Results 211 to 220 of about 218,617 (259)
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POLYMERASE CHAIN REACTION STRATEGY
Annual Review of Biochemistry, 1992INTRODUCTION ..... . . . . . . . . . .. . . . . . . .... . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . .... . . . .. . ..... . .. . . THE BASIC METHOD . . .. . . . . . . . . . . ... .. . . . . . . . . . ..... . . . . . . . . . . ..... . . . . . . . . . . .... . . . . . . . . . . . . . . . . BIOCHEMISTRY OF THE REACTION .
N, Arnheim, H, Erlich
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Journal of Clinical Immunology, 1989
The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by ...
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The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by ...
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Introduction to the Polymerase Chain Reaction
2006The polymerase chain reaction (PCR) is an in vitro method for the amplification of DNA. Since the introduction of the PCR in 1985, it has become an indispensable technique for many applications in scientific research and clinical and forensic investigations.
Y M Dennis, Lo, K C Allen, Chan
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The polymerase chain reaction in histopathology
Histopathology, 1995Thanks to the advent of the polymerase chain reaction (PCR) molecular genetic study of histological samples is now a relatively straightforward task and the vast histopathology archives are now open to molecular analysis. In this review we outline technical aspects of PCR analysis of histological material and evaluate its application to the diagnosis ...
L X, Pan, T C, Diss, P G, Isaacson
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The Journal of Dermatologic Surgery and Oncology, 1993
Within the space of the last 5 years, application of the revolutionary in vitro method of deoxyribonucleic acid (DNA) amplification known as the polymerase chain reaction (PCR), has become ubiquitous. The rapidly increasing number of clinical and research articles utilizing this technology, both in the dermatologic and general medical literature ...
J L, Snow, K, Snow, M R, Pittelkow
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Within the space of the last 5 years, application of the revolutionary in vitro method of deoxyribonucleic acid (DNA) amplification known as the polymerase chain reaction (PCR), has become ubiquitous. The rapidly increasing number of clinical and research articles utilizing this technology, both in the dermatologic and general medical literature ...
J L, Snow, K, Snow, M R, Pittelkow
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Inverse Polymerase Chain Reaction
Nature Biotechnology, 1989We describe a procedure that extends the utility of the polymerase chain reaction (PCR) in allowing the geometric amplification of an unknown DNA sequence that flanks a core region of known sequence. DNA containing the core region is digested with appropriate restriction enzymes to produce a fragment of suitable size for PCR amplification.
H, Ochman +3 more
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1990
The development of a new technology can lead to novel approaches to answering current scientific questions. A new technology can also often lead to the framing of scientific questions that previously were considered intractable and not amenable to experimental investigation.
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The development of a new technology can lead to novel approaches to answering current scientific questions. A new technology can also often lead to the framing of scientific questions that previously were considered intractable and not amenable to experimental investigation.
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Polymerase chain reaction engineering
Biotechnology and Bioengineering, 1997A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kinetic model to get a better understanding of the ...
J T, Hsu, S, Das, S, Mohapatra
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Polymerase Chain Reaction on Microchips
2006The polymerase chain reaction (PCR) provides an in vitro method for rapid enzymatic amplification of fragments of DNA. Microchip-based PCR devices (with reaction volumes from picoliters to microliters) have been realized using various combinations of silicon, glass, and/or plastic materials.
Maria C, Carles, Nikolaus J, Sucher
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Polymerase Chain Reaction and HIV
Clinics in Laboratory Medicine, 1994In this article, the synergistic progress for both polymerase chain reaction (PCR) technology and HIV-1 is reviewed. The latest developments in standard PCR technology for the detection and quantitation of HIV-1 DNA molecules are summarized. In addition, examples of applications selected from the fields of HIV-1 diagnosis, epidemiology, pathogenesis ...
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