Results 221 to 230 of about 861,684 (262)

Polymerase Chain Reaction and HIV

Clinics in Laboratory Medicine, 1994
In this article, the synergistic progress for both polymerase chain reaction (PCR) technology and HIV-1 is reviewed. The latest developments in standard PCR technology for the detection and quantitation of HIV-1 DNA molecules are summarized. In addition, examples of applications selected from the fields of HIV-1 diagnosis, epidemiology, pathogenesis ...
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Polymerase Chain Reaction on Microchips

2006
The polymerase chain reaction (PCR) provides an in vitro method for rapid enzymatic amplification of fragments of DNA. Microchip-based PCR devices (with reaction volumes from picoliters to microliters) have been realized using various combinations of silicon, glass, and/or plastic materials.
Maria C, Carles, Nikolaus J, Sucher
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Polymerase chain reaction engineering

Biotechnology and Bioengineering, 1997
A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kinetic model to get a better understanding of the ...
J T, Hsu, S, Das, S, Mohapatra
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Introduction to Polymerase Chain Reaction

2003
The polymerase chain reaction (PCR) has become an indispensable tool of molecular biology (1-5). Since its discovery in 1985 the process has found its integration into all research areas involving the use of DNA and RNA. Using this technique, a small starting sample of DNA or RNA can be used to amplify a specific DNA or RNA target over a million-fold ...
M C, Miller, L, Cunningham
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Polymerase chain reaction techniques

Current Opinion in Biotechnology, 1991
Abstract Recent improvements to the polymerase chain reaction have produced greater priming specificity, better methods for the isolation of unknown DNA sequences, more efficient DNA recovery techniques, and new approaches to mutation detection and oligonucleotide synthesis.
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Immunocapture-Polymerase Chain Reaction

2005
Antibody capture of viruses can be used as a preparatory step in nucleic acid amplification techniques. Immunocapture of virus particles can be used to streamline and/or optimize the concentration, purification and specificity requirements of polymerase chain reaction assays.
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PCR – the polymerase chain reaction

Analytical Methods, 2014
DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. Developed in 1985 by Kary B. Mullis, PCR revolutionized the way that deoxyribonucleic acid (DNA) could be copied.
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Introduction to the Polymerase Chain Reaction

2003
The polymerase chain reaction (PCR) is an in vitro method for the amplification of DNA that was introduced in 1985 (1). The principle of the PCR is elegantly simple but the resulting method is extremely powerful. The adoption of the thermostable Taq polymerase in 1988 greatly simplifies the process and enables the automatron of PCR (2).
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Selection of Primers for Polymerase Chain Reaction

Molecular Biotechnology, 1995
One of the most important factors affecting the quality of PCR is the choice of primers. In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the chance of finding an optimal primer pair.
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Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction

2009
Polymerase chain reaction (PCR) enables one to determine if a specific needle is present in a haystack, and it can be used as a step toward the characterization of the needle. It is a quick, powerful, inexpensive DNA amplification technique that has become a fundamental tool in molecular pathology.
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