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Electron Microscopy Reviews, 1991
In the last few years the role of pollen and the pollen tube in the fertilization process in higher plants has received considerable attention. By ultrastructural, biochemical and immunofluorescent investigations it has been shown that a cytoskeletal apparatus plays a central role in pollen tube growth.
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In the last few years the role of pollen and the pollen tube in the fertilization process in higher plants has received considerable attention. By ultrastructural, biochemical and immunofluorescent investigations it has been shown that a cytoskeletal apparatus plays a central role in pollen tube growth.
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Actin in Pollen and Pollen Tubes
2000Flowering plants rely on the highly polarized process of pollen tube growth for delivery of the sperm cells and thus for sexual reproduction. Although it has long been known that the actin cytoskeleton is necessary for pollen grain germination and tube growth, the precise structure of the actin arrays and their regulation by actin-associated proteins ...
Luis Vidali, Peter K. Hepler
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Culture of Pollen Tubes for Chromosomal Analysis at the Pollen Tube Division
Stain Technology, 1953It is possible to grow pollen tubes routinely for cytological analysis of nuclei at the pollen tube division. Pollen has been grown successfully after temperature, pressure, gas, moisture, and radiation treatments. The technic for growing Tradescantia pollen is described, but any method is satisfactory which ensures that: (a) the pollen is kept dry ...
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Pollen tubes and the physical world
Trends in Plant Science, 2011The primary goal of our previous opinion paper (Winship, L.J. et al. (2010) Trends Plant Sci. 15, 363-369) [1] was to put two models for the control of pollen tube growth on the same theoretical and biophysical footing, and to then test both for consistency with basic principles and with experimental data.
Lawrence J, Winship +3 more
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Calcium entry into pollen tubes
Trends in Plant Science, 2012Growing pollen tubes require calcium to maintain a tip-focused cytosolic gradient and as a constituent of the constantly expanding cell wall. Advances in cell and molecular biology as well as electrophysiology implicate several candidate channels and receptors in the flow of calcium into the cell.
Peter K, Hepler +3 more
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POLLEN GERMINATION AND TUBE GROWTH
Annual Review of Plant Physiology and Plant Molecular Biology, 1997▪ Abstract Many aspects of Angiosperm pollen germination and tube growth are discussed including mechanisms of dehydration and rehydration, in vitro germination, pollen coat compounds, the dynamic involvement of cytoskeletal elements (actin, microtubules), calcium ion fluxes, extracellular matrix elements (stylar arabinogalactan proteins), and control
Loverine P., Taylor, Peter K., Hepler
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Protoplasma, 2001
Actin microfilaments (MFs) are essential for the growth of the pollen tube. Although it is well known that MFs, together with myosin, deliver the vesicles required for cell elongation, it is becoming evident that the polymerization of new actin MFs, in a process that is independent of actomyosin-dependent vesicle translocation, is also necessary for ...
L, Vidali, P K, Hepler
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Actin microfilaments (MFs) are essential for the growth of the pollen tube. Although it is well known that MFs, together with myosin, deliver the vesicles required for cell elongation, it is becoming evident that the polymerization of new actin MFs, in a process that is independent of actomyosin-dependent vesicle translocation, is also necessary for ...
L, Vidali, P K, Hepler
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The pollen tube paradigm revisited
Current Opinion in Plant Biology, 2012The polar growth process characterizing pollen tube elongation has attracted numerous modeling attempts over the past years. While initial models focused on recreating the correct cellular geometry, recent models are increasingly based on experimentally assessed cellular parameters such as the dynamics of signaling processes and the mechanical ...
Jens, Kroeger, Anja, Geitmann
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Fluorescence Microscopy in the Study of Pollen Grains and Pollen Tubes
Stain Technology, 1962Fresh pollen gains were either crushed directly in a 0.01% solution of acridine orange (0.1 M phosphate-citrate buffer, pH 5.2-5.4) or they were germinated previously in 5-25% sucrose solution (glass-distilled water of pH 5.0-6.0 with 100 ppm H3BO3) inside moist incubating chambers at 24-30° C. Observations and records were made by using ultraviolet or
P N, BHADURI, P K, BHANJA
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Pollen Germination and Pollen Tube Growth
2001Pollen, the male gametophyte of higher plants, is a biological system playing a central role in sexual plant reproduction (Cresti et al., 1992). The understanding of the mechanisms controlling the various aspects of pollen tube development has a direct relevance to biotechnological applications since it represents a starting point to modify crop ...
A. Moscatelli, M. Cresti
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