Results 121 to 130 of about 9,343 (180)
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Polyamine oxidase in rat tissues
Biochimica et Biophysica Acta (BBA) - Enzymology, 1980An assay procedure for polyamine oxidase in tissue homogenates was devised. The method is based on the degradation of N1,n12-diacetylspermine to N1-acetylspermidine and the determination using TLC of the latter. Polyamine oxidase activity is high in most tissues. Its activity is comparable to that of spermidine and spermine synthase.
N, Seiler +3 more
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Characterization of maize polyamine oxidase
Phytochemistry, 1990Some structural and biochemical characteristics of polyamine oxidase (PAO) purified from maize shoots have been examined. The enzyme has only alanine as N-terminal amino acid and its N-terminal sequence shows a significant degree of homology with tryptophan 2-monooxygenase from Pseudomonas syringae pv. savastanoi.
FEDERICO, RODOLFO +4 more
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Inhibition of polyamine and spermine oxidases by polyamine analogues
The FEBS Journal, 2006Polyamine oxidase (PAO) and spermine oxidase (SMO) are involved in the catabolism of polyamines – basic regulators of cell growth and proliferation. The discovery of selective inhibitors of PAO and SMO represents an important tool in studying the involvement of these enzymes in polyamine homeostasis and a starting point for the development of novel ...
Bianchi, M. +6 more
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Cytotoxicity of polyamines to Amoeba proteus: Role of polyamine oxidase
Cell Biology and Toxicology, 1996It has been shown that oxidation of polyamines by polyamine oxidases can produce toxic compounds (H2O2, aldehydes, ammonia) and that the polyamine oxidase-polyamine system is implicated, in vitro, in the death of several parasites. Using Amoeba proteus as an in vitro model, we studied the cytotoxicity to these cells of spermine, spermidine, their ...
Schenkel, Eric +3 more
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Polyamine Oxidases and Oxidized Polyamines
2021Polyamine oxidases that act at the secondary amino group would be further subdivided according to whether diaminopropane or aminopropionaldehyde were among the products. The highest specific activity was found in the peroxisomal fraction, where its presence has been confirmed histochemically in both rat liver and kidney, although polyamine oxidase was ...
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A radioisotopic assay for polyamine oxidase
Analytical Biochemistry, 1990A new radioisotopic assay for polyamine oxidase with N1-acetylspermine as substrate is presented. A modified method for the chemical synthesis of radioactive N1-acetylspermine, which gave a good yield, is also described. The reaction mixture, containing N1-[14C]acetylspermine and tissue homogenate, was incubated for the enzyme reaction and applied to a
T, Kumazawa, H, Seno, O, Suzuki
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Amino oxidase amperometric biosensor for polyamines
Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, 1991Abstract An improved amino oxidase enzyme electrode has been constructed and applied to the determination of the amount of polyamines present in real samples. The electrode is based on the amperometric detection of H 2 O 2 produced in the enzymatic oxidation of polyamines by amino oxidase.
GASPARINI R. +4 more
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Polyamine oxidase in higher plants
Biochemical and Biophysical Research Communications, 1970Abstract A polyamine-specific oxidase found in the leaves of barley and maize seedlings oxidises spermine optimally at pH 4.5, the products being hydrogen peroxide, 1-(3-aminopropyl) pyrroline and 1,3-diaminopropane. Spermidine and 3,3′-diaminodipropylamine were oxidised more slowly than spermine with an optimum at about pH 7.0.
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