Results 101 to 110 of about 722,396 (378)

A rapid 3% polyacrylamide slab gel electrophoresis method for high through put screening of LDL phenotype

open access: yesLipids in Health and Disease, 2008
Background Small dense LDL is reported to be associated with increased coronary artery disease risk by various epidemiological studies. The gold standard for separation and identification of LDL subtypes in plasma is ultracentrifugation which is a ...
Gupta Ruby   +3 more
doaj   +1 more source

Phosphoproteins and protein-kinase activity in isolated envelopes of pea (Pisum sativum L.) chloroplasts [PDF]

open access: yes, 1985
A protein kinase was found in envelope membranes of purified pea (Pisum sativum L.) chloroplasts. Separation of the two envelope membranes showed that most of the enzyme activity was localized in the outer envelope.
D.G. Robinson   +17 more
core   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

open access: yesFEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama   +7 more
wiley   +1 more source

Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE

open access: yesBio-Protocol, 2018
The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system.
Marjaana Rantala   +2 more
doaj   +1 more source

Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography [PDF]

open access: yes, 2005
Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC).
Abdullah, Norhani   +4 more
core   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Importance of geometry of the extracellular matrix in endochondral bone differentiation. [PDF]

open access: yes, 1984
Subcutaneous implantation of coarse powders (74-420 micron) of demineralized diaphyseal bone matrix resulted in the local differentiation of endochondral bone.
Reddi, AH, Sampath, TK
core   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

CHARACTERISATION OF IBD VIRAL PROTEINS IN SDS POLYACRYLAMIDE GEL ELECTROPHORESIS [PDF]

open access: yesJournal of Veterinary and Animal Sciences, 2004
The field isolates of IBD virus was characterised using protein profile study. Four structural polypeptides of IBD virus with molecular weights of 91 K (VP1), 39 K (VP,), 32 K (VP3), and 28 K (VP4) and the additional polypeptide 47 K (VPX) preceusor ...
M. Parthiban, V. Thiagarajan
doaj  

RRP9 Promotes Esophageal Squamous Cell Carcinoma Progression through E2F1 Transcriptional Regulation of CDK1

open access: yesAdvanced Biology, EarlyView.
The study reveals that RRP9 is abnormally highly expressed in ESCC tissues and is closely associated with poor prognosis in patients. Furthermore, it is found that RRP9 promotes ESCC progression through enhancing the E2F1‐mediated transcriptional regulation of CDK1.
Gang He   +14 more
wiley   +1 more source

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