Results 51 to 60 of about 15,660 (211)
Multifunctional polyketide synthase genes identified by genomic survey of the symbiotic dinoflagellate, Symbiodinium minutum [PDF]
, 2015 Table S1. Predicted domains from transcriptome contigs Figure S1. Expression of KS domain-containing genes on scaffolds of S. minutum. Read coverages of RNAseq (gray line) on KS domain-containing genes (surrounded by green) show expression in our ...
Eiichi Shoguchi +4 more
core +4 more sources
Nature Communications, 2021 Engineering efforts have focused on acyltransferase (AT) domains of modular polyketide synthases (PKSs) to site-selectively modify the resulting polyketides, but critical AT residues involved in substrate selection have not been fully elucidated.
Edward Kalkreuter +6 more
doaj +1 more source
Pass-back chain extension expands multimodular assembly line biosynthesis. [PDF]
, 2020 Modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymatic assembly lines are large and dynamic protein machines that generally effect a linear sequence of catalytic cycles.
Huan, Tao +5 more
core
Polyketide synthesis in vitro on a modular polyketide synthase
Chemistry & Biology, 1995 The 6-deoxyerythronolide B synthase (DEBS) of Saccharopolyspora erythraea, which synthesizes the aglycone core of the antibiotic erythromycin A, contains some 30 active sites distributed between three multienzyme polypeptides (designated DEBS1-3). This complexity has hitherto frustrated mechanistic analysis of such enzymes.
Wiesmann, Kirsten E.H. +5 more
openaire +2 more sources
Nature Communications, 2019 The wealth of complex polyketides is an essential source for drug discovery. Here, the authors report an evolution-guided rational morphing of modular polyketide synthases (PKSs) for aurothin and neoaurothin biosynthesis, and reveal engineering site ...
Huiyun Peng +4 more
doaj +1 more source
Zebrafish otolith biomineralization requires polyketide synthase
Mechanisms of Development, 2019 Deflecting biomineralized crystals attached to vestibular hair cells are necessary for maintaining balance. Zebrafish (Danio rerio) are useful organisms to study these biomineralized crystals called otoliths, as many required genes are homologous to human otoconial development.
Thiessen, Kevin D. +6 more
openaire +2 more sources
Next-generation sequencing approach for connecting secondary metabolites to biosynthetic gene clusters in fungi [PDF]
, 2015 Genomics has revolutionized the research on fungal secondary metabolite (SM) biosynthesis. To elucidate the molecular and enzymatic mechanisms underlying the biosynthesis of a specific SM compound, the important first step is often to find the genes that
Cacho, Ralph A. +2 more
core +1 more source
Structural control of polyketide formation in plant-specific polyketide synthases [PDF]
Chemistry & Biology, 2000 Polyketide synthases (PKSs) generate molecular diversity by utilizing different starter molecules and by controlling the final length of the polyketide. Although exploitation of this mechanistic variability has produced novel polyketides, the structural foundation of this versatility is unclear. Plant-specific PKSs are essential for the biosynthesis of
Jez, Joseph M +5 more
openaire +2 more sources
Phylogenomic analyses of non-Dikarya fungi supports horizontal gene transfer driving diversification of secondary metabolism in the amphibian gastrointestinal symbiont, Basidiobolus [PDF]
, 2020 Research into secondary metabolism (SM) production by fungi has resulted in the discovery of diverse, biologically active compounds with significant medicinal applications. However, the fungi rich in SM production are taxonomically restricted to Dikarya,
Chang, Ying +9 more
core +1 more source
Biosynthesis of polyketide synthase extender units [PDF]
Nat. Prod. Rep., 2009 This review covers the biosynthesis of extender units that are utilized for the assembly of polyketides by polyketide synthases. The metabolic origins of each of the currently known polyketide synthase extender units are covered.
Yolande A, Chan +3 more
openaire +2 more sources