Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping [PDF]
The Kidd blood group antigens, Jkª and Jkᵇ , are two of the main surface markers which are found on the membrane of red blood cells. The determination of whether a donor or a recipient has the Jkª and/or the Jkᵇ antigens is crucially important to ...
Borg, Joseph J.+2 more
core +1 more source
Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription [PDF]
Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to ...
Baltimore, David+2 more
core +5 more sources
Computing by polymerase chain reaction [PDF]
A mathematical notation is introduced to represent, at a symbolic level, different mechanisms of DNA recombination, and a 'PCR lemma' is proven by analytically describing the combinatorial properties of the polymerase chain reaction process. This approach led to the discovery of novel techniques, based on a form of PCR which we called cross pairing PCR
MANCA, Vincenzo, FRANCO, Giuditta
openaire +3 more sources
Laboratory Detection of Novel Corona Virus 2019 Using Polymerase Chain Reaction [PDF]
Since mid-December 2019, several cases of a pneumonia like disease (with symptoms including fever, difficulty in breathing, cough and invasive lesions on both lungs) of unknown cause have emerged in the central Chinese city of Wuhan.
Chudhary, S. A. (Shafiq)+2 more
core
Efficiency of the polymerase chain reaction [PDF]
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology such as gene expression studies, mutation detection, forensic analysis and pathogen detection. Increasingly quantitative real time PCR is used to assess copy numbers from overall yield.
Booth, Christine S.+5 more
openaire +3 more sources
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj +1 more source
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke+33 more
core +1 more source
Rickettsia prowazekii and Real-time Polymerase Chain Reaction
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka+4 more
doaj +1 more source
Comparison of PCR fingerprinting techniques for the discrimination of Salmonella enterica subsp. enterica serovar Weltevreden isolated from indigenous vegetables in Malaysia. [PDF]
Salmonella enterica subsp. enterica (S.) serovar Weltevreden has emerged as a public health problem in many countries. Genomic DNA of S. Weltevreden from indigenous vegetables namely 'selom' (Oenanthe stolonifera), 'pegaga' (Centella asiatica), 'kesum ...
Awang Saleh, Noorzaleha+5 more
core +1 more source
Effects of upconversion nanoparticles on polymerase chain reaction. [PDF]
Nanoparticles (NPs) are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications.
Sang-Hyun Hwang+8 more
doaj +1 more source