Polynucleotide Phosphorylase from a Cyanobacterium (Synechococcus sp.): Subunit Composition and Properties [PDF]
, 1982 Polynucleotide phosphorylase from cells of the cyanobacterium Synechococcus sp. has been purified 1400-fold by an improved procedure. The enzyme purified to homogeneity and lacking nuclease, phosphatase and protease contaminations reveals a single band ...
Nolden, W.-T., Richter, G.
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Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
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Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria. [PDF]
, 2017 SCL25A46 is a mitochondrial carrier protein that surprisingly localizes to the outer membrane and is distantly related to Ugo1. Here we show that a subset of SLC25A46 interacts with mitochondrial dynamics components and the MICOS complex.
Claypool, Steven M +6 more
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mBio, 2021 Here, we show that RNase E, RhlB, and PNPase act together as components of the multienzyme RNA degradosome in polyribosome-dependent clustering to form puncta on the inner cytoplasmic membrane.
Lina Hamouche +2 more
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Construction of microbial platform for an energy-requiring bioprocess: practical 2′-deoxyribonucleoside production involving a C−C coupling reaction with high energy substrates [PDF]
, 2012 BACKGROUND: Reproduction and sustainability are important for future society, and bioprocesses are one technology that can be used to realize these concepts.
Jun Ogawa +8 more
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Cell Reports, 2015 Oocyte factors not only drive somatic cell nuclear transfer reprogramming but also augment the efficiency and quality of induced pluripotent stem cell (iPSC) reprogramming.
Swea-Ling Khaw +4 more
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Computational evolution of an RNA-binding protein towards enhanced oxidized-RNA binding
Computational and Structural Biotechnology Journal, 2020 The oxidation of RNA has been implicated in the development of many diseases. Among the four ribonucleotides, guanosine is the most susceptible to oxidation, resulting in the formation of 8-oxo-7,8-dihydroguanosine (8-oxoG). Despite the limited knowledge
Juan C. Gonzalez-Rivera +9 more
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Frontiers in Microbiology, 2016 Post-transcriptional regulation enables bacteria to quickly response to environmental stresses. Polynucleotide phosphorylase (PNPase), which contains an N-terminal catalytic core and C-terminal RNA binding KH-S1 domains, is involved in RNA processing ...
Ronghao eChen +10 more
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Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis [PDF]
, 2018 Endonucleolytic cleavage within polycistronic mRNAs can lead to differential stability, and thus discordant abundance, among cotranscribed genes. RNase Y, the major endonuclease for mRNA decay in Bacillus subtilis, was originally identified for its ...
DeLoughery, Aaron +3 more
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Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 2018 Background 8‐Aminoguanosine and 8‐aminoguanine are K+‐sparing natriuretics that increase glucose excretion. Most effects of 8‐aminoguanosine are due to its metabolism to 8‐aminoguanine.
Edwin K. Jackson +3 more
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