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The Use of Biotinylated Poly(ADP-Ribose) for Studies on Poly(ADP-Ribose)-Protein Interaction
Analytical Biochemistry, 1994Poly(ADP-ribose) is routinely detected by the use of radioactive polymers formed from labeled substrates. In this report a simple and time-saving method for the biotinylation and the detection of poly(ADP-ribose) on blots is described. The polymer modified by light-induced reaction with photobiotin was colorimetrically detected and quantified, using ...
F M, Narendja, G, Sauermann
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Trapping Poly(ADP-Ribose) Polymerase
The Journal of Pharmacology and Experimental Therapeutics, 2015Recent findings indicate that a major mechanism by which poly(ADP-ribose) polymerase (PARP) inhibitors kill cancer cells is by trapping PARP1 and PARP2 to the sites of DNA damage. The PARP enzyme-inhibitor complex "locks" onto damaged DNA and prevents DNA repair, replication, and transcription, leading to cell death.
Yuqiao, Shen +2 more
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The Life History of Poly(ADP-ribose)
2020We present arguments and data in support of the following sequence of events in ghost cells. Poly(ADP-ribose, ADPR) synthetase, activated by binding to DNA at a break or other anomaly, synthesizes chains of polymer upon itself, adding new residues at the proximal 1" terminus of the growing chain.
M, Ikejima +4 more
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Poly(ADP-Ribose) Polymerase Inhibitors
Current Medicinal Chemistry, 2003Poly(ADP-ribose) polymerase-1 (PARP-1) is the principal member of the PARP enzyme family consisting of PARP-1 and several recently identified novel poly(ADP-ribosyl)ating enzymes. PARP-1 functions as a DNA damage sensor and signalling molecule. Upon binding to DNA breaks, activated PARP cleaves NAD(+) into nicotinamide and ADP-ribose and polymerizes ...
Garry J, Southan, Csaba, Szabó
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The Histochemical Journal, 1996
Poly(ADP-ribose) polymerase catalyses the formation of ADP-ribose polymers covalently attached to various nuclear proteins, using NAD+ as substrate. The activity of this enzyme is strongly stimulated upon binding to DNA single or double strand breaks. Poly(ADP-ribosyl)ation is an immediate cellular response to DNA damage and is thought to be involved ...
J H, Küpper +3 more
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Poly(ADP-ribose) polymerase catalyses the formation of ADP-ribose polymers covalently attached to various nuclear proteins, using NAD+ as substrate. The activity of this enzyme is strongly stimulated upon binding to DNA single or double strand breaks. Poly(ADP-ribosyl)ation is an immediate cellular response to DNA damage and is thought to be involved ...
J H, Küpper +3 more
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New Insights into the Roles of NAD+-Poly(ADP-ribose) Metabolism and Poly(ADP-ribose) Glycohydrolase
Current Protein & Peptide Science, 2016Accumulating evidence has suggested the fundamental functions of NAD+-poly(ADP-ribose) metabolism in cellular and physiological processes, including energy homeostasis, signal transduction, DNA transaction, genomic stability and cell death or survival. The NAD+ biosynthesis and poly(ADP-ribose) [(ADP-R)n] turnover are tightly controlled by several key ...
Seiichi, Tanuma +5 more
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Monoclonal Antibodies against Poly(ADP-ribose) Recognize Different Structures of Poly(ADP-ribose)
2020The characteristics of two monoclonal antibodies to poly(ADP-Rib) and the various structures of poly(ADP-Rib) recognized by these monoclonal antibodies have been examined. One antibody, IgG monoclonal antibody 10H, reacted with most parts of poly (ADP-Rib) molecules, and its binding was only slightly inhibited by Ado(P)-Rib-P, a monomer unit of the ...
H, Kawamitsu +3 more
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Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions
1996Methods are described for the preparation of poly(ADP-ribose) and for the preparation of biotinylated poly(ADP-ribose). Poly(ADP-ribose) is covalently modified to its biotinylated derivative by light-induced reaction of photobiotin with the polymer.
Frank M. Narendja, Georg Sauermann
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Purification of Recombinant Poly(ADP-Ribose) Polymerases
2011The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude extract are separated on a Heparine Sepharose™ column. The PARP-containing fractions are then affinity purified on a 3-
Amé, Jean-Christophe +3 more
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Enzymatic properties of poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase on chromatin
1992The catabolism of NAD+ in cells is carried out by poly(ADP-ribose) polymerase (PARP) which converts NAD+ into poly(ADP-ribose) in response to DNA damage. All the evidence that has accumulated up to now indicates that the degradation of poly(ADP-ribose) in vivo is carried out by poly(ADP-ribose) glycohydrolase (the glycohydrolase) and not by a ...
Hélène Thomassin +6 more
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