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The polymerase chain reaction. [PDF]
Trends in Genetics, 1989 The polymerase chain reaction (PCR) is a powerful new method for 'in vitro cloning'. It can selectively amplify a single molecule of template DNA several millionfold in a few hours and has made possible new approaches to problems in molecular genetics, evolutionary biology, and development.
I R Peake
semanticscholar +12 more sources
DNA extraction and polymerase chain reaction
Journal of Cytology, 2019 DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
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A portable low-cost polymerase chain reaction device [PDF]
HardwareXPolymerase chain reaction (PCR) is a cornerstone technique in molecular biology and clinical diagnostics. However, conventional PCR systems are often bulky and prohibitively expensive, limiting their use in resource-limited settings.
Kan Luo +6 more
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SSRN Electronic Journal, 2020 The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a ...
Shomaila Sikandar +3 more
semanticscholar +8 more sources
Polymerase chain displacement reaction
BioTechniques, 2013 Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris +4 more
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Polymerase chain reaction fingerprints [PDF]
Nucleic Acids Research, 1987 Franco Rollo +3 more
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Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Archives of Razi Institute, 2020 Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
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Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]
Veterinary World, 2021 Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid +5 more
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Mediterranean Journal of Infection, Microbes and Antimicrobials, 2023 Introduction: Coronavirus disease-2019 (COVID-19) has affected more than 676 million people till date, and 6,881,955 people have died from the disease as of March 28, 2023.
Müge TOYGAR DENİZ +3 more
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Optimisation of microfluidic polymerase chain reaction devices [PDF]
E3S Web of Conferences, 2021 The invention and development of Polymerase Chain Reaction (PCR) technology have revolutionised molecular biology and molecular diagnostics. There is an urgent need to optimise the performance of these devices while reducing the total construction and ...
Zagklavara Foteini +4 more
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