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Polymerase chain displacement reaction
BioTechniques, 2013 Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris +4 more
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A portable low-cost polymerase chain reaction device [PDF]
HardwareXPolymerase chain reaction (PCR) is a cornerstone technique in molecular biology and clinical diagnostics. However, conventional PCR systems are often bulky and prohibitively expensive, limiting their use in resource-limited settings.
Kan Luo +6 more
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Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]
Veterinary World, 2021 Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid +5 more
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Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Archives of Razi Institute, 2020 Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
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Polymerase Chain Reaction [PDF]
Journal of Investigative Dermatology, 2013 The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, 1990). For the first time, it allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project.
Garibyan, Lilit, Avashia, Nidhi
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Mediterranean Journal of Infection, Microbes and Antimicrobials, 2023 Introduction: Coronavirus disease-2019 (COVID-19) has affected more than 676 million people till date, and 6,881,955 people have died from the disease as of March 28, 2023.
Müge TOYGAR DENİZ +3 more
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Optimisation of microfluidic polymerase chain reaction devices [PDF]
E3S Web of Conferences, 2021 The invention and development of Polymerase Chain Reaction (PCR) technology have revolutionised molecular biology and molecular diagnostics. There is an urgent need to optimise the performance of these devices while reducing the total construction and ...
Zagklavara Foteini +4 more
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International Journal of Mycobacteriology, 2022 Background: In this study, it was aimed to investigate Mycobacterium bovis strains isolated from lungs and lymph nodes of slaughtered animals on clonal level by using different methods such as spoligotyping, enterobacterial repetitive intergenic ...
Funda Sahin +4 more
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Polymerase Chain Reaction [PDF]
Cold Spring Harbor Protocols, 2019 The polymerase chain reaction (PCR) underlies almost all of modern molecular cloning. Using PCR, a defined target sequence that occurs once within a DNA of high complexity and large size—an entire mammalian genome, for example—can be rapidly and selectively amplified in a quasi-exponential chain reaction that generates millions of copies.
Michael R, Green, Joseph, Sambrook
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Polymerase chain reaction [PDF]
BMJ, 1997 The polymerase chain reaction (PCR) was devised just over a decade ago, yet it is already an integral part of much biological and medical research. A glance at current journal articles shows that it is also being used to develop new diagnostic tests, which are already having an impact on clinical practice.
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