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Monoclonal Antibodies against Poly(ADP-ribose) Recognize Different Structures of Poly(ADP-ribose)
2020The characteristics of two monoclonal antibodies to poly(ADP-Rib) and the various structures of poly(ADP-Rib) recognized by these monoclonal antibodies have been examined. One antibody, IgG monoclonal antibody 10H, reacted with most parts of poly (ADP-Rib) molecules, and its binding was only slightly inhibited by Ado(P)-Rib-P, a monomer unit of the ...
H, Kawamitsu +3 more
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Poly(ADP-ribose) signaling in cell death
Molecular Aspects of Medicine, 2013Poly(ADP-ribosyl)ation (PARylation) is a reversible protein modification carried out by the concerted actions of poly(ADP-ribose) polymerase (PARP) enzymes and poly(ADP-ribose) (PAR) decomposing enzymes such as PAR glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3).
Virág, László +3 more
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Enzymatic properties of poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase on chromatin
1992The catabolism of NAD+ in cells is carried out by poly(ADP-ribose) polymerase (PARP) which converts NAD+ into poly(ADP-ribose) in response to DNA damage. All the evidence that has accumulated up to now indicates that the degradation of poly(ADP-ribose) in vivo is carried out by poly(ADP-ribose) glycohydrolase (the glycohydrolase) and not by a ...
Hélène Thomassin +6 more
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Catching mono- and poly-ADP-ribose readers with synthetic ADP-ribose baits
Molecular Cell, 2021ADP-ribosylation is an essential post-translational modification that comes in two varieties: mono-ADP-ribosylation (MAR) and poly-ADP-ribosylation (PAR). Modular interaction domains that read MAR and PAR modifications are critical for interpreting the language of ADP-ribosylation. Kliza et al.
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Purification of Recombinant Poly(ADP-Ribose) Polymerases
2011The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude extract are separated on a Heparine Sepharose™ column. The PARP-containing fractions are then affinity purified on a 3-
Amé, Jean-Christophe +3 more
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Noncovalent Protein Interaction with Poly(ADP-Ribose)
2011Compared to most common posttranslational modifications of proteins, a peculiarity of poly(ADP-ribosyl)ation is the molecular heterogeneity and complexity of the reaction product, poly(ADP-ribose) (PAR). In fact, protein-bound PAR consists of variously sized (2-200 ADP-ribose residues) linear or branched molecules, negatively charged at physiological ...
Althaus, F R, Malanga, M
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1987
In eukaryotic cells, two different types of enzymes are known to attack poly-(ADP-ribose). The first enzyme, poly(ADP-ribose) glycohydrolase, cleaves ri-bose-ribose bonds of both linear and branched portions of poly(ADP-ribose) polymers by an exoglycosidic hydrolysis mode.
Felix R. Althaus, Christoph Richter
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In eukaryotic cells, two different types of enzymes are known to attack poly-(ADP-ribose). The first enzyme, poly(ADP-ribose) glycohydrolase, cleaves ri-bose-ribose bonds of both linear and branched portions of poly(ADP-ribose) polymers by an exoglycosidic hydrolysis mode.
Felix R. Althaus, Christoph Richter
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Regulation of poly(ADP-ribose) metabolism by poly(ADP-ribose) glycohydrolase: where and when?
Cellular and molecular life sciences : CMLS, 2005Poly(ADP-ribose) glycohydrolase (PARG) is a catabolic enzyme that cleaves ADP-ribose polymers formed by members of the PARP family of enzymes. Despite its discovery and subsequent partial purification in the 1970s and the cloning of its single gene in the late 1990s, little is known about the role of PARG in cell function.
M-E, Bonicalzi +3 more
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1985
Nucleoids are nuclei-like structures produced from cells lysed with nonionic detergents in the presence of high salt concentrations. By this treatment, histones and most of the nonhistone proteins are removed [3]. They contain naked, histone-free DNA, RNA, and a few proteins [1]. DNA is supercoiled and attached to a cage of residual proteins and RNA [5]
Gordana Brkić +2 more
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Nucleoids are nuclei-like structures produced from cells lysed with nonionic detergents in the presence of high salt concentrations. By this treatment, histones and most of the nonhistone proteins are removed [3]. They contain naked, histone-free DNA, RNA, and a few proteins [1]. DNA is supercoiled and attached to a cage of residual proteins and RNA [5]
Gordana Brkić +2 more
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Poly(ADP-ribose): A Dynamic Trigger for Biomolecular Condensate Formation
Trends in Cell Biology, 2020Anthony K L Leung
exaly