Promiscuity‐Guided Enzyme Evolution via Substrate Multiplexed Screening
Angewandte Chemie, EarlyView.Through historical context and recent examples, this Minireview summarizes the strengths, limitations, and practical considerations for the implementation of substrate multiplexed screening (SUMS) in enzyme evolution. ABSTRACT An enzyme's ability to react with diverse substrates while maintaining high yield and selectivity, termed “substrate ...
Holly A. Weilbaker +3 more
wiley +2 more sources
Recently Discovered Secondary Metabolites from Streptomyces Species
Molecules, 2022 The Streptomyces genus has been a rich source of bioactive natural products, medicinal chemicals, and novel drug leads for three-quarters of a century. Yet studies suggest that the genus is capable of making some 150,000 more bioactive compounds than all
Heather J. Lacey, Peter J. Rutledge
doaj +1 more source
Inferring Chemical Reaction Patterns Using Rule Composition in Graph Grammars [PDF]
, 2012 Modeling molecules as undirected graphs and chemical reactions as graph rewriting operations is a natural and convenient approach tom odeling chemistry. Graph grammar rules are most naturally employed to model elementary reactions like merging, splitting,
Andersen, Jakob L. +3 more
core +3 more sources
Antimicrobial Resistance: We Must Pursue a Collaborative, Global Approach and Use a "One Health" Approach. [PDF]
, 2019 Treating infection is a key part of the work of most clinicians [...]
Lipman, J, Lonsdale, DO
core +1 more source
The Genetic Basis of Toxin Biosynthesis in Dinoflagellates
Microorganisms, 2019 In marine ecosystems, dinoflagellates can become highly abundant and even dominant at times, despite their comparatively slow growth rates. One factor that may play a role in their ecological success is the production of complex secondary metabolite ...
Arjun Verma +5 more
doaj +1 more source
Simultaneous Effect of Temperature and Irradiance on Growth and Okadaic Acid Production from the Marine Dinoflagellate Prorocentrum belizeanum [PDF]
, 2014 Benthic marine dioflagellate microalgae belonging to the genus Prorocentrum are a major source of okadaic acid (OA), OA analogues and polyketides. However, dinoflagellates produce these valuable toxins and bioactives in tiny quantities, and they grow ...
Belarbi, El Hassan +6 more
core +3 more sources
Reconstruction of the biosynthetic pathway for the core fungal polyketide scaffold rubrofusarin in Saccharomyces cerevisiae [PDF]
, 2013 BACKGROUND: Fungal polyketides include commercially important pharmaceuticals and food additives, e.g. the cholesterol-lowering statins and the red and orange monascus pigments.
Frandsen, Rasmus John Normand +3 more
core +1 more source
Characterization of Eight Novel Spiroleptosphols from Fusarium avenaceum
Molecules, 2019 Chemical analyses of Fusarium avenaceum grown on banana medium resulted in eight novel spiroleptosphols, T1, T2 and U−Z (1−8). The structures were elucidated by a combination of high-resolution mass spectrometric data and 1- and 2-D NMR ...
Klaus Ringsborg Westphal +11 more
doaj +1 more source
Engineered polyketides: Synergy between protein and host level engineering [PDF]
, 2017 Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities.
Bailey, Constance B. +4 more
core +2 more sources
Manipulating polyketide stereochemistry by exchange of polyketide synthase modules
Chemical Communications, 2020 Exchange of polyketide synthase (PKS) modules by genetic engineering leads to efficient modification of polyketide stereochemistry.
Massicard, Jean-Malo +3 more
openaire +4 more sources