Results 31 to 40 of about 1,395,900 (346)

Rickettsia prowazekii and Real-time Polymerase Chain Reaction

Emerging Infectious Diseases, 2006
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka, Jean-Marc Rolain, Yassina Bechah, John Gatabazi, Didier Raoult   +4 more
doaj   +1 more source

Efficiency of the polymerase chain reaction [PDF]

Chemical Engineering Science, 2010
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology such as gene expression studies, mutation detection, forensic analysis and pathogen detection. Increasingly quantitative real time PCR is used to assess copy numbers from overall yield.
Booth, Christine S., Pienaar, Elsje, Termaat, Joel R., Whitney, Scott E., Louw, Tobias M., Vijoen, Hendrik J.   +5 more
openaire   +3 more sources

Introduction of Digital Polymerase Chain Reaction: Comparison with Real-time Polymerase Chain Reaction

Korean Journal of Clinical Laboratory Science
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj   +1 more source

Effects of upconversion nanoparticles on polymerase chain reaction. [PDF]

PLoS ONE, 2013
Nanoparticles (NPs) are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications.
Sang-Hyun Hwang, Su-Gyeong Im, Sang Soo Hah, Vu Thanh Cong, Eun Jeong Lee, Yeon-Su Lee, Geon Kook Lee, Do-Hoon Lee, Sang Jun Son   +8 more
doaj   +1 more source

RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction]

Jurnal Teknologi dan Industri Pangan, 2014
Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp.
Ulfah Amalia, Ratih Dewanti-Hariyadi*, Achmad Poernomo   +2 more
doaj   +1 more source

The polymerase chain reaction

Advances in Physiology Education, 2004
This essay on the polymerase chain reaction is one of a series developed as part of FASEB’S efforts to educate the general public, and the legislators whom it elects, about the benefits of fundamental biomedical research—particularly how investment in such research leads to scientific progress, improved health, and economic well-being. 1
openaire   +3 more sources

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Polymerase chain reaction in microfluidic devices.

Lab on a Chip, 2016
The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude.
Christian D. Ahrberg, A. Manz, B. Chung
semanticscholar   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

The nicotinamide hypothesis revisited—plant defense signaling integrating PARP, nicotinamide, nicotinic acid, epigenetics, and glutathione

FEBS Letters, EarlyView.
Nicotinamide (NIC) and nicotinic acid (NIA) are proposed as stress signaling compounds in plants. Oxidative stress may lead to single strand breaks (SSB) in DNA, which activate poly(ADP‐ribose) polymerase (PARP). NIC and NIA are then formed from NAD. NIC and NIA can promote epigenetic changes leading to the expression of defense genes specific for the ...
Torkel Berglund, Anna B. Ohlsson
wiley   +1 more source