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Polymerase Chain Reaction: Expedition of a ubiquitous tool
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The polymerase chain reaction (PCR) has had a significant impact on all aspects of the molecular biosciences, from cancer research to forensic science. The sensitivity and specificity inherent in the technique allow minute quantities of genetic material to be detected while the unique properties of thermostable DNA polymerase ensure that abundant ...
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Trends in Genetics, 1989
The polymerase chain reaction (PCR) is a powerful new method for 'in vitro cloning'. It can selectively amplify a single molecule of template DNA several millionfold in a few hours and has made possible new approaches to problems in molecular genetics, evolutionary biology, and development.
T J, White, N, Arnheim, H A, Erlich
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The polymerase chain reaction (PCR) is a powerful new method for 'in vitro cloning'. It can selectively amplify a single molecule of template DNA several millionfold in a few hours and has made possible new approaches to problems in molecular genetics, evolutionary biology, and development.
T J, White, N, Arnheim, H A, Erlich
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Current Protocols in Protein Science, 2002
AbstractThis appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Martha F, Kramer, Donald M, Coen
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AbstractThis appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Martha F, Kramer, Donald M, Coen
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Journal of Infectious Diseases, 1988
organism number to readily detectable levels, yet culture is not always easy or successful. A novel technique, the polymerase chain reaction (PCR), was recently developed for in vitro amplification of the DNA or RNA of an organism or gene defect, and culture may not be required.
G, Schochetman, C Y, Ou, W K, Jones
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organism number to readily detectable levels, yet culture is not always easy or successful. A novel technique, the polymerase chain reaction (PCR), was recently developed for in vitro amplification of the DNA or RNA of an organism or gene defect, and culture may not be required.
G, Schochetman, C Y, Ou, W K, Jones
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Modeling the Polymerase Chain Reaction
Journal of Computational Biology, 1995We introduce a mathematical model to treat the polymerase chain reaction (PCR), where we regard the accumulation of new molecules during a PCR cycle as a randomly bifurcating tree. This model enables us to compute an approximate formula for the distribution of the number of replications that have occurred between a pair of molecules, which depends on ...
Gunter Weiss, Arndt von Haeseler
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Clinics in Dermatology, 1991
In a short time the PCR techniques has revolutionized research technology in many areas of medicine. Because of the ease and rapidity of the technique it is quickly becoming a standard clinical test for many diseases. Clinical applications continue to emerge from research labs and should rapidly expand to facilitate rapid medical diagnosis.
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In a short time the PCR techniques has revolutionized research technology in many areas of medicine. Because of the ease and rapidity of the technique it is quickly becoming a standard clinical test for many diseases. Clinical applications continue to emerge from research labs and should rapidly expand to facilitate rapid medical diagnosis.
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Polymerase Chain Reaction in Gastroenterology
Journal of Clinical Gastroenterology, 1993Polymerase chain reaction (PCR) has had a major impact on molecular biology and is beginning to change our perception of certain disease processes and diagnostic tests. PCR allows the identification and amplification of very rare nucleic acid sequences.
P H, Hayashi, J B, Zeldis
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