Results 21 to 30 of about 62,357 (236)

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

eLife, 2020
Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Matthew T Parker, Katarzyna Knop, Anna V Sherwood, Nicholas J Schurch, Katarzyna Mackinnon, Peter D Gould, Anthony JW Hall, Geoffrey J Barton, Gordon G Simpson   +8 more
doaj   +1 more source

Long-Read RNA Sequencing Identifies Polyadenylation Elongation and Differential Transcript Usage of Host Transcripts During SARS-CoV-2 In Vitro Infection

Frontiers in Immunology, 2022
Better methods to interrogate host-pathogen interactions during Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infections are imperative to help understand and prevent this disease.
Jessie J.-Y. Chang, Josie Gleeson, Daniel Rawlinson, Ricardo De Paoli-Iseppi, Chenxi Zhou, Francesca L. Mordant, Sarah L. Londrigan, Michael B. Clark, Kanta Subbarao, Kanta Subbarao, Timothy P. Stinear, Lachlan J. M. Coin, Lachlan J. M. Coin, Lachlan J. M. Coin, Lachlan J. M. Coin, Miranda E. Pitt   +15 more
doaj   +1 more source

Positive Feedback Stimulation of Ccnb1 and Mos mRNA Translation by MAPK Cascade During Mouse Oocyte Maturation

Frontiers in Cell and Developmental Biology, 2020
In mammalian species, both the maturation promoting factor (MPF) and the mitogen-activated protein kinase (MAPK) cascade play critical roles in modulating oocyte meiotic cell-cycle progression. MPF is a critical heterodimer composed of CDK1 and cyclin B1.
Lan-Rui Cao, Jun-Chao Jiang, Heng-Yu Fan
doaj   +1 more source

Template-switching artifacts resemble alternative polyadenylation

BMC Genomics, 2019
Background Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally ...
Zsolt Balázs, Dóra Tombácz, Zsolt Csabai, Norbert Moldován, Michael Snyder, Zsolt Boldogkői   +5 more
doaj   +1 more source

The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation [PDF]

, 2003
Cleavage and polyadenylation factor (CPF) is a multi-protein complex that functions in pre-mRNA 3'-end formation and in the RNA polymerase II (RNAP II) transcription cycle.
Sadowski, Martin, Kyburz, A., Kyburz, Andrea, Dichtl, B., Keller, W., Keller, Walter, Sadowski, M., Dichtl, Bernhard   +7 more
core   +1 more source

Variable 3’polyadenylation of Wheat yellow mosaic virus and its novel effects on translation and replication

Virology Journal, 2019
Background Polyadenylation influences many aspects of mRNA as well as viral RNA. variable polyadenylation at the 3' end have been reported in RNA viruses.
Guowei Geng, Chengming Yu, Xiangdong Li, Xuefeng Yuan   +3 more
doaj   +1 more source

Differential polyadenylation of ribosomal RNA during post-transcriptional processing in Leishmania. [PDF]

, 2005
The protozoan parasite Leishmania belongs to the most ancient eukaryotic lineages and this is reflected in several distinctive biological features, such as eukaryotic polycistronic transcription and RNA trans-splicing.
Laurent, T., Chappuis, F., Llanos-Cuentas, A., Llanos-Cuentas, A, V. YARDLEY, F. CHAPPUIS, Laurent, T, T. LAURENT, Dujardin, J. C., Vandesompele, J, J.-C. DUJARDIN, DE DONCKER, S, S. RIJAL, Decuypere, S., S. DECUYPERE, Yardley, V, De Donckeri, S, Vandesompele, Jo, Rijal, S., J. AREVALO, Vandesompele, J., S. DE DONCKER, J. VANDESOMPELE, Yardley, V., Chappuis, F, Arevalo, J., Arevalo, J, Rijal, S, De Doncker, S., A. LLANOS-CUENTAS, Dujardin, JC, Decuypere, S   +31 more
core   +1 more source

Biochemical characterization and three-dimensional structure analysis of the yeast cleavage and polyadenylation factor CPF [PDF]

, 2008
In eukaryotes, protein-encoding genes are transcribed in the nucleus by RNA polymerase II (RNAP II). Before the gene transcript (pre-mRNA) is transported to the cytoplasm and can function in translation, it has to undergo three specific maturation ...
Paguet, Bertrand
core   +1 more source

Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3′ end biogenesis

eLife, 2022
3′ end formation of most eukaryotic mRNAs is dependent on the assembly of a ~1.5 MDa multiprotein complex, that catalyzes the coupled reaction of pre-mRNA cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF)
Lena Maria Muckenfuss, Anabel Carmen Migenda Herranz, Franziska Maria Boneberg, Marcello Clerici, Martin Jinek   +4 more
doaj   +1 more source

PAPD5, a noncanonical poly(A) polymerase with an unusual RNA-binding motif [PDF]

, 2011
PAPD5 is one of the seven members of the family of noncanonical poly(A) polymerases in human cells. PAPD5 was shown to polyadenylate aberrant pre-ribosomal RNAs in vivo, similar to degradation-mediating polyadenylation by the noncanonical poly(A ...
Bilen, B., Keller, W., Mihaela Zavolan, Biter Bilen, Zavolan, M., Walter Keller, Rammelt, C., Christiane Rammelt   +7 more
core   +1 more source