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Fluorescence Microscopy in the Study of Pollen Grains and Pollen Tubes
Stain Technology, 1962Fresh pollen gains were either crushed directly in a 0.01% solution of acridine orange (0.1 M phosphate-citrate buffer, pH 5.2-5.4) or they were germinated previously in 5-25% sucrose solution (glass-distilled water of pH 5.0-6.0 with 100 ppm H3BO3) inside moist incubating chambers at 24-30° C. Observations and records were made by using ultraviolet or
P N, BHADURI, P K, BHANJA
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Pollen Germination and Pollen Tube Growth
2001Pollen, the male gametophyte of higher plants, is a biological system playing a central role in sexual plant reproduction (Cresti et al., 1992). The understanding of the mechanisms controlling the various aspects of pollen tube development has a direct relevance to biotechnological applications since it represents a starting point to modify crop ...
A. Moscatelli, M. Cresti
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The Cytoskeleton of Pollen Grains and Pollen Tubes
1992The cytoskeleton is assumed to be involved in many internal functions of the eukaryotic cell, e.g. organelle movement, mitotic and meiotic division, cell morphogenesis and cell growth (Dustin, 1984; Bershadsky and Vasiliev, 1988). Examination of a broad variaty of mono- and dicotyledones has revealed the presence of microtubules and/or actin filaments ...
E. S. Pierson, Y. Q. Li
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Integration of ovular signals and exocytosis of a Ca2+ channel by MLOs in pollen tube guidance
Nature Plants, 2020Jiang-Guo Meng +5 more
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Pollen Tube Growth in vivo as a Measure of Pollen Viability
Science, 1962Observation by fluorescence of tomato pollen tubes in fixed styles clearly showed that all pollen samples grew regardless of age and fruit or seed setting potential. Methods commonly used to evaluate pollen viability, such as staining and germination, may predict growth in the style but do not always predict seed setting ability.
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Pollen Germination and Pollen-Tube Growth
1987Publisher Summary Pollen germination and pollen-tube growth events when viewed either in culture or in the style are considered as biochemically, physiologically, and structurally complex. The various processes involved are each independent in some measure, but still all are interactive—namely, the successful attainment of the delivery of two male ...
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1973
The pollen tube of flowering plants may grow through as much as 50 cm of the stylar tissue, enter the micropyle of an ovule and then discharge from its tip two non-motile sperm cells in the immediate vicinity of an unfertilized egg. This review is concerned with the factors that direct the pollen tube to the egg.
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The pollen tube of flowering plants may grow through as much as 50 cm of the stylar tissue, enter the micropyle of an ovule and then discharge from its tip two non-motile sperm cells in the immediate vicinity of an unfertilized egg. This review is concerned with the factors that direct the pollen tube to the egg.
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CORNICHON sorting and regulation of GLR channels underlie pollen tube Ca2+ homeostasis
Science, 2018Michael M. Wudick +11 more
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Multilayered signaling pathways for pollen tube growth and guidance
Plant Reproduction, 2018Hong-Ju Li, Jiang-Guo Meng, Wei-cai Yang
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