Results 21 to 30 of about 27,763 (265)

Polyketide synthesis in vitro on a modular polyketide synthase

Chemistry & Biology, 1995
The 6-deoxyerythronolide B synthase (DEBS) of Saccharopolyspora erythraea, which synthesizes the aglycone core of the antibiotic erythromycin A, contains some 30 active sites distributed between three multienzyme polypeptides (designated DEBS1-3). This complexity has hitherto frustrated mechanistic analysis of such enzymes.
Wiesmann, Kirsten E.H., Cortés, Jesus, Brown, Murray J.B., Cutter, Annabel L., Staunton, James, Leadlay, Peter F.   +5 more
openaire   +2 more sources

MultiMetEval: comparative and multi-objective analysis of genome-scale metabolic models [PDF]

, 2012
Comparative metabolic modelling is emerging as a novel field, supported by the development of reliable and standardized approaches for constructing genome-scale metabolic models in high throughput.
A Freitag, A Gevorgyan, A Mithani, Albert Gevorgyan, AM Feist, AM Feist, AM Feist, Andrzej M. Kierzek, AP Burgard, BA Pfeifer, CS Henry, D Nagrath, DC Alexander, DC Stevens, EM Paradise, Eriko Takano, FC Lee, H Ikeda, I Thiele, J Gonzalez-Lergier, J Romero, J Schellenberger, JD Orth, JL Adrio, JP Brooks, JS Edwards, K Nieselt, K Yanai, LM Blank, M DeJongh, M Durot, M Kanehisa, M Latendresse, MA Fischbach, MA Oberhardt, MA Oberhardt, MA Oberhardt, Marnix H. Medema, MH Medema, MH Medema, MH Medema, MH Medema, MT Alam, MT Alam, MW Covert, N Scherr, ND Price, ND Price, NE Lewis, Piotr Zakrzewski, PR Ives, R Breitling, R Schuetz, Rainer Breitling, SA Becker, SD Bentley, Stephen S. Fong, TD Vo, TY Kim, XQ Zhao   +59 more
core   +6 more sources

Iterative polyketide biosynthesis by modular polyketide synthases in bacteria [PDF]

Applied Microbiology and Biotechnology, 2015
Modular polyketide synthases (type I PKSs) in bacteria are responsible for synthesizing a significant percentage of bioactive natural products. This group of synthases has a characteristic modular organization, and each module within a PKS carries out one cycle of polyketide chain elongation; thus each module is non-iterative in function.
Haotong, Chen, Liangcheng, Du
openaire   +2 more sources

Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening [PDF]

, 2003
New secondary mesenchyme specific genes, expressed exclusively in pigment cells, were isolated from sea urchin embryos using a differential screening of a macroarray cDNA library. The comparison was performed between mRNA populations of embryos having an
Calestani, Cristina, Davidson, Eric H., Rast, Jonathan P.   +2 more
core   +1 more source

A Polyketide Synthase Component for Oxygen Insertion into Polyketide Backbones [PDF]

Angewandte Chemie International Edition, 2018
AbstractEnzymatic core components from trans‐acyltransferase polyketide synthases (trans‐AT PKSs) catalyze exceptionally diverse biosynthetic transformations to generate structurally complex bioactive compounds. Here we focus on a group of oxygenases identified in various trans‐AT PKS pathways, including those for pederin, oocydins, and toblerols ...
Roy A. Meoded, Reiko Ueoka, Eric J. N. Helfrich, Katja Jensen, Nancy Magnus, Birgit Piechulla, Jörn Piel   +6 more
openaire   +5 more sources

Sampling Terrestrial Environments for Bacterial Polyketides

Molecules, 2017
Bacterial polyketides are highly biologically active molecules that are frequently used as drugs, particularly as antibiotics and anticancer agents, thus the discovery of new polyketides is of major interest.
Patrick Hill, Graham W. Heberlig, Christopher N. Boddy   +2 more
doaj   +1 more source

Machine Learning Accelerates Crystallization for Structure Determination

Angewandte Chemie, EarlyView.
Single‐crystal X‐ray diffraction (SCXRD) is often constrained by the difficulty of obtaining suitable crystals. Here, a machine learning‐accelerated co‐crystal discovery workflow is established for a crystalline mate strategy that achieves over 95% prediction accuracy and experimentally delivers 114 co‐crystals from 120 candidates.
Cui‐Zhou Luan, Xue‐Zhi Wang, Jian‐Guo Song, Yu Gu, Jing Wu, Ye‐Ting Wang, Jin‐Feng Liang, Jia‐Le Rao, Mo Xie, Jonathan R. Nitschke, Dan Li   +10 more
wiley   +2 more sources

Chemical probes for the functionalization of polyketide intermediates [PDF]

, 2014
A library of functionalized chemical probes capable of reacting with ketosynthase-bound biosynthetic intermediates was prepared and utilized to explore in vivo polyketide diversification. Fermentation of ACP mutants of S.
Abdulla, Adovelande, Bhatt, Böhrsch, Carter, Cortes, Cummings, Dutta, Goss, Grammel, Gupta, Huczynski, Hüttel, Kirschning, Knobloch, Koryakina, Kushnir, Laureti, Lyazghi, Newman, Ojima, Oliynyk, Purser, Rostovtsev, Rowe, Schilling, Schmidt, Schmidt, Serwa, Sletten, Sundermann, Tosin, Tosin, Tosin, Tosin, Walker, Walker, Wang, Weissman, Whicher, Winter   +40 more
core   +7 more sources

New Polyketides from Mangrove Endophytic Fungus Penicillium sp. BJR-P2 and Their Anti-Inflammatory Activity

Marine Drugs, 2022
Four new polyketide compounds, including two new unique isocoumarins penicillol A (1) and penicillol B (2) featuring with spiroketal rings, two new citreoviridin derivatives citreoviridin H (3) and citreoviridin I (4), along with four known analogues ...
Chen Chen, Geting Ye, Jing Tang, Jialin Li, Wenbin Liu, Li Wu, Yuhua Long   +6 more
doaj   +1 more source

Assessment of extracts from red yeast rice for herb-drug interaction by in-vitro and in-vivo assays [PDF]

, 2012
Red yeast rice (RYR) is made by fermenting the yeast Monascus purpureus over rice. It is a source of natural red food colorants, a food garnish and a traditional medication.
Fung, W.T., Subramaniam, G., Lee, J., Loh, H.M., Leung, P.H.H.   +4 more
core   +2 more sources