Results 31 to 40 of about 1,450,683 (312)
Robust design optimisation of continuous flow polymerase chain reaction thermal flow systems [PDF]
arXiv, 2021 This paper presents an efficient methodology for the robust optimisation of Continuous Flow Polymerase Chain Reaction (CFPCR) devices. It enables the effects of uncertainties in device geometry, due to manufacturing tolerances, on the competing objectives of minimising the temperature deviations within the CFPCR thermal zones, together with minimising ...
arxiv
Korean Journal of Clinical Laboratory ScienceDigital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj +1 more source
Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins [PDF]
Journal of Molecular Biology 350(4):806-816 (2005)., 2004 Recently, several groups have used error-prone polymerase chain reactions to construct mutant libraries containing up to 27 nucleotide mutations per gene on average, and reported a striking observation: although retention of protein function initially declines exponentially with mutations as has previously been observed, orders of magnitude more ...
arxiv +1 more source
Effects of upconversion nanoparticles on polymerase chain reaction. [PDF]
PLoS ONE, 2013 Nanoparticles (NPs) are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications.
Sang-Hyun Hwang +8 more
doaj +1 more source
Semiquantitative Group Testing in at Most Two Rounds [PDF]
arXiv, 2021 Semiquantitative group testing (SQGT) is a pooling method in which the test outcomes represent bounded intervals for the number of defectives. Alternatively, it may be viewed as an adder channel with quantized outputs. SQGT represents a natural choice for Covid-19 group testing as it allows for a straightforward interpretation of the cycle threshold ...
arxiv
Jurnal Teknologi dan Industri Pangan, 2014 Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp.
Ulfah Amalia +2 more
doaj +1 more source
Polymerase chain reaction in microfluidic devices.
Lab on a Chip, 2016 The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude.
Christian D. Ahrberg, A. Manz, B. Chung
semanticscholar +1 more source
Human colon organoid differentiation from induced pluripotent stem cells using an improved method
FEBS Letters, Volume 599, Issue 6, Page 912-924, March 2025.We compared established protocols to differentiate induced pluripotent stem cells into colon organoids and found that activation of bone morphogenetic protein 2 signaling induced gene expression of colon‐specific markers. Adenovirus‐mediated transduction of the transcription factors HOXD13 or SATB2 further enhanced colonic gene expression, suggesting ...
I‐Ting Lee +4 more
wiley +1 more source
A simple Bayesian model to estimate proportions and ratios from count data with a hierarchical error structure with an application to droplet digital PCR experiments [PDF]
arXiv, 2023 Experimental designs with hierarchically-structured errors are pervasive in many biomedical areas; it is important to take into account this hierarchical architecture in order to account for the dispersion and make reliable inferences from the data. This paper addresses the question of estimating a proportion or a ratio from positive or negative count ...
arxiv
Quantification Strategies in Real-time Polymerase Chain Reaction
Polymerase Chain Reaction: Theory and Technology, 2019 The present chapter describes the quantification strategies used in real-time RT-PCR (RT-qPCR), focusing on the main elements that are essential to fulfil the MIQE guidelines.
M. Pfaffl
semanticscholar +1 more source