Results 61 to 70 of about 1,450,683 (312)

Efficiency of DNA replication in the Polymerase Chain Reactio [PDF]

Proceedings of the National Academy of Sciences Volume 93, Number 23; Pages 12947-12952, November 12, 1996, 2001
We present a detailed kinetic model for the Polymerase Chain Reaction, and model the probability of replication in terms of the physical parameters of the problem. Applying the theory of branching processes, we show the existance of a new phenomenon affecting the probability distribution function of the number of replicants: for small number of initial
arxiv  

Dynamics and Control of DNA Sequence Amplification [PDF]

, 2014
DNA amplification is the process of replication of a specified DNA sequence \emph{in vitro} through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first ...
arxiv   +1 more source

Circulating tumor cells: advancing personalized therapy in small cell lung cancer patients

Molecular Oncology, EarlyView.
Small cell lung cancer (SCLC) is an aggressive form of lung cancer that spreads rapidly to secondary sites such as the brain and liver. Cancer cells circulating in the blood, “circulating tumor cells” (CTCs), have demonstrated prognostic value in SCLC, and evaluating biomarkers on CTCs could guide treatment decisions such as for PARP inhibitors ...
Prajwol Shrestha, Steven Kao, Veronica K. Cheung, Wendy A. Cooper, Nico van Zandwijk, John E. J. Rasko, Dannel Yeo   +6 more
wiley   +1 more source

Enhancing the specificity and efficiency of polymerase chain reaction using polyethyleneimine-based derivatives and hybrid nanocomposites

International Journal of Nanomedicine, 2012
Weiwei Tong1,2, Xueyan Cao2, Shihui Wen2, Rui Guo2, Mingwu Shen2, Jianhua Wang3, Xiangyang Shi1,2,41State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 2College of Chemistry, Chemical Engineering ...
Tong W, Cao X, Wen S, Guo R, Shen M, Wang J, Shi X   +6 more
doaj  

Cell‐free and extracellular vesicle microRNAs with clinical utility for solid tumors

Molecular Oncology, EarlyView.
Cell‐free microRNAs (cfmiRs) are small‐RNA circulating molecules detectable in almost all body biofluids. Innovative technologies have improved the application of cfmiRs to oncology, with a focus on clinical needs for different solid tumors, but with emphasis on diagnosis, prognosis, cancer recurrence, as well as treatment monitoring.
Yoshinori Hayashi, Janelle‐Cheri Millen, Romela Irene Ramos, Jennifer A. Linehan, Timothy G. Wilson, Dave S. B. Hoon, Matias A. Bustos   +6 more
wiley   +1 more source

Water Bridging Dynamics of Polymerase Chain Reaction in the Gauge Theory Paradigm of Quantum Fields [PDF]

Water 2017, 9, 339, 2018
We discuss the role of water bridging the DNA-enzyme interaction by resorting to recent results showing that London dispersion forces between delocalized electrons of base pairs of DNA are responsible for the formation of dipole modes that can be recognized by \textit{Taq} polymerase.
arxiv  

Randomized Trial of Rapid Multiplex Polymerase Chain Reaction-Based Blood Culture Identification and Susceptibility Testing.

Clinical Infectious Diseases, 2015
BACKGROUND The value of rapid, panel-based molecular diagnostics for positive blood culture bottles (BCBs) has not been rigorously assessed. We performed a prospective randomized controlled trial evaluating outcomes associated with rapid multiplex PCR ...
R. Banerjee, C. Teng, S. Cunningham, Sherry M. Ihde, J. Steckelberg, J. Moriarty, N. Shah, J. Mandrekar, Robin Patel   +8 more
semanticscholar   +1 more source

Unraveling the metastasis‐preventing effect of miR‐200c in vitro and in vivo

Molecular Oncology, Volume 19, Issue 4, Page 1029-1053, April 2025.
Advanced tumors and ineffective cancer treatments can lead to metastases in distant organs. The sole expression of microRNA 200c (miR‐200c) in breast cancer cells is shown to significantly reduce metastasis formation in xenograft mouse models. Various in vitro analyses revealed impeded migratory behavior, upon miR‐200c expression, as one prerequisite ...
Bianca Köhler, Emily Brieger, Tom Brandstätter, Elisa Hörterer, Ulrich Wilk, Jana Pöhmerer, Anna Jötten, Philipp Paulitschke, Chase P. Broedersz, Stefan Zahler, Joachim O. Rädler, Ernst Wagner, Andreas Roidl   +12 more
wiley   +1 more source

A two stage model for quantitative PCR [PDF]

arXiv, 2006
PCR (Polymerase Chain Reaction), a method which replicates a selected sequence of DNA, has revolutionized the study of genomic material, but mathematical study of the process has been limited to simple deterministic models or descriptions relying on stochastic processes.
arxiv  

Quantitation of mRNA by the polymerase chain reaction.

Proceedings of the National Academy of Sciences of the United States of America, 1989
A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which
A. Wang, M. V. Doyle, D. Mark
semanticscholar   +1 more source