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The polymerase chain reaction [PDF]
Trends in Genetics, 1989 The polymerase chain reaction (PCR) is a powerful new method for 'in vitro cloning'. It can selectively amplify a single molecule of template DNA several millionfold in a few hours and has made possible new approaches to problems in molecular genetics, evolutionary biology, and development.
Henry A. Erlich +2 more
semanticscholar +12 more sources
Polymerase chain displacement reaction
BioTechniques, 2013 Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris +4 more
doaj +4 more sources
Polymerase chain reaction fingerprints [PDF]
Nucleic Acids Research, 1987 ROLLO, Franco Ugo +3 more
openaire +6 more sources
Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Archives of Razi Institute, 2020 Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
doaj +1 more source
Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]
Veterinary World, 2021 Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid +5 more
doaj +1 more source
Polymerase Chain Reaction [PDF]
Journal of Investigative Dermatology, 2013 The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, 1990). For the first time, it allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project.
Nidhi Avashia, Lilit Garibyan
openaire +5 more sources
Mediterranean Journal of Infection, Microbes and Antimicrobials, 2023 Introduction: Coronavirus disease-2019 (COVID-19) has affected more than 676 million people till date, and 6,881,955 people have died from the disease as of March 28, 2023.
Müge TOYGAR DENİZ +3 more
doaj +1 more source
Optimisation of microfluidic polymerase chain reaction devices [PDF]
E3S Web of Conferences, 2021 The invention and development of Polymerase Chain Reaction (PCR) technology have revolutionised molecular biology and molecular diagnostics. There is an urgent need to optimise the performance of these devices while reducing the total construction and ...
Zagklavara Foteini +4 more
doaj +1 more source
International Journal of Mycobacteriology, 2022 Background: In this study, it was aimed to investigate Mycobacterium bovis strains isolated from lungs and lymph nodes of slaughtered animals on clonal level by using different methods such as spoligotyping, enterobacterial repetitive intergenic ...
Funda Sahin +4 more
doaj +1 more source
Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]
Veterinary World, 2017 Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal +6 more
doaj +1 more source