Results 11 to 20 of about 65,820 (278)
Deciphering the impact of genetic variation on human polyadenylation using APARENT2
Genome Biology, 2022 Background 3′-end processing by cleavage and polyadenylation is an important and finely tuned regulatory process during mRNA maturation. Numerous genetic variants are known to cause or contribute to human disorders by disrupting the cis-regulatory code ...
Johannes Linder +3 more
doaj +2 more sources
Benchmarking sequencing methods and tools that facilitate the study of alternative polyadenylation
Genome Biology, 2021 Background Alternative cleavage and polyadenylation (APA), an RNA processing event, occurs in over 70% of human protein-coding genes. APA results in mRNA transcripts with distinct 3′ ends.
Ankeeta Shah +3 more
doaj +2 more sources
Template-switching artifacts resemble alternative polyadenylation
BMC Genomics, 2019 Background Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally ...
Zsolt Balázs +5 more
doaj +2 more sources
Polyadenylation and nuclear export of mRNAs [PDF]
Journal of Biological Chemistry, 2019 In eukaryotes, the separation of translation from transcription by the nuclear envelope enables mRNA modifications such as capping, splicing, and polyadenylation. These modifications are mediated by a spectrum of ribonuclear proteins that associate with preRNA transcripts, coordinating the different steps and coupling them to nuclear export, ensuring ...
M. Stewart
openaire +4 more sources
Mechanism of STMN2 cryptic splice-polyadenylation and its correction for TDP-43 proteinopathies
Science, 2023 Loss of nuclear TDP-43 is a hallmark of neurodegeneration in TDP-43 proteinopathies, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).
Michael W. Baughn +23 more
semanticscholar +1 more source
Context-specific regulation and function of mRNA alternative polyadenylation
Nature reviews. Molecular cell biology, 2022 Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3′ untranslated regions (UTRs). The expression of alternative 3′ UTR isoforms is highly cell type specific and is further controlled in a ...
Sibylle Mitschka, C. Mayr
semanticscholar +1 more source
Spatial mapping of the total transcriptome by in situ polyadenylation
Nature Biotechnology, 2022 Spatial RNA sequencing is extended beyond poly-A transcripts to capture the full transcriptome. Spatial transcriptomics reveals the spatial context of gene expression, but current methods are limited to assaying polyadenylated (A-tailed) RNA transcripts.
David W. McKellar +6 more
semanticscholar +1 more source
Nature Genetics, 2021 Genome-wide association studies have identified thousands of noncoding variants associated with human traits and diseases. However, the functional interpretation of these variants is a major challenge. Here, we constructed a multi-tissue atlas of human 3′
Lei Li +12 more
semanticscholar +1 more source
Alternative polyadenylation: methods, mechanism, function, and role in cancer
Journal of experimental & clinical cancer research : CR, 2021 Occurring in over 60% of human genes, alternative polyadenylation (APA) results in numerous transcripts with differing 3’ends, thus greatly expanding the diversity of mRNAs and of proteins derived from a single gene.
Yi Zhang +6 more
semanticscholar +1 more source
The landscape of alternative polyadenylation in single cells of the developing mouse embryo
Nature Communications, 2021 3′ untranslated regions (3′ UTRs) post-transcriptionally regulate mRNA stability, localization, and translation rate. While 3′-UTR isoforms have been globally quantified in limited cell types using bulk measurements, their differential usage among cell ...
Vikram Agarwal +3 more
semanticscholar +1 more source