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Immunocapture-Polymerase Chain Reaction
2005Antibody capture of viruses can be used as a preparatory step in nucleic acid amplification techniques. Immunocapture of virus particles can be used to streamline and/or optimize the concentration, purification and specificity requirements of polymerase chain reaction assays.
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Introduction to Polymerase Chain Reaction
2003The polymerase chain reaction (PCR) has become an indispensable tool of molecular biology (1-5). Since its discovery in 1985 the process has found its integration into all research areas involving the use of DNA and RNA. Using this technique, a small starting sample of DNA or RNA can be used to amplify a specific DNA or RNA target over a million-fold ...
M C, Miller, L, Cunningham
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Polymerase chain reaction techniques
Current Opinion in Biotechnology, 1991Abstract Recent improvements to the polymerase chain reaction have produced greater priming specificity, better methods for the isolation of unknown DNA sequences, more efficient DNA recovery techniques, and new approaches to mutation detection and oligonucleotide synthesis.
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PCR – the polymerase chain reaction
Analytical Methods, 2014DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. Developed in 1985 by Kary B. Mullis, PCR revolutionized the way that deoxyribonucleic acid (DNA) could be copied.
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Introduction to the Polymerase Chain Reaction
2003The polymerase chain reaction (PCR) is an in vitro method for the amplification of DNA that was introduced in 1985 (1). The principle of the PCR is elegantly simple but the resulting method is extremely powerful. The adoption of the thermostable Taq polymerase in 1988 greatly simplifies the process and enables the automatron of PCR (2).
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Selection of Primers for Polymerase Chain Reaction
Molecular Biotechnology, 1995One of the most important factors affecting the quality of PCR is the choice of primers. In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the chance of finding an optimal primer pair.
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The Polymerase Chain Reaction and Branching Processes
Journal of Computational Biology, 1995We construct a mathematical model for the polymerase chain reaction and its mutations using the theory of branching processes. Under this model we study the number of mutations in a randomly chosen sequence after n PCR cycles. A method for estimating the mutation is proposed and the variance of this estimator is studied.
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Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction
2009Polymerase chain reaction (PCR) enables one to determine if a specific needle is present in a haystack, and it can be used as a step toward the characterization of the needle. It is a quick, powerful, inexpensive DNA amplification technique that has become a fundamental tool in molecular pathology.
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Understanding the Polymerase Chain Reaction
Transfusion Medicine Reviews, 1994H, Ni, M A, Blajchman
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