProtein Science, Volume 33, Issue 2, February 2024.
Abstract Escherichia coli is one of the most widely utilized hosts for production of recombinant membrane proteins (MPs). Bacterial MP production, however, is usually accompanied by severe toxicity and low‐level volumetric accumulation. In previous work, we had discovered that co‐expression of RraA, an inhibitor of the RNA‐degrading activity of RNase E,
Eleni Vasilopoulou   +2 more
wiley   +1 more source

A comprehensive study of the interactions in the B. subtilis degradosome with special emphasis on the role of the small proteins SR1P and SR7P

open access: yesMolecular Microbiology, Volume 121, Issue 1, Page 40-52, January 2024.
The RNA degradosome of B. subtilis comprises the major endoribonuclease RNase Y, RNases J1 and J2, PNPase, CshA helicase, and the glycolytic enzymes enolase and phosphofructokinase. Additionally, glyceraldehyde 3‐phosphate dehydrogenase A (GapA) is integrated under specific conditions.
Inam Ul Haq   +2 more
wiley   +1 more source

Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria

open access: yesNature Communications, 2018
G-rich RNAs encoded in mitochondrial DNA are prone to form four-stranded structures called G-quadruplexes (G4s). Here the authors show using in vitro and in vivo approaches that GRSF1 promotes melting of G4 RNA structures in mtRNAs, thus leading to their
Zbigniew Pietras   +8 more
doaj   +1 more source

Insights into Xanthomonas axonopodis pv. citri biofilm through proteomics [PDF]

open access: yes, 2013
Background: Xanthomonas axonopodis pv. citri (X. a. pv. citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and
Garavaglia, Betiana Soledad   +6 more
core   +1 more source

Oxygen consumption and usage during physical exercise: the balance between oxidative stress and ROS-dependent adaptive signaling. [PDF]

open access: yes, 2013
The complexity of human DNA has been affected by aerobic metabolism, including endurance exercise and oxygen toxicity. Aerobic endurance exercise could play an important role in the evolution of Homo sapiens, and oxygen was not important just for ...
Atalay, Mustafa   +4 more
core   +1 more source

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